Translational control of protein synthesis and the control of steroidogenesis in the rabbit ovary

Gorski, Jack; Padnos, Donna

doi:10.1016/0003-9861(66)90161-5

Cited by 37 publications

(9 citation statements)

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“…In vitro studies with bovine corpus luteum slices (Savard et al 1965), rat ovarian cells (Kawano et al 1975) and isolated Graafian follicles (Lieberman et al 1975), actinomycin D has been demonstated to suppress the LH/hCG stimulated steroidogenesis. In contrast, Gorski & Padnos (1966) observed no effect of actinomycin D on gonadotrophin stimulated steroi¬ dogenesis in rabbit ovary. Further, studies by Marsh (1976) suggest that the inhibitory effect of actinomycin D on LH stimulated steroidogenesis in bovine corpus luteum is not observed consistently.…”

mentioning

confidence: 54%

“…Earlier work in this (Kawano et al 1975) and other laboratories (Marsh 1968;Savard et al 1965;Hermieretal. 1971;Lieberman et al 1975;Gorski & Padnos 1966) has suggested the possible involve¬ ment of a labile polypeptide factor(s) in gonado¬ trophin stimulated steroidogenesis. However, these studies were carried out with only one or two inhibitors which might give misleading results.…”

Section: Discussionmentioning

confidence: 99%

“…The intracellular mechanism bv which LH stimulates steroidogenesis and the role of macromolecules in this process are not fully understood. Although a requirement for macromolecular synthesis, particularly protein svnthesis, has often been proposed (Marsh 1968(Marsh , 1976Savard et al 1965;Hermier et al 1971;Kawano et al 1975;Lieberman et al 1975;Gorski & Padnos 1966), the exact nature of this requirement is still unknown. In most of these studies only a single inhibitor of protein synthesis, predominantly puromvcine or cycloheximide, was utilized to block the stimulatory effect of LH on steroidogenesis.…”

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confidence: 98%

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Receptor mediated gonadotropin action in the ovary

Azhar

Menon

1980

Acta Endocrinologica

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The role of macromolecular synthesis in gonadotrophin, cholera enterotoxin and cAMP stimulated progesterone production by rat ovarian cells has been investigated. Both progesterone and cAMP accumulation were increased by cholera enterotoxin in a concentration dependent manner. Incubation of cells with cholera enterotoxin resulted in a 10\p=n-\20-fold increase in cAMP levels. The effect was observed 15\p=n-\20 min after addition of toxin. In contrast, stimulation of cAMP levels by hCG was immediate. Cyclic AMP production in response to trophic hormone reached a maximum at 60 min, while maximum stimulation in response to toxin was attained after 2\ p=n-\ 3 h of incubation. Activation of steroidogenesis in response to both cholera enterotoxin and gonadotrophin showed a lag period. hCG and cholera enterotoxin stimulated a maximum amount of progesterone production after 2 and 3 h of incubation, respectively. With the maximum effective concentration of hCG, addition of choleragen did not result in any further increase in steroidogenesis. Similarly 8 Br-cAMP and Bt2-cAMP also stimulated steroidogenesis. Progesterone response to cholera enterotoxin, hCG, LH, 8 Br-cAMP and Bt2-cAMP was completely blocked by incubation of cells with cordycepin, actinomycin D, emetine and cycloheximide. That the specific effect of these inhibitors was on macromolecular synthesis rather than a general non-specific toxic effect was demonstrated by inhibition of [3H]proline incorporation and the lack of effect of these inhibitors on cAMP production in response to hCG, LH and cholera enterotoxin. Emetine (10 \g=m\m)and cycloheximide (50 \g=m\m) inhibited cholera enterotoxin and hCG stimulated progesterone production when added at different time points during the incubation. Cordycepin (250 \g=m\m) also blocked toxin and hCG induced steroidogenesis in a similar manner. The concentrations of cordycepin, cycloheximide and emetine required to completely block hCG stimulated progesterone production were 250, 50, and 5 \g=m\m, respectively. Similar concentrations of these inhibitors also maximally inhibited 8 Br-cAMP and cholera enterotoxin stimulated steroidogenesis. No effect of these inhibitors on basal production of progesterone was observed. These results suggest that gonadotrophin induced progesterone synthesis is dependent upon the continued synthesis of short lived mRNA and protein(s).The available evidence suggests that gonadotro¬ phin (LH/hCG) interaction with the ovarian system leads to activation of adenylate cyclase with resul¬ tant accumulation of cAMP and stimulation of

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confidence: 54%

Section: Discussionmentioning

confidence: 99%

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confidence: 98%

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Receptor mediated gonadotropin action in the ovary

Azhar

Menon

1980

Acta Endocrinologica

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“…23 The suggestion has been made that LH exerts its regulatory cont r o l at the translational level, but experiments in separate laboratories employing actinomycin D have led to opposing conclusions. 23 Means and Hall24 have recently reported that follicle-stimulating hormone (FSH) stimulates the biosynthesis of protein in the testes of rats aged 20 days but not in testes of O'Malley: Hormonal Regulation 481 older animals; information on the mechanism of this effect has not yet been reported.…”

Section: Peptide Hormonesmentioning

confidence: 97%

Division of Biochemistry: Hormonal Regulation of Nucleic Acid and Protein Synthesis*

O’Malley

1969

Trans NY Acad Sci

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“…V (3)). Gorski & Padnos (1966) suggest that luteinizing hormone can control translation, whilst Yatvin (1966a, b) considers that pituitary function is required to convert the inactive polysomes of chick feather cells to the active conformation (cf. VI).…”

Section: Hormonal E#ects On Protein Synthesismentioning

confidence: 99%

Addendum

1966

Biological Reviews

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Translational control of protein synthesis and the control of steroidogenesis in the rabbit ovary

Cited by 37 publications

References 9 publications

Receptor mediated gonadotropin action in the ovary

Receptor mediated gonadotropin action in the ovary

Division of Biochemistry: Hormonal Regulation of Nucleic Acid and Protein Synthesis*

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