Transfer of pig embryos to different uterine sites.

Wallenhorst, S.; Holtz, W.

doi:10.2527/1999.7792327x

Cited by 36 publications

(32 citation statements)

References 15 publications

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“…Pregnancy rates reported in this study were be�er than or comparable to data reported by Reichenbach et al (1993), Galvin et al (1994), Modl (1994), Hazeleger et al (1995a,b), Wallenhorst and Holtz (1995), Gordon (1997), Cuello et al (2002), Fujino et al (2003). Similar results were also found in our previous studies (Říha et al, 1996, 1997aVejnar, 2000, 2003).…”

Section: Discussioncontrasting

confidence: 47%

Comparison of two vitrification methods for cryopreservation of porcine embryos

Řı́ha¹,

Vejnar²

2004

CZECH J ANIM SCI

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The aim of this study was to compare two vitrification methods of porcine perihatching blastocysts with regard to the success of transfer of these embryos to the recipients. Expanded, hatching, or hatched blastocysts were recovered post mortem from superovulated donors in 5.5 to 6.0 days after artificial insemination of donor gilts with homospermic doses. In protocol VS I, the embryos in perihatching developmental stage were equilibrated in a culture medium H-MEMD with 10% v/v of glycerol (1.37M solution of glycerol in medium) for 10 min and placed in a vitrification medium for 1.5 min max. (vitrification medium contained 50% v/v 2M sucrose in tridistilled water, 30% v/v of glycerol, and 20% v/v of foetal calf serum – FCS). Then they were dropped with micropipette and stored in liquid nitrogen vapour. For protocol VS II, we used H-MEMD culture medium supplemented with 20% v/v of FCS, 25% v/v ethylene glycol, and 25% v/v dimethyl sulphoxide (DMSO). Embryos were equilibrated for 10 min in a mixture of the vitrification medium and culture medium (1 : 1), and were kept in the vitrification medium for 1.5 minutes. Then they were dropped with micropipette and stored in liquid nitrogen vapour. Embryos were thawed by immersing the drop with the embryo in H-MEMD culture medium with 0.8M sucrose for 10 minutes. After thawing and washing in the medium with sucrose, all embryos were washed three times in a fresh medium and prepared for transfer. Recipients were synchronized either using Regumate-feeding followed by treatment with PMSG and HCG (gilts) or using piglet weaning (sows – 1st and 2nd parity). Recipients showing standing heat at the time of donor insemination were used for laparoscopic and non-surgical ET on day 5.5–6.0 of the cycle. The fraction of viable embryo vitrified under VS I or VS II protocol was 85% and 80%, compared to 95% in control fresh embryos (P > 0.05). Pregnancy of recipients was 57.3% (5/7), 67.0% (4/6) for VS I or VS II group and 42.7% (10/23) for control (P < 0.001). We can conclude on the basis of our data that both protocols for vitrification yielded similar results and can be used for cryopreservation of porcine embryos.  

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Section: Discussioncontrasting

confidence: 47%

Comparison of two vitrification methods for cryopreservation of porcine embryos

Řı́ha¹,

Vejnar²

2004

CZECH J ANIM SCI

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“…Too deep deposition of embryos can be unfavorable and cause lower number of pregnancies and number of piglets in one litter (Stein-Stefani andHoltz 1987, Wallenhorst andHoltz 1999). This is the reason for statement that the optimal place of pig embryos deposition is the proximal or the medium part of uterine horn (Stein-Stefani andHoltz 1987, Wallenhorst andHoltz 1999). In our study embryos were transferred into one uterine horn because migration of embryos from one uterine horn to the other is known to occur in swine (Dziuk et al 1964).…”

Section: Discussionmentioning

confidence: 99%

“…In our study, embryos were placed at the proximal part of uterine horn in 1/3 of length of uterine horn from an oviduct isthmus. Too deep deposition of embryos can be unfavorable and cause lower number of pregnancies and number of piglets in one litter (Stein-Stefani andHoltz 1987, Wallenhorst andHoltz 1999). This is the reason for statement that the optimal place of pig embryos deposition is the proximal or the medium part of uterine horn (Stein-Stefani andHoltz 1987, Wallenhorst andHoltz 1999).…”

