Transcriptome analysis during ripening of table grape berry cv. Thompson Seedless

Abstract

Ripening is one of the key processes associated with the development of major organoleptic characteristics of the fruit. This process has been extensively characterized in climacteric fruit, in contrast with non-climacteric fruit such as grape, where the process is less understood. With the aim of studying changes in gene expression during ripening of non-climacteric fruit, an Illumina based RNA-Seq transcriptome analysis was performed on four developmental stages, between veraison and harvest, on table grapes berries cv Thompson Seedless. Functional analysis showed a transcriptional increase in genes related with degradation processes of chlorophyll, lipids, macromolecules recycling and nucleosomes organization; accompanied by a decrease in genes related with chloroplasts integrity and amino acid synthesis pathways. It was possible to identify several processes described during leaf senescence, particularly close to harvest. Before this point, the results suggest a high transcriptional activity associated with the regulation of gene expression, cytoskeletal organization and cell wall metabolism, which can be related to growth of berries and firmness loss characteristic to this stage of development. This high metabolic activity could be associated with an increase in the transcription of genes related with glycolysis and respiration, unexpected for a non-climacteric fruit ripening.

Comparison of transcriptional expression patterns of phenols and carotenoids in ‘Kyoho’ grapes under a two-crop-a-year cultivation system

“…In addition, compared with the winter grapes, the summer grapes showed a significantly greater skin-to-berry ratio at E-L 33, 35 and 37 but showed the opposite result at E-L 38 (Fig 4B). As grapes ripen, their TSS content and TA gradually increases and decreases, respectively [24]. The TSS content in the winter grapes was significantly higher than that in the summer grapes at E-L 38, although no significant difference was observed at E-L 35, 36 or 37 (Fig 4C).…”

Comparative phenotypic and transcriptomic analysis of Victoria and flame seedless grape cultivars during berry ripening

“…Further study efforts have also been directed towards understanding the grape vine berry quality, softening and cracking, as well as skin coloration [26,27]. The use of transcriptome and functional analysis during grape berry ripening have revealed numerous enzymes during fruit development and suggested an increased gene activities associated with respiration, and metabolic pathways throughout the ripening stage [28].…”

Transcriptome Changes Induced by Different Potassium Levels in Banana Roots

“…The construction method of the library was the same as that reported by Balic et al [115]. After being purified and enriched, the 200 bp ± 25 bp cDNA fragments were used to construct cDNA library.…”