Transcriptome and 16S rRNA analyses revealed differences in the responses of largemouth bass (Micropterus salmoides) to early Aeromonas hydrophila infection and immunization

Yuan, Xiang Yu; Zhang, Xin Tang; Xia, Yan Ting; Zhang, Ya Qi; Wang, Bei; Ye, Wen Wei; Ye, Zi Feng; Qian, Shi; Huang, Meng; Yang, Shun Fa; Fei, Hui

doi:10.1016/j.aquaculture.2021.736759

Cited by 31 publications

(16 citation statements)

References 45 publications

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“…Functional enrichment analysis, including GO and KEGG, was performed to identify the significantly enriched GO terms and metabolic pathways for DEGs (Bonferroni-corrected p -value ≤ 0.05) relative to the entire transcriptome. GO functional enrichment and KEGG pathway analyses were carried out using GOATOOLS () and KOBAS (), respectively …”

Section: Materials and Methodsmentioning

confidence: 99%

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Herbivory-Induced Emission of Volatile Terpenes in Chrysanthemum morifolium Functions as an Indirect Defense against Spodoptera litura Larvae by Attracting Natural Enemies

Jiang

Chen

et al. 2021

J. Agric. Food Chem.

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Indirect defense is an important strategy employed by sessile plants to defend against herbivorous insects by recruiting the natural enemies of herbivores mediated by herbivore-induced plant volatiles (HIPVs). We aimed to determine whether indirect defense occurs in Compositae with Chrysanthemum morifolium as the model and elucidate the mechanisms underlying the biosynthesis of HIPVs. Using two-choice olfactometer bioassays, we showed that C. morifolium plants following infestation by larvae of the tobacco cutworm (Spodoptera litura, TCW) were significantly more attractive to two natural enemies of TCW larvae than control plants, indicating that indirect defense is an active defense strategy of C. morifolium. The chemical cue responsible for indirect defense in C. morifolium was identified as a complex blend of volatiles predominated by sesquiterpenes and monoterpenes. A total of 11 candidate terpene synthase (TPS) genes were identified by comparing the transcriptomes of healthy and TCW-infested plants. Gene expression analysis confirmed that up-regulated CmjTPS genes are consistent with the elevated emission of terpenes after herbivory treatment. Our study showed that the herbivore-induced emission of JA-dependent volatile terpenes attracted both predatory and parasitic enemies of herbivores. Generally, our findings indicate that indirect defense might be an important defense mechanism against insects in C. morifolium.

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Section: Materials and Methodsmentioning

confidence: 99%

“…GO functional enrichment and KEGG pathway analyses were carried out using GOATOOLS (https://github.com/tanghaibao/Goatools) and KOBAS (http:// kobas.cbi.pku.edu.cn/home.do), respectively. 31 2.11. Quantitative Real-Time-Polymerase Chain Reaction Analysis of CmjTPS Expression.…”

Section: Methodsmentioning

confidence: 99%

Herbivory-Induced Emission of Volatile Terpenes in Chrysanthemum morifolium Functions as an Indirect Defense against Spodoptera litura Larvae by Attracting Natural Enemies

Jiang

Chen

et al. 2021

J. Agric. Food Chem.

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“…This study showed that the main genera of the gill microbiota of largemouth bass include the genus Aeromonas of the phylum Proteobacteria and an inaccurately classified genus of the Lachnospiraceae family of the phylum Firmicutes. Aeromonas is a pathogen that causes red sore in largemouth bass [44], causing an inflammatory response and gut disturbances in fish [45][46][47], which can lead to a high mortality of largemouth bass in the aquaculture system. The higher abundance of Aeromonas in this study was mainly caused by a single sample (Figure S2), and the genus Aeromonas in other gill samples was maintained at extremely low levels.…”

