Transcriptome sequencing identifies genes associated with invasion of ovarian cancer

Peng, Xiandong; Yu, Min; Chen, Jiazhou

doi:10.1177/0300060520950912

Cited by 10 publications

(7 citation statements)

References 34 publications

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“…The mutation frequency of LAMC2 is 12% in OC and LAMC2 expression is upregulated in OC tissues. LAMC2 expression was upregulated in SKOV-3 (ovarian serous carcinoma cell line) compared with MCV152 (benign ovarian epithelial tumor line)[ 36 ]. LAMC2 expression upregulation was also confirmed in OC tissues and cells.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of the Expression and Prognosis for Laminin Genes in Ovarian Cancer

Diao

Yang

2021

Pathol. Oncol. Res.

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Survival is low in ovarian cancer (OC). Most OC patients demonstrate advanced metastases, and recurrence is common. Dysregulation of laminin interactions is associated with cancer development. However, it is unknown whether laminin subunits can be considered as biomarkers for OC diagnosis, prognosis, and treatment. We used cBioPortal, GEO, ONCOMINE, GEPIA, Human Protein Atlas, Kaplan-Meier Plotter, TIMER, and Metascape to determine the associations among laminin expression, prognosis, and immune cell infiltration in OC. LAMA5, LAMB3, and LAMC2 mRNAs and LAMA3, LAMB1/B2/B3, and LAMC1/C2 proteins were overexpressed in OC tissues compared with normal ovaries. LAMA4, LAMB1, and LAMC1 mRNA upregulation was positively correlated with worse overall survival (OS) and progression-free survival (PFS) in OC. Elevated LAMA2 and LAMC2 mRNA expression levels were related to better PFS or OS, respectively. The results speculated that LAMA5 could potentially be a good prognostic factor in OC. Its expression proves valuable for predicting OS in patients diagnosed with stage Ⅳ and grade 3 OC and PFS in patients diagnosed with all OC stages or grades. LAMB3 and LAMC2 expression was correlated with platinum resistance development. ROC analysis of laminins in OC sets revealed that LAMA2/A4/A5, LAMB1/B2/B3, and LAMC2 could be used to differentiate between malignant tumors and non-neoplastic tissues. LAMA1/A5 and LAMC1 were significantly and negatively correlated with various tumor immune infiltrates (TILs), especially with dendritic cells, CD8+ T cells or neutrophil. LAMA4 and LAMB1 might be associated with tumor purity in OC. Overall, LAMA5 and LAMC1 could help predict OC survival and diagnosis and might be deemed important OC oncogenes.

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Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of the Expression and Prognosis for Laminin Genes in Ovarian Cancer

Diao

Yang

2021

Pathol. Oncol. Res.

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“…And COL4A2 expression had the most significantly positive correlations with the existence of macrophages and dendritic cell in cervical cell carcinoma [8]. COL4A3 is up-regulated in ovarian cancer [40]. And COL4A3 was an angiogenesis related gene of kidney renal clear cell carcinoma [41].…”

Section: Discussionmentioning

confidence: 97%

Integrate analysis of the prognostic values of COL4As to human gastric cancer

Zheng¹,

Chen²,

Liu³

et al. 2021

Preprint

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Collagen type IV (Col IV) is the main constituent of the basement membrane. Under physiological conditions, Col IV plays an important role in maintaining epithelial integrity and stabilizing epithelial function of the gastric mucosa. Under pathological conditions, Col IV can be free from the basement membrane under the influence of tumor cells and play a pro-metastatic role. Although there is a increasing number of investigations on Col IV, no studies to date have directly uncovered the prognostic role and potential regulatory role of the six isoforms of Col IV in gastric cancer. In the present experiment, we aimed to analyze the role of COL4A family genes in gastric cancer. COL4A1/2/3/4 was significantly overexpressed, while COL4A5/6 was decreased in gastric cancer tissues according to TCGA data and our immunohistochemical staining results. And COL4A1 had a positive correlation with tumor stage, while COL4A5/6 had negative correlations with tumor stage. COL4A1/2/4/5/6 can be considered as a diagnostic indicator of gastric cancer. High levels of COL4A1/2/4/5/6 expression may be predictive of a poor prognosis of gastric cancer. The percentages of genetic alterations in COL4As for stomach cancer varied from 3 to 17% based on the TCGA data (COL4A1, 10%; COL4A2, 8%; COL4A3, 3%; COL4A4, 5%; COL4A5, 17%; COL4A6, 17%). Besides, COL4As may modulate tumor progression by participating in classical cancer pathways: COL4A1/2/3/4/5/6 can activate the EMT process; COL4A2/3/4/6 can inhibit apoptosis and cell cycle; COL4A3/4/5 can activate the PI3K/AKT signaling pathway. This study implied that COL4As have diagnostic and prognostic value for gastric cancer.

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“…This process, driven by TGF/COL6A1 signaling, promotes cell metastasis, invasion, and migration [ 38 ]. In ovarian cancer cells, COL6A1 has been identified as an effector gene that promotes invasion and metastasis [ 39 , 40 ]. The upregulation of COL6A1 and TGFB1 expression in a hypoxic microenvironment may enhance cell proliferation [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of exosomes secreted during the epithelial-mesenchymal transition and potential biomarkers of mesenchymal high-grade serous ovarian carcinoma

Ferreira,

Thomé,

Izumi

et al. 2023

J Ovarian Res

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Background The epithelial-mesenchymal transition (EMT) promotes cell signaling and morphology alterations, contributing to cancer progression. Exosomes, extracellular vesicles containing proteins involved in cell-cell communication, have emerged as a potential source of biomarkers for several diseases. Methods Our aim was to assess the proteome content of exosomes secreted after EMT-induction to identify potential biomarkers for ovarian cancer classification. EMT was induced in the ovarian cancer cell line CAOV3 by treating it with EGF (10 ng/mL) for 96 h following 24 h of serum deprivation. Subsequently, exosomes were isolated from the supernatant using selective centrifugation after decellularization, and their characteristics were determined. The proteins present in the exosomes were extracted, identified, and quantified using Label-Free-Quantification (LFQ) via Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). To identify potential biomarkers, the obtained proteomic data was integrated with the TGGA database for mRNA expression using principal component analysis and a conditional inference tree. Results The exosomes derived from CAOV3 cells exhibited similar diameter and morphology, measuring approximately 150 nm, regardless of whether they were subjected to EMT stimulation or not. The proteomic analysis of proteins from CAOV3-derived exosomes revealed significant differential regulation of 157 proteins, with 100 showing upregulation and 57 downregulation upon EMT induction. Further comparison of the upregulated proteins with the TCGA transcriptomic data identified PLAU, LAMB1, COL6A1, and TGFB1 as potential biomarkers of the mesenchymal HGSOC subtype. Conclusions The induction of EMT, the isolation of exosomes, and the subsequent proteomic analysis highlight potential biomarkers for an aggressive ovarian cancer subtype. Further investigation into the role of these proteins is warranted to enhance our understanding of ovarian cancer outcomes.

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Transcriptome sequencing identifies genes associated with invasion of ovarian cancer

Cited by 10 publications

References 34 publications

Comprehensive Analysis of the Expression and Prognosis for Laminin Genes in Ovarian Cancer

Comprehensive Analysis of the Expression and Prognosis for Laminin Genes in Ovarian Cancer

Integrate analysis of the prognostic values of COL4As to human gastric cancer

Proteomic analysis of exosomes secreted during the epithelial-mesenchymal transition and potential biomarkers of mesenchymal high-grade serous ovarian carcinoma

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