Transdermal transport pathway creation: Electroporation pulse order

Becker, Sid; Zorec, Barbara; Miklavčič, Damijan; Pavšelj, Nataša

doi:10.1016/j.mbs.2014.07.001

Cited by 34 publications

(22 citation statements)

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“…Flexible control of the cell membrane permeability to impermeable molecules by means of electroporation offers a vast array of applications, including, but not limited to, biotechnology, food processing and medicine (Mahnič-Kalamiza, Vorobiev & Miklavčič, 2014; Yarmush et al, 2014; Kotnik et al, 2015; Golberg et al, 2016b). Current state of the art of the biomedical electroporation involves application of various electrode array configurations (Campana et al, 2016b; Ongaro et al, 2016; Szczurkowska et al, 2016) for both invasive (i.e., tissue ablation Reberšek et al, 2014; Golberg et al, 2016a; Klein et al, 2016) and non-invasive (i.e., transdermal) electroporation applications (Blagus et al, 2013; Zorec et al, 2013; Becker et al, 2014; Chen et al, 2015). However, these applications require direct contact between the applicator, electrodes and the biological sample.…”

Section: Introductionmentioning

confidence: 99%

Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses

Novickij

Dermol

Grainys

et al. 2017

PeerJ

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BackgroundCell membrane permeabilization by pulsed electromagnetic fields (PEMF) is a novel contactless method which results in effects similar to conventional electroporation. The non-invasiveness of the methodology, independence from the biological object homogeneity and electrical conductance introduce high flexibility and potential applicability of the PEMF in biomedicine, food processing, and biotechnology. The inferior effectiveness of the PEMF permeabilization compared to standard electroporation and the lack of clear description of the induced transmembrane transport are currently of major concern.MethodsThe PEMF permeabilization experiments have been performed using a 5.5 T, 1.2 J pulse generator with a multilayer inductor as an applicator. We investigated the feasibility to increase membrane permeability of Chinese Hamster Ovary (CHO) cells using short microsecond (15 µs) pulse bursts (100 or 200 pulses) at low frequency (1 Hz) and high dB/dt (>106 T/s). The effectiveness of the treatment was evaluated by fluorescence microscopy and flow cytometry using two different fluorescent dyes: propidium iodide (PI) and YO-PRO®-1 (YP). The results were compared to conventional electroporation (single pulse, 1.2 kV/cm, 100 µs), i.e., positive control.ResultsThe proposed PEMF protocols (both for 100 and 200 pulses) resulted in increased number of permeable cells (70 ± 11% for PI and 67 ± 9% for YP). Both cell permeabilization assays also showed a significant (8 ± 2% for PI and 35 ± 14% for YP) increase in fluorescence intensity indicating membrane permeabilization. The survival was not affected.DiscussionThe obtained results demonstrate the potential of PEMF as a contactless treatment for achieving reversible permeabilization of biological cells. Similar to electroporation, the PEMF permeabilization efficacy is influenced by pulse parameters in a dose-dependent manner.

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Section: Introductionmentioning

confidence: 99%

Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses

Novickij

Dermol

Grainys

et al. 2017

PeerJ

Self Cite

View full text Add to dashboard Cite

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“…with σ i (cm −1 Ω −1 ) the electrical conductivities in the layer i [8]. At the interfaces we assume an electrically perfect contact and we have continuity of potential and fluxes:…”

Section: The Electric Potential Fieldmentioning

confidence: 99%

Iontophoretic transdermal drug delivery: a multi-layered approach

et al. 2016

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We present a multi-layer mathematical model to describe the the transdermal drug release from an iontophoretic system. The Nernst-Planck equation describe the basic convectiondiffusion process, with the electric potential obtained by Laplace equation. These equations are complemented with suitable interface and boundary conditions in a multi-domain. The stability of the mathematical problem is discussed in different scenarios and a finite-difference method is used to solve the coupled system. Numerical experiments are included to illustrate the drug dynamics under different conditions.

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“…Some of these methods rely on the physical disturbance of the SC layer (e.g. using microneedles (MNs) [10] or electroporation [11]) whereas some others deal with chemical modifications of therapeutic agents or the SC layer for enhanced skin penetration [7,12,13]. Hair follicles have also been explored as another transport channel for transdermal delivery of gene and drugs [14,15].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Skin Penetration Enhancers for Transdermal Gene and Drug Delivery

Amjadi

Mostaghaci

Sitti

2017

CGT

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There is a growing interest in transdermal delivery systems because of their noninvasive, targeted, and on-demand delivery of gene and drugs. However, efficient penetration of therapeutic compounds into the skin is still challenging largely due to the impermeability of the outermost layer of the skin, known as stratum corneum. Recently, there have been major research activities to enhance the skin penetration depth of pharmacological agents. This article reviews recent advances in the development of various strategies for skin penetration enhancement. We show that approaches such as ultrasound waves, laser, and microneedle patches have successfully been employed to physically disrupt the stratum corneum structure for enhanced transdermal delivery. Rather than physical approaches, several non-physical route have also been utilized for efficient transdermal delivery across the skin barrier. Finally, we discuss some clinical applications of transdermal delivery systems for gene and drug delivery. This paper shows that transdermal delivery devices can potentially function for diverse healthcare and medical applications while further investigations are still necessary for more efficient skin penetration of gene and drugs.

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Transdermal transport pathway creation: Electroporation pulse order

Cited by 34 publications

References 35 publications

Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses

Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses

Iontophoretic transdermal drug delivery: a multi-layered approach

Recent Advances in Skin Penetration Enhancers for Transdermal Gene and Drug Delivery

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