Transgenic Oil Palm with Stably Integrated CpTI Gene Confers Resistance to Bagworm Larvae

Abdullah, Rohana; Chari, Christine; Ping, Winnie Yap Soo; Huey, Yeun Li

doi:10.1007/978-94-017-2679-5_29

Cited by 8 publications

(6 citation statements)

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“…DNA was isolated according to Abdullah et al (2003) and was later coated on gold particles described by Sanford et al (1993). pBI 121 (Clontech) containing neomycin phosphotransferase (NPT II) and b-glucuronidase (GUS) genes were used in all initial transformation experiment.…”

Section: Genetic Transformation Assessment Of Ies Using Biolisticmentioning

confidence: 99%

“…Histochemical assays were performed according to protocols described by Abdullah et al (2003). Assays were carried out on IEs, germinating IE, callus and tissue from plantlets 7 days, 3, 9 and 18 months after co-cultivation.…”

Section: Analyses Of Putative Transformed Ies Following Co-mentioning

confidence: 99%

“…However, these reports lack substantiated evidence for stable integration of transgenes transferred (Abdullah et al 2003). Here, we report the potential role of immature embryos (IEs) in genetic transformation studies of oil palm.…”

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confidence: 91%

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Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

Abdullah¹,

Zainal²,

Heng³

et al. 2005

Electro Journal of Biotech

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Section: Genetic Transformation Assessment Of Ies Using Biolisticmentioning

confidence: 99%

Section: Analyses Of Putative Transformed Ies Following Co-mentioning

confidence: 99%

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Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

Abdullah¹,

Zainal²,

Heng³

et al. 2005

Electro Journal of Biotech

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“…Current conventional breeding programmes for oil palm are supported by modern biotechnology approaches such as the use of tissue culture, genetic transformation and marker-aided selection, to further improve oil palm commercial value and productivity (Mayes et al 2000). Over the past 10 years, our group has been actively involved in genetic engineering of oil palm (Abdullah et al 2003;Abdullah et al 2005). Although many studies have been done, but there are very few publications on genetic manipulation of oil palm, probably due to the high commercial implication tagged to the crop and also due to the crops own physiological characteristics such as long generation cycle.…”

mentioning

confidence: 99%

“…Special emphasis was placed initially in developing techniques for genes transfer into oil palm, both using direct gene transfer (DGT) and Agrobacteriummediated approaches. Both techniques were then used to transfer useful genes such as cowpea trypsin inhibitor (CpTI) (Abdullah et al 2003) and Bacillus thuringiensis (Bt) crystal insecticidal protein genes, to address problems related to insect pests (Sharma et al 2000;Sharma et al 2002) and chitinase (to address problems related to basal stem rot).…”

mentioning

confidence: 99%

Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm

Lee¹,

Yeun²,

Abdullah³

2006

Electron. J. Biotechnol.

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Oil palm, like all other crops, is susceptible to attack from several insect pests causing significant reduction in productivity. In the past, cry genes from Bacillus thuringiensis have been reported to be effective in conferring resistance towards insect pests in crops such as corn and rice. One of the advantages of these toxin proteins is their specificity towards certain harmful insects. A rapid and efficient method was developed for the transformation and evaluation of CryIA(b) expression in oil palm. A recombinant vector was introduced into immature embryos (IEs) of oil palm via the biolistic method. More than 700 putative transformed IEs from independent transformation events were generated. Transient transformation efficiency of 81-100 % was achieved. We found that pretreatment of target tissues with phytohormones is essential for increasing the transformation efficiency. This finding could enable higher transformation rate in

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Cowpea (Vigna unguiculata L. Walp), a renewed multipurpose crop for a more sustainable agri‐food system: nutritional advantages and constraints

et al. 2016

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The growing awareness of the relevance of food composition for human health has increased the interest of the inclusion of high proportions of fruits and vegetables in diets. To reach the objective of more balanced diets, an increased consumption of legumes, which constitutes a sustainable source of essential nutrients, particularly low-cost protein, is of special relevance. However, the consumption of legumes also entails some constraints that need to be addressed to avoid a deleterious impact on consumers' wellbeing and health. The value of legumes as a source of nutrients depends on a plethora of factors, including genetic characteristics, agro-climatic conditions, and postharvest management that modulate the dietary effect of edible seeds and vegetative material. Thus, more comprehensive information regarding composition, especially their nutritional and anti-nutritional compounds, digestibility, and alternative processing procedures is essential. These were the challenges to write this review, which focusses on the nutritional and anti-nutritional composition of Vigna unguiculata L. Walp, an emerging crop all over the world intended to provide a rational support for the development of valuable foods and feeds of increased commercial value. © 2016 Society of Chemical Industry.

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Transgenic Oil Palm with Stably Integrated CpTI Gene Confers Resistance to Bagworm Larvae

Cited by 8 publications

References 2 publications

Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm

Cowpea (Vigna unguiculata L. Walp), a renewed multipurpose crop for a more sustainable agri‐food system: nutritional advantages and constraints

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