Transcriptional regulation of the Saccharomyces cerevisiae amino acid permease gene BAP2

Nielsen, P. H.; Hazel, Bart van den; Didion, Thomas; Boer, Marco de; Jørgensen, Marianne U.; Planta, Rudi J.; Kielland‐Brandt, Morten C.; Andersen, H.A.

doi:10.1007/s004380000347

Cited by 55 publications

(68 citation statements)

References 22 publications

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“…LEU3 encodes a zinc-finger transcription factor that regulates genes involved in the biosynthesis and uptake of branched-chain amino acids (Friden and Schimmel 1988;Nielsen et al 2001). Strain BY4742 is a leucine auxotroph, and its growth depends on external leucine.…”

Section: Resultsmentioning

confidence: 99%

Global Screening of Genes Essential for Growth in High-Pressure and Cold Environments: Searching for Basic Adaptive Strategies Using a Yeast Deletion Library

Abe

Minegishi

2008

Genetics

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Microorganisms display an optimal temperature and hydrostatic pressure for growth. To establish the molecular basis of piezo-and psychroadaptation, we elucidated global genetic defects that give rise to susceptibility to high pressure and low temperature in Saccharomyces cerevisiae. Here we present 80 genes including 71 genes responsible for high-pressure growth and 56 responsible for low-temperature growth with a significant overlap of 47 genes. Numerous previously known cold-sensitive mutants exhibit marked high-pressure sensitivity. We identified critically important cellular functions: (i) amino acid biosynthesis, (ii) microautophagy and sorting of amino acid permease established by the exit from rapamycin-induced growth arrest/Gap1 sorting in the endosome (EGO/GSE) complex, (iii) mitochondrial functions, (iv) membrane trafficking, (v) actin organization mediated by Drs2-Cdc50, and (vi) transcription regulated by the Ccr4-Not complex. The loss of EGO/GSE complex resulted in a marked defect in amino acid uptake following high-pressure and low-temperature incubation, suggesting its role in surface delivery of amino acid permeases. Microautophagy and mitochondrial functions converge on glutamine homeostasis in the target of rapamycin (TOR) signaling pathway. The localization of actin requires numerous associated proteins to be properly delivered by membrane trafficking. In this study, we offer a novel route to gaining insights into cellular functions and the genetic network from growth properties of deletion mutants under high pressure and low temperature.

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Section: Resultsmentioning

confidence: 99%

Global Screening of Genes Essential for Growth in High-Pressure and Cold Environments: Searching for Basic Adaptive Strategies Using a Yeast Deletion Library

Abe

Minegishi

2008

Genetics

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“…Not surprisingly, several general factors that affect the expression of SPS-sensormodulated genes have been described. These include Abf1p, Leu3p, Tup1p, Ssn6p, Uga35p, ubiquitin, and the SCF-Grr1p E3 ubiquitin ligase complex (Iraqui et al 1999;de Boer et al 2000;Bernard and André 2001b;Forsberg et al 2001;Nielsen et al 2001). The hierarchy in which these factors operate to control gene expression remains to be elucidated.…”

Section: Sps-sensor-induced Stp1p/stp2p Activation Genes and Developmenmentioning

confidence: 99%

“…In detailed studies focused on the promoters of BAP2 and BAP3, an aminoacid-dependent upstream activating sequence (UAS aa ) was identified (de Boer et al 1998). Both Stp1p and Stp2p are able to bind DNA fragments containing the UAS aa (de Boer et al 2000;Nielsen et al 2001). Similar UAS aa motifs are present in the promoters of several other SPSsensor target genes.…”

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confidence: 99%

Receptor-mediated endoproteolytic activation of two transcription factors in yeast

Andréasson¹,

Ljungdahl²

2002

Genes Dev.

113

165

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Yeast possess a plasma membrane sensor of external amino acids that functions as a ligand-activated receptor. This multimeric sensor, dubbed the SPS sensor, initiates signals that regulate the expression of genes required for proper amino acid uptake. Stp1p and Stp2p are transcription factors that bind to specific sequences within the promoters of SPS-sensor-regulated genes. These factors exhibit redundant and overlapping abilities to activate transcription. We have found that Stp1p and Stp2p are synthesized as latent cytoplasmic precursors. In response to extracellular amino acids, the SPS sensor induces the rapid endoproteolytic processing of Stp1p and Stp2p. The processing of Stp1p/Stp2p occurs independently of proteasome function and without the apparent involvement of additional components. The shorter forms of these transcription factors, lacking N-terminal inhibitory domains, are targeted to the nucleus, where they transactivate SPS-sensor target genes. These results define a completely unique and streamline metabolic control pathway that directly routes environmental signals initiated at the plasma membrane to transcriptional activation in the nucleus of yeast.