Section: Discussionmentioning

confidence: 99%

Interaction of Microbiota between Fish and the Environment of an In-Pond Raceway System in a Lake

et al. 2022

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Due to its ability to collect and remove aquaculture waste, an in-pond raceway system (IPRS) has been used to decrease the uncontrolled waste discharge in the traditional cage aquaculture method in large water bodies. However, when applied to large water bodies, its environmental performance is still lacking. This study focused on analyzing the microbial characteristics and the interaction between largemouth bass (gill and gut) microbiota and the environment (water and sediment) microbiota of an IPRS. Further, it revealed the primary relationship from the perspective of microbiota in the IPRS. The results show that (1) the alpha diversity of microbiota in the water is significantly lower than that of fish and sediment. The relationship between water microbiota and fish microbiota is limited. (2) The water microbiota inside and outside the tank showed high similarity and were not significantly affected by environmental factors. (3) The SourceTrack analysis showed that fish microbiota is one of the primary sources of sediment microbiota, and more than 15% of the sediment microbiota come from fish. Microbes such as Faecalibacterium, Escherichia-Shigella, and Bacteroides can significantly enrich the sediment. Our study revealed the characteristics and preliminary interaction of fish and environmental microbiota in the IPRS. It provided a reference for evaluating microbial health status in the application of IPRS in large water bodies’ aquaculture.

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“…Total RNA was extracted from spleen and head kidney of largemouth bass using RNA extraction kit (Tiangen, China) referring to manufacturer's instructions, and then fi rst-strand cDNA was synthesized using FastKing RT kit (Tiangen, China) following quality inspection of RNA by Bioanalyzer 2100 (Agilent Technologies, USA). The concentrations of cDNAs were analyzed and adjusted to 100 ng/μL, then quantitative real-time PCR (qRT-PCR) was performed in a LightCycler ® 480 II real time system (Roche, Switzerland) using SYBR Green I Master Mix (Tiangen, China) as described previously (Yuan et al, 2021). The relative abundance of MHC IIα and CD4-2 were determined with the 2 -ΔΔ C t method using β-actin gene as internal reference.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

“…Our previous study has reported that A . hydrophila infection caused metabolic disorder, high infl ammation, reduced adaptive immune response, and destruction of microbial community homeostasis of largemouth bass, resulting in high mortality (Yuan et al, 2021). The outbreaks of frequent diseases caused by A .…”

Section: Introductionmentioning

confidence: 99%

Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides

et al. 2022

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A bstractAeromonas hydrophila is a Gram-negative pathogen that can infect various fi sh, including largemouth bass ( Micropterus salmoides ), which have caused huge economic losses. In present study, largemouth bass anti-A . hydrophila antibodies were produced, then a highly immunogenic outer membrane proteins, AHA_3793, was identifi ed by combined western blotting and mass spectrometry analysis. Moreover, AHA_3793 was expressed, and its immunogenicity was further verifi ed by western blotting. Subsequently, the protective effi cacy of AHA_3793 were evaluated in largemouth bass. The results showed that rAHA_3793 could produce a relative percentage survival (RPS) of 61.76% for largemouth bass against A . hydrophila challenge. ELISA analysis showed the specifi c serum antibodies of largemouth bass against rAHA_3793 and A . hydrophila in vaccinated group in weeks 4 and 5 after immunization were signifi cantly higher than those in control group, which suggested that rAHA_3793 induced production of specifi c serum antibodies against rAHA_3793 and A . hydrophila . The qRT-PCR analysis showed that expressions of CD4-2 and MHC IIα were also signifi cantly up-regulated after immunization. These results collectively demonstrated that rAHA_3793 could induce a strong humoral immune response of largemouth bass, and then produce high immune protection eff ects against A . hydrophila infection.

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Transcriptome and 16S rRNA analyses revealed differences in the responses of largemouth bass (Micropterus salmoides) to early Aeromonas hydrophila infection and immunization

Cited by 31 publications

References 45 publications

Herbivory-Induced Emission of Volatile Terpenes in Chrysanthemum morifolium Functions as an Indirect Defense against Spodoptera litura Larvae by Attracting Natural Enemies

Herbivory-Induced Emission of Volatile Terpenes in Chrysanthemum morifolium Functions as an Indirect Defense against Spodoptera litura Larvae by Attracting Natural Enemies

Interaction of Microbiota between Fish and the Environment of an In-Pond Raceway System in a Lake

Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides

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