Transcriptional profile of genes involved in ascorbate glutathione cycle in senescing leaves for an early senescence leaf (esl) rice mutant

Li, Zhaowei; Su, Da; Lei, Bingting; Wang, Fubiao; Wang, Geng; Pan, Gang; Cheng, Fangmin

doi:10.1016/j.jplph.2014.09.020

Cited by 33 publications

(26 citation statements)

References 53 publications

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“…D-glucose is the original material to vitamin C de novo synthesis, photosynthesis provides sufficient ingredients for the L-galactose pathway, causing higher level of expression of VTC2 and GLDH and thus higher level of vitamin C. Gallie 18 claimed that high light levels increased the production of ROS in plants. This observation might explain the higher expression level of DHAR in light germination, something that also agrees with previous research on the importance of DHAR to plant cells under different stress 17, 18, 20, 24, 26, 28, 29 .…”

Section: Resultssupporting

confidence: 92%

“…Previous research has indicated that vitamin C is generated by two ways: de novo synthesis from D-glucose, and the vitamin C recycling pathway called ascorbate-glutathione ( AsA-GSH ) cycle 24 . Three genes were selected in this study, two of which ( VTC2 , GLDH ) were from L-galactose pathway and the other one ( DHAR ) was from the AsA-GSH cycle.…”

Section: Resultsmentioning

confidence: 99%

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The manipulation of gene expression and the biosynthesis of Vitamin C, E and folate in light-and dark-germination of sweet corn seeds

Liu

Xiang

et al. 2017

Sci Rep

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This study investigates the potential interrelationship between gene expression and biosynthesis of vitamin C, E and folate in sweet corn sprouts. Germination of sweet corn kernels was conducted in light and dark environments to determine if this relationship was regulated by photo-illumination. Results indicated that light and dark environments affected the DHAR, TMT and GTPCH expression and that these genes were the predominant genes of vitamin C, E and folate biosynthesis pathways respectively during the germination. Levels of vitamin C and folate increased during the germination of sweet corn seeds while vitamin E had a declining manner. Sweet corn sprouts had higher vitamin C and E levels as well as relevant gene expression levels in light environment while illumination had little influence on the folate contents and the gene expression levels during the germination. These results indicate that there might be a collaborative relationship between vitamin C and folate regulation during sweet corn seed germination, while an inhibitive regulation might exist between vitamin C and E.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

The manipulation of gene expression and the biosynthesis of Vitamin C, E and folate in light-and dark-germination of sweet corn seeds

Liu

Xiang

et al. 2017

Sci Rep

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“…The promoter of Arabidopsis APX1 and APX2 contains a heat shock element binding a heat shock factor sensitive to endogenous H 2 O 2 production (de Pinto et al, 2015). In rice, an increase of H 2 O 2 levels enhanced the expression of two cytosolic APX genes (Li et al, 2015). For all the three analyzed c APX s the expression values returned comparable to the control after 20 h of re-oxygenation.…”

Section: Resultsmentioning

confidence: 99%

ROS Production and Scavenging under Anoxia and Re-Oxygenation in Arabidopsis Cells: A Balance between Redox Signaling and Impairment

et al. 2016

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Plants can frequently experience low oxygen concentrations due to environmental factors such as flooding or waterlogging. It has been reported that both anoxia and the transition from anoxia to re-oxygenation determine a strong imbalance in the cellular redox state involving the production of reactive oxygen species (ROS) and nitric oxide (NO). Plant cell cultures can be a suitable system to study the response to oxygen deprivation stress since a close control of physicochemical parameters is available when using bioreactors. For this purpose, Arabidopsis cell suspension cultures grown in a stirred bioreactor were subjected to a severe anoxic stress and analyzed during anoxia and re-oxygenation for alteration in ROS and NO as well as in antioxidant enzymes and metabolites. The results obtained by confocal microscopy showed the dramatic increase of ROS, H2O2, and NO during the anoxic shock. All the ascorbate-glutathione related parameters were altered during anoxia but restored during re-oxygenation. Anoxia also induced a slight but significant increase of α-tocopherol levels measured at the end of the treatment. Overall, the evaluation of cell defenses during anoxia and re-oxygenation in Arabidopsis cell cultures revealed that the immediate response involving the overproduction of reactive species activated the antioxidant machinery including ascorbate-glutathione system, α-tocopherol and the ROS-scavenging enzymes ascorbate peroxidase, catalase, and peroxidase making cells able to counteract the stress toward cell survival.

