Transgenic Approaches in Plants: Strategic Control for Disease Management

Ijaz, Siddra; Babar, Maria; Razzaq, Hafiza Arooj; Nasir, Bukhtawer

doi:10.1007/978-3-030-35955-3_9

Cited by 2 publications

(2 citation statements)

References 168 publications

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“…Despite a long history of pathological research studies on D. sissoo dieback, the infection persists to this day in D. sissoo plantations with a varying frequency of incidence and severity. This indicates that further research is needed to explore the pathological cause of the disease by employing conventional and molecular pathological techniques ( Ijaz et al, 2020 ). Numerous studies attempted to elucidate the biology of D. sissoo dieback ( Hassan et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Phylogenomics resolves the etiology of dieback disease and deciphers Ceratocystis dalbergicans sp.nov., causal agent of Dalbergia sissoo decline

Haq

Ijaz²,

Latif³

et al. 2023

Front. Genet.

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Dalbergia sissoo is one of the most economically important trees in forestry, agroforestry, and horticulture. This tree species is severely threatened by dieback. Widespread dieback outbreaks and infestations have drastically destroyed billions of D. sissoo trees. Hence, we attempted to resolve the dieback etiology through phylogenomics associated with D. sissoo mortality. The Ceratocystis species was evaluated using morphologically investigated fungal isolates collected from dieback-affected tissue plants. Based on the symptomatology, we have differentiated dieback from Fusarium wilt and concluded that the Ceratocystis fimbriata sensu lato complex is causing shisham dieback in Pakistan. As the Ceratocystis species complex is a cryptic species complex, we used genomics and phylogenetic analysis for deciphering its evolutionary hierarchical order. The pathogen’s operational taxonomy was unlocked with the help of phylogenomics, and it was discovered that isolates from D. sissoo represent a species distinct from the other species in the C. fimbriata sensu lato species complex. The name Ceratocystis dalbergicans sp. nov. has been given to the fungus causing dieback disease in D. sissoo.

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Section: Introductionmentioning

confidence: 99%

Phylogenomics resolves the etiology of dieback disease and deciphers Ceratocystis dalbergicans sp.nov., causal agent of Dalbergia sissoo decline

Haq

Ijaz²,

Latif³

et al. 2023

Front. Genet.

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“…For this purpose, model species have been extensively studied for insight into infection stages and host interactions (Haq and Ijaz, 2020). Different techniques are involved in expression profiling, including northern blotting, semiquantitative real-time PCR, microarray, RNAseq, and quantitative real-time PCR (qRT−PCR) (Ijaz et al, 2020a). Among these, qRT-PCR is the most efficient technique for determining the relative levels of target gene expression in different samples (MaChado et al, 2015;Ijaz et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Selection and validation of reference genes for normalizing qRT‒PCR gene expression studies in Colletotrichum gloeosporioides and interaction with the guava plants

Haq,

Ijaz,

Hashem

et al. 2023

Front. Plant Sci.

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Quantitative real-time PCR is used to quantify gene expression, even to detect low-level transcripts. It detects and quantifies the inoculum level of fungal pathogens in infected hosts. However, reliable expression profiling data require accurate transcript normalization against a stable reference gene. Hence, using stably expressed reference genes under variable conditions is paramount in gene expression analysis. In the current study, reference genes were selected and validated in Colletotrichum gloeosporioides, a guava canker and dieback pathogen. The reference gene selection and validation in C. gloeosporioides were evaluated for germinated conidia and mycelium (in vitro) and in infected guava (Psidium guajava) (interaction with host plant). The CgCAL gene was determined as a highly stable reference gene, followed by the CgTUB2 in C. gloeosporioides for germinating conidia and mycelium. However, the CgTUB2 gene was determined to be a highly stable reference gene, followed by the CgCAL for expression analysis during its interaction with the plant. Expression profiling revealed stable and constant relative expression patterns of selected reference genes for both PR genes by determining their relative transcript level. This study is the first to describe reference gene selection and validation to quantify target gene expression in C. gloeosporioides.

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Transgenic Approaches in Plants: Strategic Control for Disease Management

Cited by 2 publications

References 168 publications

Phylogenomics resolves the etiology of dieback disease and deciphers Ceratocystis dalbergicans sp.nov., causal agent of Dalbergia sissoo decline

Phylogenomics resolves the etiology of dieback disease and deciphers Ceratocystis dalbergicans sp.nov., causal agent of Dalbergia sissoo decline

Selection and validation of reference genes for normalizing qRT‒PCR gene expression studies in Colletotrichum gloeosporioides and interaction with the guava plants

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