Transcriptomic analysis of Momordica charantia polysaccharide on streptozotocin-induced diabetic rats

Bai, Wenjing; Zhang, Cong; Chen, Hong‐Man

doi:10.1016/j.gene.2018.06.106

Cited by 14 publications

(8 citation statements)

References 44 publications

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“…Msmo1 is a key cholesterol biosynthetic enzyme. Nsdhl regulates adipogenesis via a synergized expression pattern with Msmo1 [64][65][66][67]. Herein, hypo-salinity up-regulated both nsdhl and msmo1, indicating that hypo-salinity can affect adipogenesis in greater amberjack.…”

Section: Degs Involved In Steroid Biosynthesis and Lipid Metabolismmentioning

confidence: 77%

RNA Sequencing Analysis Reveals Divergent Adaptive Response to Hypo- and Hyper-Salinity in Greater Amberjack (Seriola dumerili) Juveniles

Peng

Shi

Liu

et al. 2022

Animals

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Salinity significantly affects physiological and metabolic activities, breeding, development, survival, and growth of marine fish. The greater amberjack (Seriola dumerili) is a fast-growing species that has immensely contributed to global aquaculture diversification. However, the tolerance, adaptation, and molecular responses of greater amberjack to salinity are unclear. This study reared greater amberjack juveniles under different salinity stresses (40, 30, 20, and 10 ppt) for 30 days to assess their tolerance, adaptation, and molecular responses to salinity. RNA sequencing analysis of gill tissue was used to identify genes and biological processes involved in greater amberjack response to salinity stress at 40, 30, and 20 ppt. Eighteen differentially expressed genes (DEGs) (nine upregulated and nine downregulated) were identified in the 40 vs. 30 ppt group. Moreover, 417 DEGs (205 up-regulated and 212 down-regulated) were identified in the 20 vs. 30 ppt group. qPCR and transcriptomic analysis indicated that salinity stress affected the expression of genes involved in steroid biosynthesis (ebp, sqle, lss, dhcr7, dhcr24, and cyp51a1), lipid metabolism (msmo1, nsdhl, ogdh, and edar), ion transporters (slc25a48, slc37a4, slc44a4, and apq4), and immune response (wnt4 and tlr5). Furthermore, KEGG pathway enrichment analysis showed that the DEGs were enriched in steroid biosynthesis, lipids metabolism, cytokine–cytokine receptor interaction, tryptophan metabolism, and insulin signaling pathway. Therefore, this study provides insights into the molecular mechanisms of marine fish adaptation to salinity.

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Section: Degs Involved In Steroid Biosynthesis and Lipid Metabolismmentioning

confidence: 77%

RNA Sequencing Analysis Reveals Divergent Adaptive Response to Hypo- and Hyper-Salinity in Greater Amberjack (Seriola dumerili) Juveniles

Peng

Shi

Liu

et al. 2022

Animals

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“…The antidiabetic activity of BME is higher than that of MPE because its fruit containing p-insulin polypeptide which works like insulin and charantin which can increase glucose in liver cells and muscles, 12,25 has a component that works similar to sulfonylurea. 26 Histology results of normal cell pancreas are shown with a rounded cell shape with a clear nucleus, uniform cell shape, and do not experience edema.…”

Section: Discussionmentioning

confidence: 98%

“…11 Fruit extracts can increase insulin secretion and protect the pancreas, inhibit α-glucosidase and inhibit α-amylase. [12][13][14] Innovations in oral antidiabetic agents from natural ingredients have been developed. The use of a single dose in the treatment of diabetes is often considered to lack a strong effect.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic and Antioxidant Effect Combination Vasconcellea pubescens A.DC. and Momordica charantia L. Extract in Alloxan- Induced Diabetic Rats

Sasongko¹,

Lestari²,

Yugatama³

et al. 2020

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Introduction: Mountain papaya (Vasconcellea pubescens A.DC.) and bitter melon (Momordica charantia L.) fruit extract have total flavonoids and some metabolite from its contains. The use of a single dose in the treatment of diabetes is often considered to lack a strong effect. Giving a combination of the compound is a solution to provide a synergistic effect in treatment. Material and Methods: Rat were divided into eight groups (normal, negative control, glibenclamide as a positive control, and five dose extract group). The single dose mountain papaya extract (MPE) and bitter melon extract (BME) were given at 174 mg/kg b.w of MPE, and 380 mg/kg b.w of BME in oral administration.

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“…Insulin, glucose, free fatty acids, and other nutrients have been found to affect the expression of the INSIG1 gene in recent research [ 18 ]. MSMO1 operates function in the regulation of adipogenesis and energy metabolism, MSMO1 was identified as a good candidate to adjust blood glucose levels after an integrated analysis of differential gene expression and biological functions [ 19 ]. At the same time, MSMO, FDFT1, SQLE, HMGCR and HSD17B7 are five enzymes in the cholesterol synthesis pathway [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Exploring differentially expressed genes related to metabolism by RNA-Seq in porcine embryonic fibroblast after insulin treatment

Yao

Wang

et al. 2022

J Vet Sci

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Background Insulin regulates glucose homeostasis and has important effects on metabolism, cell growth, and differentiation. Depending on the cell type and physiological context, insulin signal has specific pathways and biological outcomes in different tissues and cells. For studying the signal pathway of insulin on glycolipid metabolism in porcine embryonic fibroblast (PEF), we used high-throughput sequencing to monitor gene expression patterns regulated by insulin. Objectives The goal of our research was to see how insulin affected glucose and lipid metabolism in PEFs. Methods We cultured the PEFs with the addition of insulin and sampled them at 0, 48, and 72 h for RNA-Seq analysis in triplicate for each time point. Results At 48 and 72 h, 801 and 1,176 genes were differentially expressed, respectively. Of these, 272 up-regulated genes and 264 down-regulated genes were common to both time points. Gene Ontology analysis was used to annotate the functions of the differentially expressed genes (DEGs), the biological processes related to lipid metabolism and cell cycle were dominant. And the DEGs were significantly enriched in interleukin-17 signaling pathway, phosphatidylinositol-3-kinase-protein kinase B signaling pathway, pyruvate metabolism, and others pathways related to lipid metabolism by Kyoto Encyclopedia of Genes and Genomes enrichment analysis. Conclusions These results elucidate the transcriptomic response to insulin in PEF. The genes and pathways involved in the transcriptome mechanisms provide useful information for further research into the complicated molecular processes of insulin in PEF.

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Transcriptomic analysis of Momordica charantia polysaccharide on streptozotocin-induced diabetic rats

Cited by 14 publications

References 44 publications

RNA Sequencing Analysis Reveals Divergent Adaptive Response to Hypo- and Hyper-Salinity in Greater Amberjack (Seriola dumerili) Juveniles

RNA Sequencing Analysis Reveals Divergent Adaptive Response to Hypo- and Hyper-Salinity in Greater Amberjack (Seriola dumerili) Juveniles

Antidiabetic and Antioxidant Effect Combination Vasconcellea pubescens A.DC. and Momordica charantia L. Extract in Alloxan- Induced Diabetic Rats

Exploring differentially expressed genes related to metabolism by RNA-Seq in porcine embryonic fibroblast after insulin treatment

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