Transgenic basmati rice transformed with the Xa21 gene shows resistance against bacterial leaf blight

Abstract

Rice (Oryza sativa L.) cultivar Super Basmati was transformed with a cloned gene, Xa21, through particle bombardment method. Twenty-two plants were recovered from the selected calli. Initial screening through PCR confirmed the presence of the transgene in 21 plants. Southern analysis further confirmed the integration of the Xa21 gene into the genome of these transgenic plants. Analysis of the T 1 generation revealed that both the hpt and Xa21 genes were successfully transmitted from the primary transformants to the progeny. Bioassays with two local isolates of bacterial leaf blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo), showed that 14 transgenic lines were resistant to both Xoo isolates. No significant differences were observed in yield and grain characteristics between transgenic lines and the control; however, the transgenic lines took 8-12 days longer to flower and showed reduced plant height compared to the control plants. In conclusion, we have developed bacterial blight-resistant transgenic lines that can be further exploited by breeders for rice improvement.

Genetiği Değiştirilmiş Bitkilerin Biyogüvenlik Riskleri

“…Çeşitli biyotik ve abiyotik stres faktörleri ile mücadele etmeye yönelik gen aktarım çalışmaları yapılmıştır [11,13,14]. Biyotik stres faktörlerine karşı yapılan çalışmalar çoğunlukla herbisitlere [15,16], böceklere [17][18][19], virüslere ve bakterilere [20][21][22][23] karşı dirençli transgenik bitkiler elde etmeye yönelik olmuşken, abiyotik stres faktörlerine karşı dirençli transgenik ürün çalışmaları kuraklık [24] ve tuzluluk [25,26] gibi stres faktörlerine yönelik yapılmıştır. Bunlarla beraber gen susturma yöntemleri [27] bir çözüm bulunması henüz mümkün olmamıştır [28,29].…”

Resistance gene expression in selected Indonesian pigmented rice varieties against infection by Xanthomonas oryzae pv. oryzae

“…This technique is widely used to promote the shar ing of beneficial genetic resources between germplasms, especially those conferring resistance against BLB disease resistance. This beneficial genetic resource sharing is ac complished through molecular breeding, such as molec ular assisted selection and genetic engineering (Chukwu et al 2019; Mukhtar andHasnain 2017). Indonesia pos sesses over 17,000 rice germplasms, including wild and pigmented rice (Maulana et al 2014; Mau et al 2017, demonstrating diverse morphology, biochemical proper ties, and genetic characteristics.…”