Transcriptome-Based Analysis Reveals a Crucial Role of BxGPCR17454 in Low Temperature Response of Pine Wood Nematode (Bursaphelenchus xylophilus)

Abstract

Background: The causal agent of pine wilt disease is the pine wood nematode (PWN) (Bursaphelenchus xylophilus), whose ability to adapt different ecological niches is a crucial determinant of their invasion to colder regions. To discover the molecular mechanism of low temperature response mechanism, we attempted to study the molecular response patterns under low temperature from B. xylophilus with a comprehensive RNA sequencing analysis and validated the differentially expressed genes (DEGs) with quantitative real-time polymerase chain reaction (qRT-PCR). Bioinformatic software was utilized to isolate and identify the low-temperature-related BxGPCR genes. Transcript abundance of six low-temperature-related BxGPCR genes and function of one of the BxGPCR genes are studied by qRT-PCR and RNA interference. Results: The results showed that we detected 432 DEGs through RNA sequencing between low-temperature-treated and ambient-temperature-treated groups nematodes. The transcript level of 6 low-temperature-related BxGPCR genes increased at low temperature. And, the survival rates of BxGPCR17454 silenced B. xylophilus revealed a significant decrease at low temperature. Conclusion: in conclusion, this transcriptome-based study revealed a crucial role of BxGPCR17454 in low temperature response process of pine wood nematode. These discoveries would assist the development of management and methods for efficient control of this devastating pine tree pest.

Transcriptome Profile Changes Associated With Heat Shock Reaction in the Entomopathogenic Nematode, Steinernema carpocapsae

“…Transcriptome technology has been used to obtain detailed information on the expression of genes in other species of nematodes. In Bursaphelenchus xylophilus and Pelodera strongyloides, the average clean reads of each treatment were 50.8 and 47.5 million, respectively (Wang et al, 2019;Zhang et al, 2019). In previous studies on the transcriptome of S. carpocapsae, nearly 15 million clean reads were obtained, and 95% of the reads were mapped to the genome using the Illumina platform.…”

Transcriptomics and coexpression network profiling of the effects of levamisole hydrochloride on Bursaphelenchus xylophilus

“…Primer 6.0 software was used to design the primers and completed by Sangon Biotech (Shanghai, China) (Additional le 9). Bx28S and Actin gene were selected as the housekeeping gene [52]. Agilent Technologies Mx3000P was used for real-time PCR detection, and qPCR was performed following the protocol provided by the TaKaRa One-Step RT-PCR Kit (Baori medical technology (Beijing) co., LTD, Beijing, China) and compared to using the threshold cycle (-ΔΔCt) method of gene expression.…”

Effect of Associated Bacteria GD1 on the Low-Temperature Adaptability of Bursaphelenchus xylophilus Based on RNA-Seq and RNAi

“…Researchers have found that this mechanism is regulated by many genes, including those associated with the cyclic guanosine phosphate (cGMP) pathway, cell autophagy pathway [ 38 ], and G protein signaling pathway [ 39 ], and have assessed the low-temperature response mechanism of PWNs. When the expression of the daf-11 and BxATG8 genes was inhibited, the survival and reproductive ability of PWNs at low temperatures decreased [ 40 , 41 ].…”