Transcriptome analysis of grey mullet ( Mugil cephalus ) after challenge with Lactococcus garvieae

Byadgi, Omkar; Chen, Yao-Chung; Barnes, Andrew; Tsai, Ming‐An; Wang, Pei-Chyi; Chen, Shih‐Chu

doi:10.1016/j.fsi.2016.10.006

Cited by 36 publications

(35 citation statements)

References 50 publications

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“…Despite that upregulation of il10 suppresses il8 gene expression (Ameixa & Friedland, 2001;Byadgi et al, 2016), this does not give rise to a complete blockage, also seen in the current study and by other investigators (Ameixa & Friedland, 2001;Howard et al, 1993).…”

Section: Discussionsupporting

confidence: 74%

“…Additionally, the protective effect of IL10 correlated with a marked decrease in endotoxin‐induced TNFα release (Howard et al, ). Despite that upregulation of il10 suppresses il8 gene expression (Ameixa & Friedland, ; Byadgi et al, ), this does not give rise to a complete blockage, also seen in the current study and by other investigators (Ameixa & Friedland, ; Howard et al, ).…”

Section: Discussionsupporting

confidence: 56%

“…Cross signalling between cytokines was reported in many studies (Abo-Al-Ela et al, 2017a; Byadgi et al, 2016;Falco et al, 2012;Floege, Lüscher, & Müller-Newen, 2012). IL10 suppresses T-helper 1 (Th1) cells and macrophages including their cytokines (Osugi et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…LPS is a well‐known endotoxin that can effectively evoke the immune system. LPS induces cytokine and antibody production and modulates the oxidative stress response (Abdel‐Mageid et al, ; Al‐Harbi & Austin, ; Byadgi et al, ; Kadowaki et al, ; Selvaraj, Sampath, & Sekar, ). A large number of immune‐related cDNAs were retrieved after LPS exposure in rainbow trout.…”

Section: Discussionmentioning

confidence: 99%

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Modulatory effect of lipopolysaccharide on immune‐related gene expression and serum protein fractionation in grey mullet,Mugil cephalus

et al. 2020

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Immunity occupies the top priority in aquaculture. Great efforts have been made to study fish immunity, but little is known about the immunity of grey mullet, Mugil cephalus despite of its importance. We evaluated the effect of different doses (0, 1, 10 and 100 µg/kg body weight) of lipopolysaccharide (LPS) on the expression of non‐specific immune response genes (interleukin (il) 1β, il8, il10 and tumour necrosis factor alpha (tnfα)) in the liver, as well as its effect on the fractionation of serum protein in juvenile grey mullet along different times and concentrations. Analysis revealed that LPS initially caused an upregulation in the studied cytokines. After that, transcripts levels were switched sequentially between downregulation and upregulation, ending with an upregulation at 1 week post injection in il8, il10 and tnfα. Regarding il1β, its initial upregulation was followed by gradual decreases, which reached a marked downregulation at 1 week post injection. Protein fractionation exhibited many changes among doses and time. Nine fractions were discernable in protein electrophoresis of serum in all doses. The most pronounced changes were detected in fractions 7–9 (generally refer to γ globulins or immunoglobulins) using densitometric analysis. In addition, LPS suggested to modulate the levels of serum transferrin. It is known that LPS is a component of the cell wall of Gram negative bacteria, and this study sheds light on the molecular fitness mechanisms against bacterial infection, which could help in developing a strategy to improve resistance to bacterial diseases, or possibly to enhance immune response in grey mullet.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Modulatory effect of lipopolysaccharide on immune‐related gene expression and serum protein fractionation in grey mullet,Mugil cephalus

et al. 2020

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“…This platform provides an efficient way to rapidly generate large amounts of data with low cost [ 35 , 36 ]. As a powerful method, comparative transcriptome analysis has been used to discover the molecular basis underlying specific biological events, such as immune processes during infection [ 37 , 38 ]. However, no information is available on the gene expression profiles of M .…”

Section: Introductionmentioning

confidence: 99%

A transcriptome study on Macrobrachium nipponense hepatopancreas experimentally challenged with white spot syndrome virus (WSSV)

Zhao

Sun

et al. 2018

PLoS ONE

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White spot syndrome virus (WSSV) is one of the most devastating pathogens of cultured shrimp, responsible for massive loss of its commercial products worldwide. The oriental river prawn Macrobrachium nipponense is an economically important species that is widely farmed in China and adult prawns can be infected by WSSV. However, the molecular mechanisms of the host pathogen interaction remain unknown. There is an urgent need to learn the host pathogen interaction between M. nipponense and WSSV which will be able to offer a solution in controlling the spread of WSSV. Next Generation Sequencing (NGS) was used in this study to determin the transcriptome differences by the comparison of control and WSSV-challenged moribund samples, control and WSSV-challenged survived samples of hepatopancreas in M. nipponense. A total of 64,049 predicted unigenes were obtained and classified into 63 functional groups. Approximately, 4,311 differential expression genes were identified with 3,308 genes were up-regulated when comparing the survived samples with the control. In the comparison of moribund samples with control, 1,960 differential expression genes were identified with 764 genes were up-regulated. In the contrast of two comparison libraries, 300 mutual DEGs with 95 up-regulated genes and 205 down-regulated genes. All the DEGs were performed GO and KEGG analysis, overall a total of 85 immune-related genes were obtained and these gene were groups into 13 functions and 4 KEGG pathways, such as protease inhibitors, heat shock proteins, oxidative stress, pathogen recognition immune receptors, PI3K/AKT/mTOR pathway, MAPK signaling pathway and Ubiquitin Proteasome Pathway. Ten genes that valuable in immune responses against WSSV were selected from those DEGs to furture discuss the response of host to WSSV. Results from this study contribute to a better understanding of the immune response of M. nipponense to WSSV, provide information for identifying novel genes in the absence of genome of M. nipponense. Furthermore, large number of transcripts obtained from this study could provide a strong basis for future genomic research on M. nipponense.

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Morpho-molecular identification of Heterophyes heterophyes encysted metacercariae and its immunological and histopathological effects on farmed Mugil cephalus in Egypt

et al. 2021

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Transcriptome analysis of grey mullet ( Mugil cephalus ) after challenge with Lactococcus garvieae

Abstract: 16Grey mullet (Mugil cephalus) is an economically important fish species in Taiwan

Cited by 36 publications

References 50 publications

Modulatory effect of lipopolysaccharide on immune‐related gene expression and serum protein fractionation in grey mullet,Mugil cephalus

Modulatory effect of lipopolysaccharide on immune‐related gene expression and serum protein fractionation in grey mullet,Mugil cephalus

A transcriptome study on Macrobrachium nipponense hepatopancreas experimentally challenged with white spot syndrome virus (WSSV)

Morpho-molecular identification of Heterophyes heterophyes encysted metacercariae and its immunological and histopathological effects on farmed Mugil cephalus in Egypt

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