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“…This liberates the DNA binding and transactivation domains from an ϳ10-kDa N-terminal regulatory fragment. The shorter forms of Stp1 and Stp2 accumulate in the nucleus and bind to specific upstream activating sequences (UAS aa ) within SPS sensor-regulated promoters (24,25).…”

mentioning

confidence: 99%

Inner Nuclear Membrane Proteins Asi1, Asi2, and Asi3 Function in Concert to Maintain the Latent Properties of Transcription Factors Stp1 and Stp2

Zargari

Boban

Heessen

et al. 2007

Journal of Biological Chemistry

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In yeast the homologous transcription factors Stp1 and Stp2 are synthesized as latent cytoplasmic precursors with N-terminal regulatory domains. In response to extracellular amino acids the regulatory domains are endoproteolytically excised by the plasma membrane-localized SPS sensor. The processed forms of Stp1 and Stp2 efficiently enter the nucleus and induce expression of amino acid permease genes. We recently reported that the inner nuclear membrane protein Asi1 is required to prevent unprocessed forms of Stp1 and Stp2, which ectopically enter the nucleus, from binding SPS sensor-regulated promoters. Here we show that Asi3, an Asi1 homolog, and Asi2 are integral proteins of the inner nuclear membrane that function in concert with Asi1. In cells lacking any of the three Asi proteins, unprocessed full-length forms of Stp1 and Stp2 constitutively induce SPS sensor-regulated genes. Our results demonstrate that the Asi proteins ensure the fidelity of SPS sensor signaling by maintaining the dormant, or repressed state, of gene expression in the absence of inducing signals. This study documents additional components of a novel mechanism controlling transcription in eukaryotic cells.In eukaryotes the nucleoplasm and cytoplasm are separated by the nuclear envelope. The nuclear envelope consists of two closely aligned bilayers, the inner and outer membranes, each with a unique set of resident proteins. The two nuclear membranes are joined at nuclear pore complexes, which function as channels that provide selective entry and exit routes across the nuclear envelope. Aside from rather detailed knowledge regarding the structure of nuclear pores, there is markedly little known regarding non-pore nuclear proteins (1). However, accumulating data indicate that nuclear envelope proteins participate in a variety of important processes including maintenance of nuclear architecture, chromatin organization, signaling, and gene expression (1-3). Significantly, mutations in genes encoding nuclear envelope proteins are linked to at least 15 inherited human diseases and syndromes (4, 5).In mammalian cells, nuclear lamins are involved in an extensive network of protein-protein interactions, including inner nuclear membrane proteins and transcriptional regulators, some of which bind DNA (3, 6). Although yeast cells lack lamin homologs (7), processes associated with the inner nuclear membrane have been shown to influence patterns of gene expression. For example, although silencing is not obligatorily linked to perinuclear anchoring (8), recruitment of chromatin to the nuclear periphery can facilitate gene repression (9). Evidence obtained using genome-wide approaches suggest that transcriptionally active regions of chromosomes localize to nuclear pore complexes (10, 11). Consistently, a number of actively expressed genes have been found to interact with the nucleoporin Nup2; the interactions occur at the promoter region of genes and appear to correlate with early events of gene expression (12, 13). Together findings in yeast and metazoan...

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Transcriptional regulation of the Saccharomyces cerevisiae amino acid permease gene BAP2

Cited by 55 publications

References 22 publications

Global Screening of Genes Essential for Growth in High-Pressure and Cold Environments: Searching for Basic Adaptive Strategies Using a Yeast Deletion Library

Global Screening of Genes Essential for Growth in High-Pressure and Cold Environments: Searching for Basic Adaptive Strategies Using a Yeast Deletion Library

Receptor-mediated endoproteolytic activation of two transcription factors in yeast

Inner Nuclear Membrane Proteins Asi1, Asi2, and Asi3 Function in Concert to Maintain the Latent Properties of Transcription Factors Stp1 and Stp2

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