Transient behavior of a continuous stirred tank biological reactor utilizing phenol as an inhibitory substrate

Abstract

SummaryTransient experiments were conducted on a Pseudmomas utilizing phenol in a continuous culture by disturbing the influent substrate concentration and dilution rate. Two stable steady states existed for some ranges of the parameters. Highly damped oscillations were observed in approaching a new high conversion steady state or in returning to a new high conversion steady state following a small disturbance. When a large disturbance was applied there was a smooth (overdamped) approach to a new low conversion steady state.The observed oscillatory behavior for small disturbances was predicted by a modified Powell-Ierusalemskii bottleneck model, but could not be predicted by a Monod-Haldane model; neither model was accurate for predicting the effect of large disturbances.A constant wall growth factor was used to account for microbial film activity, and the existence of two stable steady states was directly due to the presence of the film.

Effect of temperature on the inhibition kinetics of phenol biodegradation byPseudomonas putida Q5

“…On the other hand, there was much less variability in Y X/S in continuous cultures, possibly because the range of steady-state substrate concentrations was very narrow. The ranges of values for Y X/S determined from batch and average values for continuous cultures in this work are consistent with those reported in the literature for various strains of P. putida (0.52-0.78 g dry weight/g phenol) (Chi and Howell, 1976;Hill and Robinson, 1975;Hutchinson and Robinson, 1988).…”

“…The parameter estimates at 25°C are similar to those estimated for mesophiles at 30°C, although it is surprising that the µ max for two strains of mesophilic P. putida (Chi and Howell, 1976;Hutchinson and Robinson, 1988) at 30°C was lower than the value obtained for psychrotrophic P. putida Q5 at 25°C. The parameter estimates at 10°C for µ max , K S , and K I are different from those of Kotturi et al (1991) using the same strain of P. putida at the same temperature.…”

Kinetics of high strength phenol degradation using Bacillus brevis

“…The biodegradation of phenol and its derivatives by microbial cultures has been the focus of research for more than three decades [8,[16][17][18][19][20]. These studies have been conducted with different micro-flora, under varied environmental conditions of pH, temperature, addition of co-substrate, in the presence or absence of oxygen, in batch and continuous flow reactors.…”

Phenol biodegradation by a microbial consortium: application of artificial neural network (ANN) modelling

“…For pure cultures, a variety of kinetic substrate inhibition models have been used to describe the dynamics of microbial growth on phenol. Of these various inhibition models, the Andrews equation has been used extensively to describe phenol biodegradation [6][7][8][9]. Information on the kinetics of phenol biodegradation will improve process control and the efficiency of phenol treatment or removal at wastewater treatment plants.…”