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“…Ascorbic acid (AsA) and reduced glutathione (GSH; γ- l -glutamyl- l -cysteinylglycine) are major non-enzymatic and hydrophilic antioxidants in the leaves of maize ( Zea mays L.) [ 1 , 2 ]. In the plant kingdom, the fundamental role of these compounds is linked to scavenging a broad range of reactive oxygen species (ROS) generated under physiological as well as oxidative stress conditions [ 3 , 4 ]. It has been signally reported that elevated amount of GSH in the stressed plants may influence the gene expression through thiol-mediated alternations in the structure of mitogen-activated protein kinases (MAPKs), transcription factors and RNA polymerase [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…GSH is biosynthesized in plastids and cytosol, and it is being transported via unrecognized routes to the other subcellular locations [ 7 ], whereas AsA is produced in mitochondria, and subsequently, it is translocated to the other compartments or even extracellularly through the plasma membrane [ 8 ]. The content of these metabolites varies considerably between diverse plant systems, in dependence on genotype, developmental stage and organ, tissue and subcellular localization [ 3 , 7 ]. It has been evidenced that multifarious exogenous stimuli (e.g., heavy metals, ozone, salinity, pathogens or parasites) affect foliar amounts of GSH and/or AsA, and their redox ratios in the stressed plants [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Expression Patterns of Genes Involved in Ascorbate-Glutathione Cycle in Aphid-Infested Maize (Zea mays L.) Seedlings

Sytykiewicz

2016

IJMS

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Reduced forms of ascorbate (AsA) and glutathione (GSH) are among the most important non-enzymatic foliar antioxidants in maize (Zea mays L.). The survey was aimed to evaluate impact of bird cherry-oat aphid (Rhopalosiphum padi L.) or grain aphid (Sitobion avenae F.) herbivory on expression of genes related to ascorbate-glutathione (AsA-GSH) cycle in seedlings of six maize varieties (Ambrozja, Nana, Tasty Sweet, Touran, Waza, Złota Karłowa), differing in resistance to the cereal aphids. Relative expression of sixteen maize genes encoding isoenzymes of ascorbate peroxidase (APX1, APX2, APX3, APX4, APX5, APX6, APX7), monodehydroascorbate reductase (MDHAR1, MDHAR2, MDHAR3, MDHAR4), dehydroascorbate reductase (DHAR1, DHAR2, DHAR3) and glutathione reductase (GR1, GR2) was quantified. Furthermore, effect of hemipterans’ attack on activity of APX, MDHAR, DHAR and GR enzymes, and the content of reduced and oxidized ascorbate and glutathione in maize plants were assessed. Seedling leaves of more resistant Z. mays varieties responded higher elevations in abundance of target transcripts. In addition, earlier and stronger aphid-triggered changes in activity of APX, MDHAR, DHAR and GR enzymes, and greater modulations in amount of the analyzed antioxidative metabolites were detected in foliar tissues of highly resistant Ambrozja genotype in relation to susceptible Tasty Sweet plants.

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Transcriptional profile of genes involved in ascorbate glutathione cycle in senescing leaves for an early senescence leaf (esl) rice mutant

Cited by 33 publications

References 53 publications

The manipulation of gene expression and the biosynthesis of Vitamin C, E and folate in light-and dark-germination of sweet corn seeds

The manipulation of gene expression and the biosynthesis of Vitamin C, E and folate in light-and dark-germination of sweet corn seeds

ROS Production and Scavenging under Anoxia and Re-Oxygenation in Arabidopsis Cells: A Balance between Redox Signaling and Impairment

Expression Patterns of Genes Involved in Ascorbate-Glutathione Cycle in Aphid-Infested Maize (Zea mays L.) Seedlings

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