Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments

Visser, R.G.F.; Jacobsen, E.; Hesseling-Meinders, A.; Schans, M. J.; Witholt, Bernard; Feenstra, W.J.

doi:10.1007/bf00043210

Cited by 96 publications

(48 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Even though several transformations were conducted throughout the year, transformations initiated during the spring were successful. Similar seasonal effect and very low efficiency of transformation was reported earlier (Visser et al 1989). …”

Section: Transformation Of Potato With Atpdc1 Genesupporting

confidence: 89%

“…It should be noted that transformation was successful with only leaf explants in Atlantic compared to stem explants from Snowden and Dakota Pearl and our experiments are continuing with these transgenics. It was reported that variety as well as explants affect transformation efficiency in potato (Dale and Hampson 1995;Visser et al 1989). Even though several transformations were conducted throughout the year, transformations initiated during the spring were successful.…”

Section: Transformation Of Potato With Atpdc1 Genementioning

confidence: 99%

See 1 more Smart Citation

Alleviation of low temperature sweetening in potato by expressing Arabidopsis pyruvate decarboxylase gene and stress-inducible rd29A: A preliminary study

Pinhero

Pazhekattu

Marangoni

et al. 2011

Physiol Mol Biol Plants

View full text Add to dashboard Cite

The acceptability of potatoes for processing chips and French fries is largely dependent on the color of the finished product. Most potato cultivars and varieties stored at temperatures below 9-10°C are subjected to low temperature sweetening (LTS) which result in the production of bitter-tasting, dark colored chips and French fries which are unacceptable to consumers. However, storing tubers at low temperatures (i.e., <10°C) has many advantages such as lowered weight loss during storage, natural control of sprouting, and reduction/elimination of chemical sprout inhibitors. Our earlier research results on LTS suggested a role for pyruvate decarboxylase (PDC) in LTS-tolerance. In the present study, the role of PDC was examined whereby the potato variety Snowden was transformed with Arabidopsis cold-inducible pyruvate decarboxylase gene 1 (AtPDC1) under the control of promoter rd29A. Two transgenic plants were selected and storage studies were conducted on tubers harvested from one of the transgenic lines grown under green house conditions. Transgenic tubers showed higher Agtron chip color score indicating lighter chip and lower reducing sugar and sucrose concentrations compared to the untransformed tubers during the storage periods studied at 12°C and 5°C. These results suggest that overexpression of pyruvate decarboxylase gene resulted in low temperature sweetening tolerance in the transgenic Snowden.

show abstract

Section: Transformation Of Potato With Atpdc1 Genesupporting

confidence: 89%

Section: Transformation Of Potato With Atpdc1 Genementioning

confidence: 99%

Alleviation of low temperature sweetening in potato by expressing Arabidopsis pyruvate decarboxylase gene and stress-inducible rd29A: A preliminary study

Pinhero

Pazhekattu

Marangoni

et al. 2011

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…We compared the activity of GBSSIa with that of GBSSIb from pea controlled by the same promoter in amf . Further controls included the amf mutant, a wild-type diploid line (which was formed by doubling up from the haploid progenitor of the amf line [Visser et al, 1989]; the amf line also is diploid after chromosome doubling of the haploid), and the amf mutant transformed with the genomic clone of potato GBSSI (BW101; van der .…”

Section: Expression Of Gbssia and Gbssib In The Amf Mutant Of Potatomentioning

confidence: 99%

“…Leaves were harvested at the end of the day. Starch also was analyzed from a wild-type diploid line (which was formed by doubling up from the haploid progenitor of the amf line [Visser et al, 1989]; the amf line also is diploid after chromosome doubling of the haploid) and the amf mutant transformed with the genomic clone of potato GBSSI (BW101; van der . Starch was prepared from pea (Pisum sativum) embryos and leaves of an inbred round-seeded line (BC1/9RR) and from the low-amylose (lam) line 503, which makes no GBSSIa protein .…”

Section: Preparation Of Starchmentioning

confidence: 99%

Discrete Forms of Amylose Are Synthesized by Isoforms of GBSSI in Pea[W]

