Transcriptomic changes in the pre-implantation uterus highlight histotrophic nutrition of the developing marsupial embryo

Whittington, Camilla M.; O’Meally, Denis; Laird, Melanie K.; Belov, Katherine; Thompson, Michael B.; McAllan, Bronwyn M.

doi:10.1038/s41598-018-20744-z

Cited by 29 publications

(20 citation statements)

References 95 publications

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“…These observations strongly support the view that circRNAs' expression occurs in a specie-specific, tissue-specific, and developmental stagespecific manner. Importantly, we identified 17 circRNAs that were differentially expressed in the gilts, for which we speculated that some parental genes of differentially regulated circRNAs could influence the fertility and production traits of female mammals, such as ESR1 (Handa and Weiser, 2014), DENND1A (McAllister et al, 2014), RALGPS1 (Cochran et al, 2013), and MAML2 (Whittington et al, 2018). In addition, after identifying the miRNA targets of each differentially regulated circRNA, we found that some candidate miRNAs targeted by several circRNAs are linked to mammalian development of sex differentiation and maturation.…”

Section: Discussionmentioning

confidence: 99%

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

et al. 2020

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Being the center of the hypothalamus-pituitary-ovary (HPO) axis, the pituitary plays a key role in the onset of puberty. Recent studies show that circular RNAs (circRNAs) can perform as miRNA sponges to regulate development in animals. However, the function of pituitary-derived circRNAs in first estrus remains unclear in pigs. In this study, we performed a genome-wide identification and characterization of circRNAs using pituitaries from Landrace × Yorkshire crossbred pigs at three stages: pre-, in-, and post-puberty, to describe such pituitary-derived circRNAs in pigs. A total of 5148 circRNAs were found in the gilts' pituitaries, averaging 18 682 bp in genomic distance, which consisted of approximately 91% exonic, 6% intergenic, and 3% intronic circRNAs. Furthermore, 158 novel circRNAs were identified for the first time and classified as putative pituitary-specific circRNAs. Their expression levels during the onset of puberty, significantly exceeded those of the other circRNAs, and the parental genes of these putative pituitary-specific circRNAs were enriched in "ssc04917: prolactin signaling pathway," "ssc04080: neuroactive ligand-receptor interaction," and "ssc04728: dopaminergic synapse" pathways, all of which were consistent with pituitary functioning. Additionally, 17 differentially regulated circRNAs were found and investigated for their potential interaction with miRNAs, along with genes, by constructing a circRNA-targeted miRNA-gene network. Taken together, these results provide new insight into the circRNA-mediated timing of puberty in gilts at the pituitary level.

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Section: Discussionmentioning

confidence: 99%

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

et al. 2020

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“…We searched the dunnart uterine transcriptome (Whittington et al, ) for claudin‐like sequences using BLAST searches of Didelphimorphia and Dasyuromorphia claudin sequences ( Monodelphis domestica and Sarcophilus harrisii ) from NCBI's GenBank. Claudin‐1, ‐3, ‐4, and ‐5 were all expressed in dunnart uterus.…”

Section: Resultsmentioning

confidence: 99%

“…(f) Negative control (uterine tissue of S. crassicaudata; primary antibody replaced by nonimmune anti-rabbit IgG). No staining was seen in any uterine region at any stage of the reproductive cycle in the control tissues [Color figure can be viewed at wileyonlinelibrary.com] Chemidoc MP imaging system (Biorad) using enhanced chemiluminescence (ECL plus Western blot analysis Detection System; GE Healthcare).3 | RESULTS3.1 | Comparison with transcriptomeWe searched the dunnart uterine transcriptome(Whittington et al, 2018) for claudin-like sequences using BLAST searches of Didelphimorphia and Dasyuromorphia claudin sequences (Monodelphis domestica and Sarcophilus harrisii) from NCBIʼs GenBank. Claudin-1, -3, -4, and -5 were all expressed in dunnart uterus.…”

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confidence: 99%

Dynamic changes to claudins in the uterine epithelial cells of the marsupial Sminthopsis crassicaudata (Dasyuridae) during pregnancy

