Transcriptional profiling and localization of GUL-1, a COT-1 pathway component, in Neurospora crassa

Herold, Inbal; Kowbel, David; Delgado-Álvarez, Diego L.; Garduño-Rosales, Marisela; Mouriño-Pérez, Rosa R.; Yarden, Oded

doi:10.1016/j.fgb.2019.01.010

Cited by 13 publications

(25 citation statements)

References 69 publications

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“…Our imaging data provide compelling evidence that in fungal cells GUL1 is present on Rab5-positive transport endosomes, which shuttle along microtubules. Consistently, microtubule-dependent movement has been already described for GUL-1 from N. crassa [29,36]. Endosomal mRNA transport is well-studied in the basidiomycete Ustilago maydis and key components are the RNA-bindings proteins (RBPs) Rrm4, the poly(A)-binding protein Pab1 and the small glycine rich RRM protein Grp1 [50,60].…”

Section: Gul1 Is Trafficking On Endosomessupporting

confidence: 54%

“…GUL1, carrying an RNA-binding domain, is highly conserved within ascomycetous and basidiomycetous fungi [29,33,40]. In our phosphoproteomic analysis, we detected ten phosphorylation sites of GUL1, with phosphorylation of S180 and S510 being differentially regulated in the three mutants of this study (Table 2).…”

Section: Gul1 Is a Putative Dephosphorylation Target Of The Stripak Cmentioning

confidence: 63%

“…Dynamic live cell imaging revealed a velocity of about 2,5 μm/s (Fig 7C). GUL1-GFP movement was microtubule-dependent, since it was inhibited by benomyl ( Fig 7A and 7B, S2-S5 Movies) [29].…”

Section: Gul1 Locates Close To the Nucleus And Shuttles On Endosomesmentioning

confidence: 91%

“…A recent RNAseq analysis with GUL-1 deletion strains identified further genes involved in mycelium development, transcriptional regulation, cell wall biosynthesis, and carbohydrate metabolic processes. Finally, live imaging showed that GUL-1 movement is microtubule-dependent [ 29 ]. A homolog of GUL1 in yeast is the s uppressor of the S IT4 protein phosphatase d eletion (Ssd1), which was discovered in a mutant screen to suppress the lethality of sit4 deletion strain [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

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The STRIPAK signaling complex regulates dephosphorylation of GUL1, an RNA-binding protein that shuttles on endosomes

et al. 2020

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The striatin-interacting phosphatase and kinase (STRIPAK) multi-subunit signaling complex is highly conserved within eukaryotes. In fungi, STRIPAK controls multicellular development, morphogenesis, pathogenicity, and cell-cell recognition, while in humans, certain diseases are related to this signaling complex. To date, phosphorylation and dephosphorylation targets of STRIPAK are still widely unknown in microbial as well as animal systems. Here, we provide an extended global proteome and phosphoproteome study using the wild type as well as STRIPAK single and double deletion mutants (Δpro11, Δpro11Δpro22,

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Section: Gul1 Is Trafficking On Endosomessupporting

confidence: 54%

Section: Gul1 Is a Putative Dephosphorylation Target Of The Stripak Cmentioning

confidence: 63%

Section: Gul1 Locates Close To the Nucleus And Shuttles On Endosomesmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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The STRIPAK signaling complex regulates dephosphorylation of GUL1, an RNA-binding protein that shuttles on endosomes

et al. 2020

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“…Endosomal shuttling of the heterologous expressed Rrm4 orthologue from fungi as distant as Rhizophagus irregularis in U. maydis, suggests a high degree of functional conservation (Müller et al, 2019). Intriguingly, microtubule-dependent shuttling of the RNA-binding protein Gul1 was recently reported in hyphae of the ascomycete Neurospora crassa, although the mode of membrane association is still unclear (Herold et al, 2019).…”

Section: Membrane-associated Rna Transport As a Widespread Conceptmentioning

confidence: 99%

Inside-Out: From Endosomes to Extracellular Vesicles in Fungal RNA Transport

Kwon

Tisserant

Tulinski

et al. 2019

Preprint

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Membrane-coupled RNA transport is an emerging theme in fungal biology. This review focuses on the RNA cargo and mechanistic details of transport via two inter-related sets of organelles: endosomes and extracellular vesicles for intra- and intercellular RNA transfer. Simultaneous transport and translation of messenger RNAs (mRNAs) on the surface of shuttling endosomes is a conserved process pertinent to highly polarised eukaryotic cells, such as hyphae or neurons. Here we detail the endosomal mRNA transport machinery components and mRNA targets of the core RNA-binding protein Rrm4. Extracellular vesicles (EVs) are newly garnering interest as mediators of intercellular communication, especially between pathogenic fungi and their hosts. Landmark studies in plant-fungus interactions indicate EVs as a means of delivering various cargos, most notably small RNAs (sRNAs), for cross-kingdom RNA interference. Recent advances and implications of the nascent field of fungal EVs are discussed and potential links between endosomal and EV-mediated RNA transport are proposed.

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RNA Dialogues in Fungal–Plant Relationships

Göhre

Weiberg²

2022

The Mycota

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Transcriptional profiling and localization of GUL-1, a COT-1 pathway component, in Neurospora crassa

Cited by 13 publications

References 69 publications

The STRIPAK signaling complex regulates dephosphorylation of GUL1, an RNA-binding protein that shuttles on endosomes

The STRIPAK signaling complex regulates dephosphorylation of GUL1, an RNA-binding protein that shuttles on endosomes

Inside-Out: From Endosomes to Extracellular Vesicles in Fungal RNA Transport

RNA Dialogues in Fungal–Plant Relationships

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