Transforming growth factor type e is a novel mediator of wound repair

Carter, Bobbie J.; Halper, Jaroslava

doi:10.1046/j.1524-475x.1996.40215.x

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…Materials. Two strains of Lactobacillus acidophilus (ATCC strains 4356 and 43121) were plated (l:1) in MRS broth (pH 5.5, Difco Laboratories, Detroit, MI) and co- (19,20). LS suspended in 2% methylcellulose (40 /lol of LS cultured at 37°C for 24 hrs under microaerophilic condi-in 50 /lol 2% methylcellulose in PBS) was applied to the tions, The MRS culture medium contains polysorbate, ac-wound at the time of biopsy.…”

Section: Methodsmentioning

confidence: 99%

Wound Healing and Angiogenic Properties of Supernatants from Lactobacillus Cultures

Halper¹,

Leshin

Lewis

et al. 2003

Exp Biol Med (Maywood)

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Extracts or supernatants from cultures of Lactobacilli are used for their medicinal effects, including wound healing and immune system stimulating activity. We have studied the in vivo and in vitro effects of supernatants from bacterial cultures of two strains of Lactobacillus (LS) on tissue repair and angiogenesis. Subcutaneous injection of LS into rodent ears led to proliferation of blood vessels that also exhibited strong immunostaining for Flk-1 receptor. Some inflammatory cells were scattered among the blood vessels. The continuous influx of polymorphonuclear leukocytes (PMNs) and macrophages into transcutaneous wounds in mice treated with LS resulted in prolonged inflammatory phase of wound healing and delayed wound closure, including reepithelialization. Subcutaneous injection of Matrigel impregnated with LS into the abdominal wall led to rapid and transient influx of PMNs in the vicinity of the gel. LS stimulated the proliferation of murine macrophage J774.A1 cell line and porcine lymphocytes but not that of murine fibroblast AKR-2B cells. LS also induced production of TNF-alpha by J774.A1 cells and by porcine kidney epithelial LLC-PK1 cells. LS did not appear to have an effect on collagen production. In conclusion, our study demonstrates the potential of LS to function as a stimulator of the inflammatory stage of tissue repair, TNF-alpha production, and of angiogenesis.

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Section: Methodsmentioning

confidence: 99%

Wound Healing and Angiogenic Properties of Supernatants from Lactobacillus Cultures

Halper¹,

Leshin

Lewis

et al. 2003

Exp Biol Med (Maywood)

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Progranulin is a mediator of the wound response

Ong

Halper

et al. 2003

Nat Med

444

395

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Annually, 1.25 million individuals suffer burns in the United States and 6.5 million experience chronic skin ulcers, often from diabetes, pressure or venous stasis. Growth factors are essential mediators of wound repair, but their success as therapeutics in wound treatment has, so far, been limited. Therefore, there is a need to identify new wound-response regulatory factors, but few have appeared in recent years. Progranulin (also called granulin or epithelin precursor, acrogranin or PC-derived growth factor) is a growth factor involved in tumorigenesis and development. Peptides derived from progranulin have been isolated from inflammatory cells, which led to suggestions that progranulin gene products are involved in the wound response, but this remains undemonstrated. We report that in murine transcutaneous puncture wounds, progranulin mRNA is expressed in the inflammatory infiltrate and is highly induced in dermal fibroblasts and endothelia following injury. When applied to a cutaneous wound, progranulin increased the accumulation of neutrophils, macrophages, blood vessels and fibroblasts in the wound. It acts directly on isolated dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures. Progranulin is, therefore, a probable wound-related growth factor.

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Culture Supernatant of Lactobacillus acidophilus Stimulates Proliferation of Embryonic Cells

Brackett²,

Halper

2005

Exp Biol Med (Maywood)

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Our previous report showed that supernatants of Lactobacillus acidophilus (LS) cultures possessed chemotactic and angiogenic properties. Specifically, LS stimulated gene expression and the secretion of tumor necrosis factor-alpha (TNF-alpha), the proliferation of immune cells in vitro, and blood vessel formation. Chemotaxis and proliferation of inflammatory cells in vivo were also stimulated by LS. In the current study, we hypothesized that LS stimulates the growth and development of other rapidly dividing cells, including embryonic cells. The stimulatory effects of LS on a neuroblastoma cell line (Neuro-2a), chicken embryos, and bovine embryos were examined. The addition of LS to Neuro-2a cultures caused a proliferation of cells in a concentration-dependent manner. Pretreatment of LS at 56 degrees C for 30 mins did not affect its stimulatory activity. The administration of LS to the chorioallantoic membrane (CAM) of chicken-embryonated eggs for 1-2 days resulted in extensive thickening of the membrane. The thickening was due to the influx and proliferation of fibroblasts and inflammatory cells, the accumulation of loose connective tissue composed primarily of mucopolysaccharides, and/or the formation of blood vessels. Stimulatory effects of LS on bovine embryos were also observed. The treatment with LS significantly promoted the development of zygotes to the four-cell stage and from the four-cell stage to blastocysts. These results have confirmed our hypothesis that LS exerts a stimulatory effect on the cells of embryonic stages including neuroblastoma cells, the CAM of chicken embryos, and bovine embryos from zygotes to blastocysts.

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Transforming growth factor type e is a novel mediator of wound repair

Cited by 3 publications

References 11 publications

Wound Healing and Angiogenic Properties of Supernatants from Lactobacillus Cultures

Wound Healing and Angiogenic Properties of Supernatants from Lactobacillus Cultures

Progranulin is a mediator of the wound response

Culture Supernatant of Lactobacillus acidophilus Stimulates Proliferation of Embryonic Cells

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