Transcriptomic profiles in peripheral blood between women with unexplained recurrent implantation failure and recurrent miscarriage and the correlation with endometrium: A pilot study

Abstract

AimTo study the transcriptome profiles in the blood of recurrent implantation failure (RIF), recurrent miscarriage (RM) and fertile women during the window of implantation, and further analysis the correlation of transcriptome profiles between blood and endometrium.MethodsThis is an observational prospective study. In total 9 subjects were recruited, 3 RIF, 3 RM, and 3 controls. Paired samples (endometrium and peripheral blood) from the same subjects were precisely timed on the 7th days after luteal hormone surge (LH+7). RNA sequencing was applied to investigate the transcriptome profiles.ResultsThe results of transcriptome in peripheral blood cannot be used to characterize women with RIF and unexplained RM. There was a medium level correlation between transcriptome in peripheral blood and endometrium during the window of implantation.ConclusionThe differential transcriptome patterns in blood are not representative of those in endometrium, and the blood transcriptome cannot differentiate among the women with RIF, RM or fertile.

Uterine Transcriptome: Understanding Physiology and Disease Processes

“…Transcription profiles have been compared within the endometrium from control subjects to those with recurrent implantation failure, but also to the peripheral blood transcriptome. Huang et al [ 52 ] reported that in controls and those with recurrent implantation failure, transcriptome profiles of peripheral blood correlated with endometrium for about 10% of genes. While endometrium profiles were distinct between controls and those with recurrent miscarriages or recurrent implantation failure, peripheral blood samples were harder to distinguish between these groups.…”

Proteomic analysis methods and their role in the diagnosis of obstetric and gynecological pathology

“…Были изучены транскриптомные профили в крови и образцах эндометрия у женщин с привычным невынашиванием беременности в анамнезе. Однако стремление к неинвазивному методу и исследование только крови не представляется возможным, так как для изучения динамических и быстро меняющихся молекулярных событий в эндометрии понадобится ежедневное транскриптомное исследование крови, которое необходимо для сопоставления изменений, приводящих к недостаточности репродуктивной системы исследуемых женщин [51].…”

The Investigation of Metabonomic Pathways of Serum of Iranian Women with Recurrent Miscarriage Using 1H NMR

“…Earlier workers have shown the use of serum metabonomics with 1 H NMR in women with idiopathic recurrent spontaneous miscarriage during the implantation window [ 27 ] and identified some amino acids associated with it. Efforts have been made on transcriptomic studies to differentiate women with recurrent miscarriages and repeated implantation failures and fertile women to no avail [ 28 ].…”