Buddle

Thompson

Lindsay

et al. 2019

Molecular Reproduction Devel

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The fluid that surrounds the embryo in the uterus contains important nourishing factors and secretions. To maintain the distinct microenvironment in the uterine lumen, the tight junctions between uterine epithelial cells are remodeled to decrease paracellular movement of molecules and solutes. Modifications to tight junctions between uterine epithelial cells is a common feature of pregnancy in eutherian mammals, regardless of placental type. Here we used immunofluorescence microscopy and western blot analysis to describe distributional changes to tight junctional proteins, claudin‐1, ‐3, ‐4, and ‐5, in the uterine epithelial cells of a marsupial species, Sminthopsis crassicaudata. Immunofluorescence microscopy revealed claudin‐1, ‐3, and ‐5 in the tight junctions of the uterine epithelium of S. crassicaudata during pregnancy. These specific claudins are associated with restricting passive movement of fluid between epithelial cells in eutherians. Hence, their function during pregnancy in S. crassicaudata may be to maintain the uterine luminal content surrounding developing embryos. Claudin‐4 disappears from all uterine regions of S. crassicaudata at the time of implantation, in contrast with the distribution of this claudin in some eutherian mammals. We conclude that like eutherian mammals, distributional changes to claudins in the uterine epithelial cells of S. crassicaudata are necessary to support pregnancy. However, the combination of individual claudin isoforms in the tight junctions of the uterine epithelium of S. crassicaudata differs from that of eutherian mammals. Our findings suggest that the precise permeability of the paracellular pathway of the uterine epithelium is species‐specific.

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“…Sharma S et al reported that hsa-mir-191-5p targets the SOX4 gene and is a key signaling pathway in oncogenesis [17]. Moreover, Camilla M. Whittington et al reported the downregulation of SOX4, a transcription factor, during early pregnancy in the uteri of both Monodelphis domestica and Sminthopsis crassicaudata [18]. Our results show that the level of hsa-mir-191-5p in sperm is closely related to the process of fertilization and early development of the embryo.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Expression Profiles Analysis in Sperm Reveals hsa-mir-191 is an Auspicious Omen of in Vitro Fertilization

Xu¹,

Wang²,

Wang³

et al. 2019

Preprint

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Background : MicroRNAs (miRNAs) are a class of noncoding small RNAs that play important roles in many physiological processes by regulating gene expression. Previous studies have shown that the expression levels of total miRNAs increase during mouse embryonic development, and some miRNAs control the regulatory network in development progression. However, few studies have focused on the effects of miRNAs on early human embryonic development. The relationship between miRNAs and early human embryogenesis is still unknown. Results: In this study, sperm samples from 102 patients with a normal sperm index but treated with assisted reproductive technology (ART) were collected for small RNA sequencing, and the relationships between differentially expressed small RNAs and the fertilization rate (FR), blastocyst rate and high-quality embryo rate (HQER) were analyzed. The sperm samples with high hsa-mir-191 expression had a higher FR, effective embryo rate (EER) and HQER. hsa-mir-191 was used as a single indicator to predict the HQER. The receiver operating characteristic (ROC) curve had an area under the ROC curve (AUC) of 0.686. We also found that hsa-mir-191 expression is correlated with an abnormal sperm rate (cor = 0.29, p < 0.01). We also evaluated the relationship between hsa-mir-34c and early human embryo development in these 102 sperm samples and obtained negative results. Conclusions: These findings suggest that high hsa-mir-191-5p expression is associated with improved early human embryonic development and that hsa-mir-191-5p could be used as a potential marker to screen high-quality sperm to improve the success rates of in vitro fertilization (IVF).

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Transcriptomic changes in the pre-implantation uterus highlight histotrophic nutrition of the developing marsupial embryo

Cited by 29 publications

References 95 publications

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

Dynamic changes to claudins in the uterine epithelial cells of the marsupial Sminthopsis crassicaudata (Dasyuridae) during pregnancy

MicroRNA Expression Profiles Analysis in Sperm Reveals hsa-mir-191 is an Auspicious Omen of in Vitro Fertilization

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