Transfer of Male or Female Primordial Germ Cells of Quail into Chick Embryonic Gonads.

Ono, Tamao; Yokoi, Ryohei; Aoyama, Hirohiko

doi:10.1538/expanim.45.347

Cited by 49 publications

(37 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Yasuda et al (1992) reported that 63.6% of 100 chick PGCs transferred into quail embryos were present in the gonadal anlage. Furthermore, Ono et al (1996Ono et al ( , 1998aOno et al ( , 1998b reported the migration of chick and quail PGCs following the transfer of embryonic blood containing circulating PGCs. From these studies of the migratory ability of PGCs, it has been postulated that PGCs are chemically attracted by unknown substances released from the developing gonads (Kuwana and Roguska, Journal of Poultry Science, 50 (1) 4 Fig.…”

Section: Conservation Of Avian Genetic Resources Through Germline Chimentioning

confidence: 99%

Conservation of Avian Genetic Resources

Tajima

2013

J. Poult. Sci.

View full text Add to dashboard Cite

Conservation of avian genetic resources is important, as 13.0% (1,313/10,064) of known avian species are currently categorized as endangered by the International Union for Conservation of Nature and Natural Resources (IUCN, 2012). Development of an integrated system for conservation of avian genetics is necessary; however, germplasm conservation systems developed for mammals cannot be applied directly to avians, primarily due to the unique anatomical and physiological characteristics of the avian egg. Thus an alternative strategy for conservation of oviparous species of animals must be developed. Recent technological developments for producing germline chimeras by the transfer of primordial germ cells into recipient embryos has enabled the conservation and retrieval of chicken genetic resources in their complete form. Furthermore, the possibility of propagating endangered wild birds through interspecies germline chimeras has been proposed.In the present review, the current status of avian genetic resource conservation is presented with a focus on the following 4 topics: 1) semen cryopreservation, 2) germline chimeras, 3) somatic nuclear transfer, and 4) other related technologies.

show abstract

Section: Conservation Of Avian Genetic Resources Through Germline Chimentioning

confidence: 99%

Conservation of Avian Genetic Resources

Tajima

2013

J. Poult. Sci.

View full text Add to dashboard Cite

show abstract

“…Chimeric birds have also been produced by PGCs transfer from quail to chicken embryos (Ono et al 1996;. PGCs are not thought to be involved in determining sex, and the cells usually differentiate to the germ cells of the sex which has already been determined according to the somatic sex.…”

Section: Discussionmentioning

confidence: 99%

“…PGCs circulate in the vascular system of the developing embryo and finally migrate into the germinal ridge (Fujimoto et al 1976;Kuwana 1993), where the cells differentiate into spermatogonia or oogonia. PGC transfer between embryonic blood vessels is a common technique for establishment of germ line chimeric chickens (Naito et al 1994;Ono et al 1996;Yamaguchi et al 2000;Furuta et al 2001), and it has been reported that exogenous genes may be introduced into PGCs to produce transgenic chicken (Naito et al 1998;Inada et al 1997;Eguma et al 1999;. For confirmation that transfer of PGCs is technically possible, quail PGCs have been H. Furuta (&) Á T. Sawada Á K. Nishikawa Á I. Yamamoto Á T. Yoshida Á M. Tanaka Department of Animal Science, Nippon Veterinary and Life Science University, Kyounan-cho 1-7-1, Musashino, Tokyo 180-8602, Japan e-mail: fhiroki@nvlu.ac.jp observed in chicken gonads using immunohistochemistry (Ono et al 1998), and the capability of producing chimeric chickens has been investigated by progeny testing (Naito et al 1994;Furuta et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Transfer of blood containing primordial germ cells between chicken eggs development of embryonic reproductive tract

et al. 2007

View full text Add to dashboard Cite

The present study was carried out to investigate development of recipient chicken embryonic reproductive tracts which are transferred chicken primordial germ cells (PGCs). It is thought that differentiation of PGCs is affected by the gonadal somatic cells. When female PGCs are transferred to male embryos, it is possible that they differentiate to W-spermatogonia. However, the relationship development between PGCs and gonads has not been investigated. At stage 12-15 of incubation of fertilized eggs, donor PGCs, which were taken from the blood vessels of donor embryos, were injected into the blood vessels of recipient embryos. The gonads were removed from embryos that died after 16 days of incubation and from newly hatched chickens and organs were examined for morphological and histological features. The survival rate of the treated embryos was 13.6% for homo-sexual transfer of PGCs (male PGCs to male embryo or female PGCs to female embryo) and 28.9% for hetero-sexual transfer PGCs (male PGCs to female embryo or female PGCs to male embryo) when determined at 15 days of incubation. The gonads of embryos arising from homo-sexual transfer appeared to develop normally.In contrast, embryos derived from hetero-sexual transfer of PGCs had abnormal gonads as assessed by histological observation. These results suggest that hetero-sexual transfer of PGCs may influence gonadal development early-stage embryos.

show abstract

“…Numerous studies have reported the generation of chimeric chickens using PGCs as donors and developing embryos as recipients (Vick et al, 1993a;Naito et al, 1994Naito et al, , 1998Ono et al, 1996Ono et al, , 1998aFuruta et al, 1999Furuta et al, , 2001Furuta et al, , 2007Furuta et al, , 2008Furuta and Fujihara, 1999;Yamaguchi et al, 2000). This study primarily focuses on germline chimeric chickens, the conservation of genetic resources, and the transfer of PGCs, including the introduction of exogenous genes.…”

Section: Introductionmentioning

confidence: 99%

Establishing Germline Chimeric Chickens Using Primordial Germ Cells

Furuta

2012

J. Poult. Sci.

View full text Add to dashboard Cite

Chicken primordial germ cells (PGCs) are used to restore and conserve genetic resources of some endangered birds or flocks of domestic chickens. When PGCs are transferred from a donor to a recipient, they can survive and gain sexual maturity in the recipient chicken. This indicates that PGCs retaining exogenous genes can proliferate and develop in recipient gonads, and proves that transferred PGCs are effective sources for establishing transgenic and germline chimeric chickens. The PGC transfer method has been used to produce germline chimeric chickens and may become popular in the field of reproductive science. Germline chimeric chickens have the potential to reconstruct and reproduce endangered birds, including both domestic and wild species.

show abstract

Transfer of Male or Female Primordial Germ Cells of Quail into Chick Embryonic Gonads.

Cited by 49 publications

References 20 publications

Conservation of Avian Genetic Resources

Conservation of Avian Genetic Resources

Transfer of blood containing primordial germ cells between chicken eggs development of embryonic reproductive tract

Establishing Germline Chimeric Chickens Using Primordial Germ Cells

Contact Info

Product

Resources

About