Section: Discussionmentioning

confidence: 99%

Piglets born after intrauterine laparoscopic embryo transfer

Wieczorek¹,

Koseniuk²,

Mandryk³

et al. 2015

Polish Journal of Veterinary Sciences

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The aim of the study was the preliminary development of laparoscopic transfer of embryos to the uterus in the pig, which can become the alternative for more invasive surgical methods. We proposed the original method of embryo transfer. Donors (n = 40) and recipients (n = 15) of embryos were sows of age of 6-8 months. The estrus cycle of both recipients and donors was routinely synchronized. The experimental animals were divided into two groups. In the first group (10 donors and 3 recipients) embryos were transplanted according to the method described earlier and in the second group (30 donors and 12 recipients) embryos were transplanted according to our own proposed method. As the control group, we used 16 sows after insemination (AI). In animals from both experimental groups pregnancy was diagnosed between 28-31 day after transplantation and in the control group between 28-31 day after insemination. All animals were observed during pregnancy and weaning period in pig farm. Embryos at the development stage of 2-4 cell were obtained surgically and cultured in vitro for 4 days. Obtained blastocysts were transferred to donors. The original set of catheters for blastocysts transfer to pig uterus was constructed. Three trocars were placed in abdominal cavity for inserting endoscope and 2 grasps for uterus stabilization. After uterus stabilization, the slide was inserted into abdomen which was used for putting the needle to puncture uterus. Through this needle catheter with embryos was inserted into the uterus cavity. Embryos were placed by injection into lumen of the one uterine horn. From 12 recipients pregnancy was diagnosed in 6 recipients. From 6 litters, 57 piglets were born. We weaned 41 piglets (71.9%). In our study we obtained 50% efficacy, with the mean number of 9.5 alive piglets in litter and 6.8 weaned piglets. The efficacy of developed method of laparoscopic transfer of porcine embryos allows it to be used in routine practice.

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“…στους χοίρους είναι ο μεγάλος αριθμός απογόνων που παράγονται ανά έτος από τις σΰες αναπαραγωγής, τα χαμηλά ποσοστά εξέλιξης των καταψυγμένων εμβρύων καθώς και οι χειρουργικές μέθοδοι που εφαρμόζονται συ νήθως για τη συλλογή και μεταφορά των εμβρύων. Η ανά πτυξη, τελευταία, τεχνικών διατραχηλικής συλλογής και μεταφοράς εμβρύων καθώς και λαπαροσκοπικών τεχνι κών, παρέχει νέες δυνατότητες ευρύτερης εφαρμογής της μεταφοράς εμβρύων στις σΰες σε προγράμματα γενετικής βελτίωσης, in vitro παραγωγής εμβρύων, προκαθορισμού του φΰλου των εμβρύων, κλωνοποίησης και παραγωγής γενετικά τροποποιημένων χοίρων 1,11,12,15 .…”

Section: συζητησηunclassified

Superovulation of prepubertal and young pubertal gilts with PMSG

Anastasiadis

Amiridis

Lymberopoulos

et al. 2018

J Hellenic Vet Med Soc

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The feasibility of application of a standardized protocol for estrous synchronization, superovulation and embryo transfer in young pubertal and pre-pubertal gilts under farm conditions was investigated. Two groups of Large white X Landrace crossbreed gilts were used. Group A (n = 16) consisted of pubertal (>6months old ) animals weighing 100-110 kg while group Β (n=ll) consisted of prepubertal gilts weighing 75-80 kg. Superovulation and estrous synchronization was conducted by intramuscular administration of 1500 IU of PMSG and 750 IU of hCG given 12 and 72 hours after the transfer of the gilts to the service area (hour 0), respectively. At hours 96 and 108 the animals that exhibited estrous behavior (81,3 and 72.7% for groups A and Β respectively) were inseminated with fresh semen from a Large White boar containing 3xl09 progressively moving spermatozoa. Embryo collection took place 48 – 52 hours after the second insemination. No differences were detected in ovarian response, in the number of collected oocytes and in the number of fertilized ova between the two groups 31,5 ±16,9, 25,2±15,8, 17,5±9,9 and 44,6±22,4, 38,5 ±23,2, 21,6 ±9,5 for groups A and Β respectively. The proportion of fertilized ova was significantly higher in group A when compared to the one from group Β (75.7 vs 60.6% for group A and Β respectively, Ρ < 0.05). A negative correlation was also observed between the number of corpora lutea and fertilization rate (P< 0.0005). It is concluded that the present superovulation scheme can be used safely for sufficient embryo production from both prepubertal and pubertal gilts under farm conditions.

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Transfer of pig embryos to different uterine sites.

Cited by 36 publications

References 15 publications

Comparison of two vitrification methods for cryopreservation of porcine embryos

Comparison of two vitrification methods for cryopreservation of porcine embryos

Piglets born after intrauterine laparoscopic embryo transfer

Superovulation of prepubertal and young pubertal gilts with PMSG

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