Transformation of intact yeast cells treated with alkali cations or thiol compounds.

Ito, Hisao; Mutata, Kousaku; Kimura, Akira

doi:10.1271/bbb1961.48.341

Cited by 1,419 publications

(1,496 citation statements)

References 0 publications

Supporting

Mentioning

1,491

Contrasting

Unclassified

Order By: Relevance

“…Rich YPD (2% glucose, 2% polypeptone, and 1% yeast extract) and minimal EMM2 (Mitchison 1970) were employed. Transformation of S. pombe was performed by the lithium method (Ito et al 1983). Temperature sensitive mis3-224 strain was grown in YPD to 2´10 6 cells per mL at 26 8C and then shifted to 36 8C.…”

Section: Strains Genetical Methods and Macromolecular Analysismentioning

confidence: 99%

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

2000

View full text Add to dashboard Cite

show abstract

Section: Strains Genetical Methods and Macromolecular Analysismentioning

confidence: 99%

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

2000

View full text Add to dashboard Cite

show abstract

“…Solid media contained 2% agar (Difco). Transformation of yeast strains was performed by the lithium acetate method (Ito et al, 1983).…”

Section: Yeast Strains Media and Transformationmentioning

confidence: 99%

TCA cycle‐independent acetate metabolism via the glyoxylate cycle in Saccharomyces cerevisiae

Lee

Jang

Kim

et al. 2010

Yeast

View full text Add to dashboard Cite

In Saccharomyces cerevisiae, the accepted theory is that due to TCA cycle dysfunction, the cit1 mutant lacking the mitochondrial enzyme citrate synthase (Cit1) cannot grow on acetate, regardless of the presence of the peroxisomal isoenzyme (Cit2). In this study, we re-evaluated the roles of Cit1 and Cit2 in acetate utilization and examined the pathway of acetate metabolism by analysing mutants defective in TCA or glyoxylate cycle enzymes. Although cit1 cells showed significantly reduced growth on rich acetate medium (YPA), they exhibited growth similar to cit2 and the wild-type cells on minimal acetate medium (YNBA). Impaired acetate utilization by cit1 cit2 cells on YNBA was restored by ectopic expression of either Cit2 or its cytoplasmically localized variants. Deletion of any of the genes for the enzymes solely involved in the TCA cycle (IDH1, KGD1 and LSC1 ), except for SDH1, caused little defect in acetate utilization on YNBA but resulted in significant growth impairment on YPA. In contrast, cells lacking any of the genes involved in the glyoxylate cycle (ACO1, FUM1, MLS1, ICL1 and MDH2 ) did not grow on either YNBA or YPA. Deletion of SFC1 encoding the succinate-fumarate carrier also caused similar growth defects on YNBA. Our results suggest that in S. cerevisiae the glyoxylate cycle functions as a competent metabolic pathway for acetate utilization on YNBA, while both the TCA and glyoxylate cycles are essential for growth on YPA.

show abstract

“…For the culture of S. pombe, the rich YPD and minimal EMM2 media were used (Mitchison 1970). The lithium acetate method (Ito et al 1983) was used for transformation of S. pombe cells.…”

Section: Strains Transformation and Mediamentioning

confidence: 99%

Dissection of fission yeast microtubule associating protein p93^Dis1: regions implicated in regulated localization and microtubule interaction

et al. 1996

View full text Add to dashboard Cite

Background: Fission yeast microtubule associating protein (MAP) p93 Dis1 functions for sister chromatid separation: dis1 mutants fail to separate chromosomes, while the spindle elongates but without cyclin destruction. p93 Dis1 localizes along microtubules in interphase cytoplasm, but shifts to the spindle pole body (SPB) and spindle microtubules upon the entry into mitosis. In this study, regions of p93 Dis1 were dissected to examine their role.

show abstract

Transformation of intact yeast cells treated with alkali cations or thiol compounds.

Cited by 1,419 publications

References 0 publications

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

TCA cycle‐independent acetate metabolism via the glyoxylate cycle in Saccharomyces cerevisiae

Dissection of fission yeast microtubule associating protein p93^Dis1: regions implicated in regulated localization and microtubule interaction

Contact Info

Product

Resources

About

Transformation of intact yeast cells treated with alkali cations or thiol compounds.

Cited by 1,419 publications

References 0 publications

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

TCA cycle‐independent acetate metabolism via the glyoxylate cycle in Saccharomyces cerevisiae

Dissection of fission yeast microtubule associating protein p93Dis1: regions implicated in regulated localization and microtubule interaction

Contact Info

Product

Resources

About

Dissection of fission yeast microtubule associating protein p93^Dis1: regions implicated in regulated localization and microtubule interaction