Edwards

Vincken²,

Suurs³

et al. 2002

The Plant Cell

View full text Add to dashboard Cite

Amyloses with distinct molecular masses are found in the starch of pea embryos compared with the starch of pea leaves. In pea embryos, a granule-bound starch synthase protein (GBSSIa) is required for the synthesis of a significant portion of the amylose. However, this protein seems to be insignificant in the synthesis of amylose in pea leaves. cDNA clones encoding a second isoform of GBSSI, GBSSIb, have been isolated from pea leaves. Comparison of GBSSIa and GBSSIb activities shows them to have distinct properties. These differences have been confirmed by the expression of GBSSIa and GBSSIb in the amylose-free mutant of potato. GBSSIa and GBSSIb make distinct forms of amylose that differ in their molecular mass. These differences in product specificity, coupled with differences in the tissues in which GBSSIa and GBSSIb are most active, explain the distinct forms of amylose found in different tissues of pea. The shorter form of amylose formed by GBSSIa confers less susceptibility to the retrogradation of starch pastes than the amylose formed by GBSSIb. The product specificity of GBSSIa could provide beneficial attributes to starches for food and nonfood uses.

show abstract

“…Russet Burbank com o gene da capa protéica do vírus X e Y. Os explantes produziram brotações com uma freqüência de 60% na ausên-cia de seleção e de 10% em meio com 100 mg.L -1 de can. Visser et al (1989) analisaram brotações de batata regeneradas em meio com can, sendo que mais de 90% dessas eram transformadas. Trinca et al (1990) também observaram que a presença de can no meio de regeneração foi eficiente na produção de até 100% de brotações de batata cv.…”

Section: Resultsunclassified

Transformação genética da batata cultivar Achat via Agrobacterium tumefaciens

et al. 2000

View full text Add to dashboard Cite

O melhoramento genético tradicional da batata (Solanum tuberosum L.) é lento e defronta-se com uma gama de dificuldades. A espécie cultivada é tetraplóide com segregação polissômica, e muitas das cultivares comerciais apresentam baixa fertilidade de pólen ou não florescem (Vayda et al., 1992). Nesse cenário, a utilização das técnicas de transformação genética em batata teria a vantagem de possibilitar a incorporação, em clones considerados elites, de genes específicos que codificam características de interesse. Achat é uma das cultivares lideres no mercado nacional, no entanto, reduções nos níveis de produtividade desse genótipo têm sido reportados devido a infecção por viroses do grupo potivirus e luteovirus.Estratégias de engenharia genética usando genes derivados de patógenos têm sido empregadas com sucesso no controle de doenças virais em diversas culturas incluindo a batata (Scholthof et al., 1993), permitindo o desenvolvimento, a curto prazo, de cultivares melhora- ABSTRACT Genetic transformation of potato cultivar Achat by Agrobacterium tumefaciens.Nodal segment explants of potato (Solanum tuberosum L.) cv Achat were excised from in vitro growing plants, and transformed with Agrobacterium tumefaciens LBA4404 carrying the binary vector pBI121 with npt II and gus-intron genes. The regeneration of putative transformants was done in medium supplemented with 50 mg.L -1 of kanamycin. Putative transformants expressing kanamycin resistance and b-glucuronidase activity were identified. PCR analysis of these plants showed amplification of the npt II in gus + plants. Southern blotting hybridization revealed the insertion of at least one copy of the npt II gene.

show abstract

Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments

Cited by 96 publications

References 32 publications

Alleviation of low temperature sweetening in potato by expressing Arabidopsis pyruvate decarboxylase gene and stress-inducible rd29A: A preliminary study

Alleviation of low temperature sweetening in potato by expressing Arabidopsis pyruvate decarboxylase gene and stress-inducible rd29A: A preliminary study

Discrete Forms of Amylose Are Synthesized by Isoforms of GBSSI in Pea[W]

Transformação genética da batata cultivar Achat via Agrobacterium tumefaciens

Contact Info

Product

Resources

About