Transformation of Chromosomal and Plasmid Characters in Staphylococcus aureus

Abstract

Plasmid and probably also chromosomal characters have been genetically transformed in Staphylococcus aureus. Recipient cells show competence throughout the exponential growth phase with a maximum at early times.Recently Nomura et al. (11) reported on induction of hemolysin synthesis by transformation in Staphylococcus aureus. Earlier re-

Antimicrobial resistance and gene transfer in Staphylococcus aureus

“…The transfer of 'naked' DNA either plasmid or chromosomal can be performed in nitro under certain conditions (Lindberg et al 1972;Sjostron et al 1975). The require-ment for a non-physiological calcium concentration (0.1 mol/l) and the short period of competence (when nucleases are absent) will reduce the likelihood of transformation in nature (Lacey 1984).…”

Factors Affecting Competence for Transformation in Staphylococcus aureus

“…The competent cells were washed once in 0.15 M NaCl and then suspended in 0.1 M tris(hydroxymethyl)aminomethane (Tris)-maleate buffer (pH 7.0) containing 0.1 M CaCl2 at a cell density of approximately 109 CFU/ml. In the transformation experiments, 0.9 ml of this cell suspension was mixed with 0.1 ml of DNA solution to give saturating concentration of DNA, i.e., 10 ug/ml (8). After incubation at 30 C for 20 min, the cells were centrifuged and suspended in 1 ml of TSB 155 on July 31, 2020 by guest…”

“…Transformation in Staphylococcus has been described (7,8), and transfection has been used to determine the requirements for competence in this species (19,20). Competence is generally defined as the ability of a bacterial strain to take up biologically active deoxyribonucleic acid (DNA) and undergo transformation.…”

Genetic analysis of the cap5 locus of Staphylococcus aureus

“…General DNA manipulations were performed as described by Sambrook et al [16]. High molecular weight genomic DNA was isolated from S. aureus as described [17]. DNA was introduced into S. aureus by electroporation [18] or by transduction using bacteriophage 85 [19].…”