Transcriptome Analysis of Canola (Brassica napus) under Salt Stress at the Germination Stage

Long, Wei; Zou, Xiling; Zhang, Xuekun

doi:10.1371/journal.pone.0116217

Cited by 48 publications

(39 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From the digital gene expression results, 826 and 224 genes were differentially expressed in CL2 and CL6 in comparison with CK, respectively. Of the DEGs, the down-regulated gene number was higher than the up-regulated gene number in CL2 and in CL6 in comparison with CK, which was opposite of the tendency in Gossypium aridum [65] and in Canola [66]; this phenomenon may be because N. sibirica Pall. is a halophyte.…”

Section: Discussionmentioning

confidence: 77%

De Novo Transcriptome Characterization, Gene Expression Profiling and Ionic Responses of Nitraria sibirica Pall. under Salt Stress

Tang

Zhu

et al. 2017

Forests

View full text Add to dashboard Cite

Nitraria sibirica Pall., a typical halophyte of great ecological value, is widely distributed in desert, saline, and coastal saline-alkali environments. Consequently, researching the salt tolerance mechanism of N. sibirica Pall. has great significance to the cultivation and utilization of salt-tolerant plants. In this research, RNA-seq, digital gene expression (DGE), and high flux element analysis technologies were used to investigate the molecular and physiological mechanisms related to salt tolerance of N. sibirica Pall. Integrative analysis and de novo transcriptome assembly generated 137,421 unigenes. In total, 58,340 and 34,033 unigenes were annotated with gene ontology (GO) terms and mapped in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, respectively. Three differentially expressed genes (DEGs) libraries were subsequently constructed from the leaves of N. sibirica Pall. seedlings under different treatments: control (CK), light short-term salt stress (CL2), and heavy long-term salt stress (CL6). Eight hundred and twenty-six, and 224 differentially expressed genes were identified in CL2 and CL6 compared to CK, respectively. Finally, ionomic analysis of N. sibirica Pall. seedlings treated with 0, 100, 200 or 300 mM concentrations of NaCl for one day showed that the uptake and distribution of Ca, Cu, Fe, Mg and K in different organs of N. sibirica Pall. were significantly affected by salt stress. Our findings have identified potential genes involved in salt tolerance and in the reference transcriptome and have revealed the salt tolerance mechanism in N. sibirica Pall. These findings will provide further insight into the molecular and physiological mechanisms related to salt stress in N. sibirica Pall. and in other halophytes.

show abstract

Section: Discussionmentioning

confidence: 77%

De Novo Transcriptome Characterization, Gene Expression Profiling and Ionic Responses of Nitraria sibirica Pall. under Salt Stress

Tang

Zhu

et al. 2017

Forests

View full text Add to dashboard Cite

show abstract

“…These studies have suggested that differentially expressed genes relating to oxidation-reduction processes and proline metabolism, as well as many transcription factors, all likely play roles in salt tolerance 25, 27 . In a recent study transcriptome-level analysis was used to examine the root responses to salinity in an Indian wheat salt-tolerant variety 29 .…”

Section: Introductionmentioning

confidence: 99%

RNAseq analysis reveals pathways and candidate genes associated with salinity tolerance in a spaceflight-induced wheat mutant

Xiong

Guo

Xie

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Salinity stress has become an increasing threat to food security worldwide and elucidation of the mechanism for salinity tolerance is of great significance. Induced mutation, especially spaceflight mutagenesis, is one important method for crop breeding. In this study, we show that a spaceflight-induced wheat mutant, named salinity tolerance 1 (st1), is a salinity-tolerant line. We report the characteristics of transcriptomic sequence variation induced by spaceflight, and show that mutations in genes associated with sodium ion transport may directly contribute to salinity tolerance in st1. Furthermore, GO and KEGG enrichment analysis of differentially expressed genes (DEGs) between salinity-treated st1 and wild type suggested that the homeostasis of oxidation-reduction process is important for salt tolerance in st1. Through KEGG pathway analysis, “Butanoate metabolism” was identified as a new pathway for salinity responses. Additionally, key genes for salinity tolerance, such as genes encoding arginine decarboxylase, polyamine oxidase, hormones-related, were not only salt-induced in st1 but also showed higher expression in salt-treated st1 compared with salt-treated WT, indicating that these genes may play important roles in salinity tolerance in st1. This study presents valuable genetic resources for studies on transcriptome variation caused by induced mutation and the identification of salt tolerance genes in crops.

