Transient expression of neurofilament protein during hair cell development in the mouse cochlea

Hasko, J.A; Richardson, G. P.; Russell, Ian J.; Shaw, Gerry

doi:10.1016/0378-5955(90)90183-p

Cited by 20 publications

(12 citation statements)

References 14 publications

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“…Ctbp2 and Bassoon mark presynaptic ribbons of hair cells (tom Dieck et al, 2005; Khimich et al, 2005; Kujawa and Liberman, 2009) and GluR2/3 marks postsynaptic densities on the neurons (Liberman et al, 2011). Odf2 is most similar to Neurofilament, which is expressed in spiral ganglion neurons both pre- and post-natally, and is also transiently expressed in hair cells shortly after birth (Hasko et al, 1990). …”

Section: Discussionmentioning

confidence: 99%

Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti

Carlisle

Steel

Lewis

2012

Gene Expression Patterns

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Section: Discussionmentioning

confidence: 99%

Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti

Carlisle

Steel

Lewis

2012

Gene Expression Patterns

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“…Recently Hasko et al [20] discovered a transient expression of NFs also in cochlear hair cells of the developing mouse, by using polyclonal antibodies. This observation, in contrast to earlier studies when using mAbs, may indicate a differ ence in recognized epitopes of NFs.…”

Section: Discussionmentioning

confidence: 99%

Expression of Subtypes NF-L, NF-M and NF-H of Neurofilament Triplet Proteins in the Developing Rat Inner Ear

Nishizaki

Anniko

1995

ORL

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The expression of each subtype (NF-L, NF-M and NF-H) of neurofilament (NF) triplet proteins in the developing rat inner ear was analysed using the immunofiuorescence method (FITC). On the 15th gestational day (GD), immunostaining of NF subtypes did not occur in the otic region. On the 18th GD, positivity for NF-L and NF-M occurred in nerve fibres in both the sensory and the subepithelial layers of the inner ear but was lacking for NF-H. At 4 days after birth immunoreactivity was present for each NF subtype but with topographic differences in their distribution. The cell bodies of (different?) subpopulations of vestibular and spiral ganglion cells were immunostained with monoclonal antibodies for NF-L and NF-M, but a subpopulation of both vestibular and spiral ganglion cells showed immunoreactivity for all three NF subtypes. The vestibular nerve fibres and ganglion cells evidenced stronger positivity than cochlear nerve fibres and spiral ganglion cells. This study confirms that the cytoskeletal maturation of inner ear nerve fibres precedes the onset of at least the auditory function by several days.

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“…However, unlike those in the spiral ganglion, none of the cells labeled as cNSCs in the organ of Corti also labeled for neuronal proteins such as NF 200 or NF M (Table 3). It should be noted that hair cells label with NF M during development but down regulate this marker by postnatal day 21 (Hasko et al, 1990). In our study, neither endogenous hair cells nor transplanted cells that localized to the conditions was compared to total RNA isolated from the OC2 cell line (OC; cochlear cell type positive control), a commercially available human fibroblast cell line (F; negative control), and whole brain (B; positive control).…”

Section: Cochlear Microenvironment Influences Cnsc Differentiationmentioning

confidence: 99%

Neural stem cells injected into the sound-damaged cochlea migrate throughout the cochlea and express markers of hair cells, supporting cells, and spiral ganglion cells

Parker

Corliss²,

Gray³

et al. 2007

Hearing Research

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Most cases of hearing loss are caused by the death or dysfunction of one of the many cochlear cell types. We examined whether cells from a neural stem cell line could replace cochlear cell types lost after exposure to intense noise. For this purpose, we transplanted a clonal stem cell line into the scala tympani of sound damaged mice and guinea pigs. Utilizing morphological, protein expression and genetic criteria, stem cells were found with characteristics of both neural tissues (satellite, spiral ganglion, and Schwann cells) and cells of the organ of Corti (hair cells, supporting cells). Additionally, noise-exposed, stem cell-injected animals exhibited a small but significant increase in the number of satellite cells and Type I spiral ganglion neurons compared to non-injected noise-exposed animals. These results indicate that cells of this neural stem cell line migrate from the scala tympani to Rosenthal's canal and the organ of Corti. Moreover, they suggest that cells of this neural stem cell line may derive some information needed from the microenvironment of the cochlea to differentiate into replacement cells in the cochlea.

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Transient expression of neurofilament protein during hair cell development in the mouse cochlea

Cited by 20 publications

References 14 publications

Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti

Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti

Expression of Subtypes NF-L, NF-M and NF-H of Neurofilament Triplet Proteins in the Developing Rat Inner Ear

Neural stem cells injected into the sound-damaged cochlea migrate throughout the cochlea and express markers of hair cells, supporting cells, and spiral ganglion cells

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