Transient increases in colony counts observed in declining populations of<i>Campylobacter jejuni</i>held at low temperature

Ekweozor, Chinyelu C; Nwoguh, C. E.; Barer, Michael R.

doi:10.1111/j.1574-6968.1998.tb12831.x

Cited by 11 publications

(1 citation statement)

References 21 publications

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“…While noting that these issues cannot be addressed ' by any finite series of experiments ' (Bogosian et al, 1998) we conclude that : (1) we have established a rigorous approach to the recognition of in vivo resuscitation events involving defined populations of nonculturable bacterial pathogens ; (2) ABNC cells meeting the criteria that others have used to recognize ' viable but nonculturable ' bacteria are not infective in our system ; (3) the presence of ABNC cells detected by the Kogure method cannot be considered predictive for the infective hazard posed by the material sampled ; and (4) at present, there are no validated means other than culture by which molecular signals obtained from clinically relevant samples can be shown to indicate the presence of infective bacteria. We urge others concerned with the issues addressed here to consider adopting comparable approaches to assessing the infectivity of nonculturable bacteria and hope that this will lead to a more precise view of whether and to what extent temporarily nonculturable bacteria (Barer & Harwood, 1999 ;Ekweozor et al, 1998) constitute a source and a reservoir for infective diseases.…”

Section: Discussionmentioning

confidence: 99%

Active but nonculturable cells of Salmonella enterica serovar Typhimurium do not infect or colonize mice

et al. 2002

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The possibility that nonculturable cells of a normally culturable bacterial pathogen may constitute a source or reservoir for infective disease was investigated. In multiple experiments and with careful attention to the statistical limitations of the assays used, Salmonella enterica serovar Typhimurium cells rendered nonculturable by carbon and nitrogen stress in the presence of chloramphenicol were administered orally and intraperitoneally to over 300 female BALB/c mice. Neither infection nor colonization was detected in these studies, even when active but nonculturable (ABNC) cells, as defined by the Kogure cell elongation assay, were present in the inoculum. Doses of ABNC cells exceeding the oral and intraperitoneal LD 50 values by 35 and 2 orders of magnitude, respectively, were administered. It was concluded that ABNC cells of the salmonella strains used could not be considered potentially infective and that their detection in samples from material being evaluated as a potential source or reservoir of infection by the Kogure test does not specifically represent an infective hazard.

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Section: Discussionmentioning

confidence: 99%

Active but nonculturable cells of Salmonella enterica serovar Typhimurium do not infect or colonize mice

et al. 2002

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The Public Health Significance of Viable but Nonculturable Bacteria

Oliver

2000

Nonculturable Microorganisms in the Environment

143

125

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Starvation Survival and Viable but Nonculturable States in Aeromonas hydrophila

Mary¹,

Chihib²,

Charafeddine

et al. 2002

Microbial Ecology

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The behavior of Aeromonas hydrophila stored at 4 degrees C and 25 degrees C in nutrient-poor filtered sterilized distilled water was investigated. At 4 degrees C, the A. hydrophila population declined below the detection level (0.1 cell mL(-1)) after 7 weeks, whereas the number of cells with intact membrane as determined by the LIVE/DEAD method decreased only by 1 log unit. Although, this response is reminiscent of the so-called VBNC state, the cells could not be resuscitated by an upshift to 25 degrees C. A mixture of rods with normal size and elongated cells was observed in this state. At 25 degrees C, viable cells and cells with intact membrane declined only by 0.8 log unit over the 10-week storage period, and thus A. hydrophila entered the classical starvation survival state. During this state, a mixture of rods and cocci was observed. Prestarvation at 25 degrees C for 24 h and especially 49 days delayed significantly the rate of entry into the VBNC state. However, stationary phase cells were not significantly more tolerant than exponential phase cells. No significant improvements in recovery yield were obtained on LB agar plates amended with catalase or sodium pyruvate. During cold incubation, high variability in responses was observed. Intermittent cryptic regrowth might be responsible for this variability in responses.

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Transient increases in colony counts observed in declining populations ofCampylobacter jejuniheld at low temperature

Cited by 11 publications

References 21 publications

Active but nonculturable cells of Salmonella enterica serovar Typhimurium do not infect or colonize mice

Active but nonculturable cells of Salmonella enterica serovar Typhimurium do not infect or colonize mice

The Public Health Significance of Viable but Nonculturable Bacteria

Starvation Survival and Viable but Nonculturable States in Aeromonas hydrophila

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