Transient concentrations of NaCl affect the PHA accumulation in mixed microbial culture

Palmeiro-Sánchez, Tania; Fra-Vázquez, Andrea; Rey-Martínez, Natalia; Campos, J. L.; Mosquera-Corral, Anuska

doi:10.1016/j.jhazmat.2015.12.032

Cited by 29 publications

(22 citation statements)

References 25 publications

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“…Enrichment of a halophilic PHA accumulating consortium under ADF conditions has been recently investigated using different carbon sources as substrate, which resulted in cellular PHA contents reaching up to 65% and 61% PHA using acetate and glucose respectively, demonstrating the potential of this strategy in MMC [ 117 ]. Recently, a previously enriched MMC fed with a mixture of VFAs containing 0.8 g/L Na + was examined regarding its PHA accumulation capacity under transient concentrations of 7, 13, and 20 g/L NaCl [ 118 ]. Since the particular MMC was not adapted to saline conditions, PHA accumulation capacities and rates decreased with higher NaCl concentrations while biopolymer composition was affected in terms of HB:HV ratio.…”

Section: Pha Production By Mixed Microbial Consortia (Mmc)mentioning

confidence: 99%

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

et al. 2017

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Sustainable biofuels, biomaterials, and fine chemicals production is a critical matter that research teams around the globe are focusing on nowadays. Polyhydroxyalkanoates represent one of the biomaterials of the future due to their physicochemical properties, biodegradability, and biocompatibility. Designing efficient and economic bioprocesses, combined with the respective social and environmental benefits, has brought together scientists from different backgrounds highlighting the multidisciplinary character of such a venture. In the current review, challenges and opportunities regarding polyhydroxyalkanoate production are presented and discussed, covering key steps of their overall production process by applying pure and mixed culture biotechnology, from raw bioprocess development to downstream processing.

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Section: Pha Production By Mixed Microbial Consortia (Mmc)mentioning

confidence: 99%

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

et al. 2017

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“…This means a feast of about 21% of the total time of the cycle. The enrichment showed a stable performance despite the fact that ammonium was present in relatively high concentration in comparison with other conventional enrichment configurations [ 27 , 30 , 39 ].…”

Section: Resultsmentioning

confidence: 99%

“…However, unlike the fish-canning streams, these effluents are characterised by the presence of carbohydrates—and lipids in the lesser cases—and the absence of significant amounts of proteins or sodium chloride, which are a typical characteristic of fish-canning wastewater. On the one hand, salinity is known to affect the PHA accumulation capacity [ 27 ] and the properties of the obtained biopolymer [ 28 ] in mixed microbial cultures. On the other hand, relevant concentrations of proteins lead to the presence of considerable amounts of ammonium in the medium of reaction.…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of Organic Pollutants in Fish-Canning Wastewater into Volatile Fatty Acids and Polyhydroxyalkanoate

Palmeiro-Sánchez

Campos

Mosquera-Corral

2021

IJERPH

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The wastewater from the cookers of a tuna-canning plant was used as feedstock for the process. It was acidified in a continuous stirred tank reactor (CSTR) of 1.5 L to produce a mixture of volatile fatty acids (VFAs). The effluent contained 28.3 ± 8.7 g CODS/L and 25.0 ± 4.6 g CODVFA/L, 4.4 ± 1.6 g NH4+/L, and 10.9 ± 4.0 g Na+/L, which corresponds to about 28 g NaCl/L approximately. This was used to feed a PHA production system. The enriched MMC presented a capacity to accumulate PHAs from the fermented tuna wastewater. The maximum PHA content of the biomass in the fed-batch (8.35 wt% PHA) seemed very low, possibly due to the variable salinity (from 2.2 up to 12.3 g NaCl/L) and the presence of ammonium (which promoted the biomass growth). The batch assay showed a PHA accumulation of 5.70 wt% PHA, but this is a much better result if the productivity of the reactor is taken into account. The fed-batch reactor had a productivity of 10.3 mg PHA/(L h), while the batch value was about five times higher (55.4 mg PHA/(L h)). At the sight of the results, it can be seen that the acidification of fish-canning wastewater is possible even at high saline concentrations (27.7 g NaCl/L). On the other hand, the enrichment and accumulation results show us promising news and which direction has to be followed: PHAs can be obtained from challenging substrates, and the feeding mode during the accumulation stage has an important role to play when it comes to inhibition.

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“…Palmeiro-Sánchez et al, explored how imposing recurrent sodium chloride concentrations (NaOH, 0.8 g Na + /L) would influence the resulting MMC cultures ability to produce PHA. Enrichments were on a mixture of C2-C5 aliphatic VFAs where the major constituents were acetic and propionic acids (54 and 27 mol%, respectively) [ 191 ]. PHA production by the MMC reached 53% of the CDW and the corresponding copolymer composition was P(3HB- co -27 mol%3HV) [ 191 ].…”

Section: Pha Production Using Mixed Culturesmentioning

confidence: 99%

“…Enrichments were on a mixture of C2-C5 aliphatic VFAs where the major constituents were acetic and propionic acids (54 and 27 mol%, respectively) [ 191 ]. PHA production by the MMC reached 53% of the CDW and the corresponding copolymer composition was P(3HB- co -27 mol%3HV) [ 191 ]. A comparative study where the NaOH was not imposed during enrichments resulted in a relatively lower ability of the resulting MMC to produce PHA.…”

Section: Pha Production Using Mixed Culturesmentioning

confidence: 99%

Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production

2021

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Petroleum-derived plastics dominate currently used plastic materials. These plastics are derived from finite fossil carbon sources and were not designed for recycling or biodegradation. With the ever-increasing quantities of plastic wastes entering landfills and polluting our environment, there is an urgent need for fundamental change. One component to that change is developing cost-effective plastics derived from readily renewable resources that offer chemical or biological recycling and can be designed to have properties that not only allow the replacement of current plastics but also offer new application opportunities. Polyhydroxyalkanoates (PHAs) remain a promising candidate for commodity bioplastic production, despite the many decades of efforts by academicians and industrial scientists that have not yet achieved that goal. This article focuses on defining obstacles and solutions to overcome cost-performance metrics that are not sufficiently competitive with current commodity thermoplastics. To that end, this review describes various process innovations that build on fed-batch and semi-continuous modes of operation as well as methods that lead to high cell density cultivations. Also, we discuss work to move from costly to lower cost substrates such as lignocellulose-derived hydrolysates, metabolic engineering of organisms that provide higher substrate conversion rates, the potential of halophiles to provide low-cost platforms in non-sterile environments for PHA formation, and work that uses mixed culture strategies to overcome obstacles of using waste substrates. We also describe historical problems and potential solutions to downstream processing for PHA isolation that, along with feedstock costs, have been an Achilles heel towards the realization of cost-efficient processes. Finally, future directions for efficient PHA production and relevant structural variations are discussed.

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Transient concentrations of NaCl affect the PHA accumulation in mixed microbial culture

Cited by 29 publications

References 25 publications

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

Bioconversion of Organic Pollutants in Fish-Canning Wastewater into Volatile Fatty Acids and Polyhydroxyalkanoate

Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production

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