show abstract

“…High salinity reduces germination, seedling emergence, and ultimately crop establishment (Stumpf et al 1986;Fowler 1991;Khan and Ungar 1999;Puppala et al 1999;Ashraf 2001;Qasim et al 2003;Ashraf and Foolad 2005;Guma et al 2010;Jamila et al 2010;Zivdar et al 2011). Ahamad et al (2012) reported salinity stress significantly affected the rate of germination, root and shoot growth of four canola cultivars, while Long et al (2015) reported that root growth of canola is affected by salinity stress as early as 12 h post-exposure. Our study confirmed that several parameters need to be considered when examining salinity tolerance, and that no single measure is sufficient when selecting a salinity-tolerant genotype.…”

Section: Salinity (Ec Sol )mentioning

confidence: 99%

“…Qasim et al (2003) observed a sharp increase in proline content in leaves of two canola cultivars, Dunkeld and Cyclone under salinity stress (4, 8, and 12 dS m −1 ) compared with low salinity stress (2 dS m −1 ). Long et al (2015) showed that proline dehydrogenase (ProDH) and Δ1-pyrroline-5-carboxylate synthase (P5CS) were differentially expressed in B. napus under salt stress. Both these enzymes are involved in proline metabolism in plants.…”

Section: Salinity (Ec Sol )mentioning

confidence: 99%

Seedling, early vegetative, and adult plant growth of oilseed rapes (Brassica napus L.) under saline stress

et al. 2019

View full text Add to dashboard Cite

Salinity is a major limiting factor for early crop establishment and yield. In this study, 131 Brassica napus genotypes were evaluated for germination and early seedling growth in Murashige & Skoog medium supplemented with NaCl. Selected genotypes were then evaluated for tolerance to salinity at the vegetative and reproductive stages in greenhouse and semi-hydroponic systems using 1.4, 5, 10, 15, 20, and 28 dS m−1 salt stress. Relative salt tolerance (RST) was calculated and compared with genotype performance under no salt stress (control). The area under the germination progress curve (AUGPC) varied from 53 to 90 in the control and from 6 to 89 under 200 mmol L−1 NaCl stress. The seedling vigor index (SVI) ranged from 200 to 1606 and 10 to 736 in the control and 200 mmol L−1 salt stress treatments, respectively. The RST for germination, root length, shoot length, and SVI ranged from 8% to 97.7%, 2% to 98.3%, 6.5% to 70.8%, and 1.9% to 83%, respectively. Root length was most severely affected by saline conditions, followed by shoot length and AUGPC, when RST percentages of these traits were compared among responses of the 131 genotypes. Genotypes showed varying levels of proline and glucosinolate accumulation under different levels of saline stress. Greater accumulation of proline and glucosinolates was recorded with increased salinity level. This study indicates that variation exists in seedling and adult plant responses to saline stress in B. napus genotypes and that improvement for salinity tolerance requires selection at the seedling, vegetative, and reproductive plant stages.

show abstract

Transcriptome Analysis of Canola (Brassica napus) under Salt Stress at the Germination Stage

Cited by 48 publications

References 72 publications

De Novo Transcriptome Characterization, Gene Expression Profiling and Ionic Responses of Nitraria sibirica Pall. under Salt Stress

De Novo Transcriptome Characterization, Gene Expression Profiling and Ionic Responses of Nitraria sibirica Pall. under Salt Stress

RNAseq analysis reveals pathways and candidate genes associated with salinity tolerance in a spaceflight-induced wheat mutant

Seedling, early vegetative, and adult plant growth of oilseed rapes (Brassica napus L.) under saline stress

Contact Info

Product

Resources

About