Transcriptome profiling reveals key roles of phagosome and NOD-like receptor pathway in spotting diseased Strongylocentrotus intermedius

Zhang, Weijie; Lv, Zhimeng; Li, Chenghua; Sun, Yahui; Jiang, Huijie; Zhao, Manxi; Zhao, Xuelin; Shao, Yina; Chang, Yaqing

doi:10.1016/j.fsi.2018.10.042

Cited by 33 publications

(17 citation statements)

References 37 publications

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“…Up to date, S. intermedius has become one of the most important economical invertebrate species for aquaculture in North China's sea. In China, about 1,000 mt live S. intermedius are produced yearly and the economic value amounts to about 100 million CNY (Zhang et al., 2018). This, to a large extent, relieved pressure on the wild sea urchin populations, due to alternative supplies from aquaculture (Zhang et al., 2018, 2019).…”

Section: Introductionmentioning

confidence: 99%

Effects of lipid sources on the growth performance, gonad development, fatty acid profile and transcription of related genes in juvenile sea urchin ( Strongylocentrotus intermedius )

Feng

Ding

et al. 2020

Aquacult. Nutr.

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This experiment was conducted to compare the effects of three lipid sources on the growth and gonad development in juvenile sea urchin (Strongylocentrotus intermedius). Kelp (Laminaria japonica) and three formulated feeds with the supplementation of fish oil (FO), linseed oil (LO) or soybean oil (SO) as the sole lipid source. Results showed that sea urchins fed kelp had significantly higher weight gain rate (WGR) but lower gonadosomatic index (GSI), than those fed formulated feeds (p < .05). WGR and GSI of sea urchins fed SO were significantly lower than that fed LO and FO (p < .05). The content of EPA in the gonad of sea urchins fed LO was significantly higher than that fed SO (p < .05). The content of ARA in SO was higher than that in FO and LO (p > .05). The expression of elongase 4, Δ6 desaturase and Δ5 desaturase was significantly lower in the intestine of sea urchins fed FO than that fed LO and SO (p < .05). These results indicated that LO and FO, but not SO were the ideal lipid sources for the juvenile S. intermedius. The elevated transcription of fatty acid synthesis‐related genes could be responsible for the increased EPA and ARA deposition in LO and SO, respectively.

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Section: Introductionmentioning

confidence: 99%

Effects of lipid sources on the growth performance, gonad development, fatty acid profile and transcription of related genes in juvenile sea urchin ( Strongylocentrotus intermedius )

Feng

Ding

et al. 2020

Aquacult. Nutr.

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“…Recently, large arrays of RNA sequence data have been generated and stored in public repositories such as the Short Read Archive sequence database (). Many full sea urchin mt genomes (see Table S1) and transcriptomes are there available [108,109,110,111,112,113,114,115,116]. To assess directly the alternative hypotheses outlined above, we compared the ND6 genomic sequences with the corresponding ND6 transcripts using the BLAST algorithm [35].…”

Section: Resultsmentioning

confidence: 99%

Trans-Species Polymorphism in Mitochondrial Genome of Camarodont Sea Urchins

Balakirev

2019

Genes

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Mitochondrial (mt) genomes of the sea urchins Strongylocentrotus intermedius and Mesocentrotus nudus demonstrate the identical patterns of intraspecific length variability of the ND6 gene, consisting of 489 bp (S variant) and 498 bp (L variant), respectively. For both species, the ND6 length difference is due to the 488A>G substitution, which changes the stop codon TAG in S variant for a tryptophan codon TGG in L variant and elongates the corresponding ND6 protein by three additional amino acids, Trp-Leu-Trp. The phylogenetic analysis based on mt genomes of sea urchins and related echinoderm groups from GenBank has shown the S and L ND6 variants as shared among the camarodont sea urchins; the rest of the echinoderms demonstrate the S variant only. The data suggest that the ND6 488A>G substitution can be the first example of the trans-species polymorphism in sea urchins, persisting at least since the time of the Odontophora diversification at the Eocene/Oligocene boundary (approximately 34 million years ago), which was characterized by an abrupt climate change and significant global ocean cooling. Alternative hypotheses, including the convergent RNA editing and/or codon reassignment, are not supported by direct comparisons of the ND6 gene sequences with the corresponding transcripts using the basic local alignment search tool (BLAST) of full sea urchin transcriptomes.

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“…The increased incidence of bacteria causes black-mouth and spotting diseases greatly decreased the production of S . intermedius aquaculture 1 , 4 , 8 . We found that S .…”

Section: Discussionmentioning

confidence: 99%

“…4 B). The enlarging spotting lesions commonly cause ulceration on the body wall and finally result in death 8 . Sea urchin without disease performance is shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

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Effects of the brown algae Sargassum horneri and Saccharina japonica on survival, growth and resistance of small sea urchins Strongylocentrotus intermedius

Yang

Ding

et al. 2020

Sci Rep

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Mass mortality of the long line culture of the sea urchin Strongylocentrotus intermedius in summer, which is greatly associated with their disease, energy storage and resistant abilities, is the most serious problem for the development of the aquaculture. Here, a feeding experiment was conducted for ~ 9 weeks to investigate the survival, growth and gonadal development of small S . intermedius (~ 3 cm) fed either brown algae Sargassum horneri or Saccharina japonica . Subsequently, we assessed their resistant abilities via observing the behaviors of righting, tube feet extension and Aristotle's lantern reflex at both moderately elevated and acutely changed water temperatures. Sea urchins fed S . horneri showed significantly fewer diseased individuals and slower gonadal development than those fed S . japonica . Consistently, significantly greater Aristotle's lantern reflex occurred in sea urchins fed S . horneri at moderately elevated temperatures. These findings suggest that S . horneri has direct application potential as food for the long line culture of S . intermedius in summer because of the advantage in health, energy storage (avoid the energy loss caused by gonadal development at small body sizes) and resistance abilities. In comparison, sea urchins fed S . japonica outperformed those fed S . horneri for all experimental behaviors under the acutely changed water temperatures. These findings clearly suggest that S . intermedius fed S . japonica is more suitable for the areas with cold water mass in summer, because it can effectively avoid or reduce the negative impacts of acute changes of water temperature on sea urchins. The present study provides valuable information into the management of the long line culture of S . intermedius in summer.

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Transcriptome profiling reveals key roles of phagosome and NOD-like receptor pathway in spotting diseased Strongylocentrotus intermedius

Cited by 33 publications

References 37 publications

Effects of lipid sources on the growth performance, gonad development, fatty acid profile and transcription of related genes in juvenile sea urchin ( Strongylocentrotus intermedius )

Effects of lipid sources on the growth performance, gonad development, fatty acid profile and transcription of related genes in juvenile sea urchin ( Strongylocentrotus intermedius )

Trans-Species Polymorphism in Mitochondrial Genome of Camarodont Sea Urchins

Effects of the brown algae Sargassum horneri and Saccharina japonica on survival, growth and resistance of small sea urchins Strongylocentrotus intermedius

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