Transmissible Burkholderia cepacia genomovar IIIa strains bind and convert monomeric iron(III) protoporphyrin IX into the μ-oxo oligomeric form

Screening and identification of a highly lipolytic bacterial strain from barbecue sites in Hainan and characterization of its lipase

“…Although BCC species are capable of causing life-threatening respiratory tract infections in persons with cystic fibrosis (Coenye et al 2001;Mahenthiralingam et al 2001;Magalhaes et al 2002), studies from several countries reiterate that two species, B. cenocepacia and B. multivorans, account for most BCC infections in cystic fibrosis (Smalley et al 2003;Lipuma 2005), while B. dolosa and B. vietnamiensis were occasionally isolated in cystic fibrosis (Coenye et al 2001;Lipuma 2005). The BCC species are also of ecological importance and some species have been isolated from diverse environments including soil, water, and plants (Singh et al 2006;Bartholdson et al 2008;Jacobs et al 2008).…”

Characterization of a bifunctional catalase–peroxidase ofBurkholderia cenocepacia

“…Smalley et al, unpublished results). Collectively, these data show that this component is not a true haem-binding protein, but a bifunctional catalase-peroxidase, in contrast to the 77-kDa and 149-kDa iron(III) protoporphyrin IX-binding, outer-membrane components, which do not possess intrinsic catalase activity, and which are only peroxidase-positive after exposure to, and binding of, iron(III) protoporphyrin IX monomers (Smalley et al, 2003). Multiple amino acid alignment analysis of the translated B. cenocepacia catalase-peroxidase gene revealed a strong homology with other bacterial catalase-peroxidases, and supported the above biochemical observations.…”

“…Smalley et al , unpublished results). Collectively, these data show that this component is not a true haem‐binding protein, but a bifunctional catalase–peroxidase, in contrast to the 77‐kDa and 149‐kDa iron(III) protoporphyrin IX‐binding, outer‐membrane components, which do not possess intrinsic catalase activity, and which are only peroxidase‐positive after exposure to, and binding of, iron(III) protoporphyrin IX monomers (Smalley et al , 2003).…”

“…It also has catalase activity, but is not inhibited by the specific monofunctional catalase inhibitor 3‐amino‐1,2,4,‐triazole. This protein does not show dose‐dependent binding of iron(III) protoporphyrin IX in either the monomeric or μ‐oxo oligomeric form (Smalley et al , 2003), and does not bind to haem–agarose (J.W. Smalley et al , unpublished results).…”

“…Burkholderia cenocepacia (genomovar IIIa) isolates are associated with life‐threatening lung infections that may progress to septicaemia (‘cepacia syndrome’) in cystic fibrosis (CF) patients (Hart & Winstanley, 2002). These bacteria were shown to express a 97‐kDa putative haem‐binding protein (Smalley et al , 2001), but more recent studies (Smalley et al , 2003) have shown that this protein does not bind haem dose‐dependently, and is peroxidase‐positive even without prior exposure to exogenous iron(III) protoporphyrin IX. These newer findings suggest the possibility that it is not a true haem‐binding protein.…”