Transplantations of frozen-thawed ovarian tissue demonstrate high reproductive performance and the need to revise restrictive criteria

Meirow, Dror; Raanani, Hila; Shapira, Moran; Brenghausen, Masha; Chaim, Sanaz Derech; Aviel‐Ronen, Sarit; Amariglio, Ninette; Schiff, Eyal; Orvieto, Raoul; Dor, Jehoshua

doi:10.1016/j.fertnstert.2016.04.031

Cited by 209 publications

(196 citation statements)

References 36 publications

(47 reference statements)

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“…This is on all the women Meirow and co-workers have described, both from the publication mentioned in Donnez and Dolmans' table [7] and Meirow and co-workers' last publication [14]. It is not possible to subdivide the 20 women according to [7] and the new cases mentioned in [15].…”

Section: Resultsmentioning

confidence: 99%

“…An overview of the perinatal outcome of children conceived in women who have had frozen-thawed ovarian tissue transplanted available from peer-reviewed papers, including nine new Danish cases, is given in Tables 2 [13,, 3, and 4. Table 2 provides detailed information on the deliveries from published peer-reviewed journals including information on the woman (diagnosis, age at cryopreservation and signs of menopause before transplantation, time from transplantation Oktay et al [13] S F N A 1 Meirow et al [14] SF NA* 4 (+2) The Nordic countries [15] and this publication SF (Sweden VF) 14 7(+2)** FertiPROTEKT network [16] SF 54*** 11 Dunlop et al [17] S F N A 1…”

Section: Resultsmentioning

confidence: 99%

“…Adapted from Donnez and Dolmans [7] SF slow freezing, VF vitrification a Twins b Personal communication *Meirow et al [14]: in total, 20 women have had transplantations performed and given birth to 10 children and 2 ongoing pregnancies. This is on all the women Meirow and co-workers have described, both from the publication mentioned in Donnez and Dolmans' table [7] and Meirow and co-workers' last publication [14].…”

Section: Resultsmentioning

confidence: 99%

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86 successful births and 9 ongoing pregnancies worldwide in women transplanted with frozen-thawed ovarian tissue: focus on birth and perinatal outcome in 40 of these children

Jensen

Macklon²,

Fedder

et al. 2016

J Assist Reprod Genet

248

160

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Purpose This study aims to make an account of the children born following transplantation of frozen-thawed ovarian tissue worldwide with specific focus on the perinatal outcome of the children. Furthermore, perinatal outcome of seven deliveries (nine children) from Denmark is reported. Methods PubMed was searched for papers of deliveries resulting from ovarian tissue cryopreservation (OTC). Seven women underwent OTC prior to chemotherapy. Four of these women still had low ovarian function and had tried to conceive. They therefore had tissue autotransplanted to augment their fertility. The other three women had developed premature ovarian insufficiency (POI) after the end of treatment.Results Worldwide, approximately 95 children have been born or will be born in the near future from OTC, including these 9 new children. Information on the perinatal outcome was found on 40 children. The mean gestational age was 39 weeks and the mean birth weight was 3168 g of the singleton pregnancies, which is within internationally recognized normal standards. Furthermore, half the singletons resulted from natural conception and all twins resulted from in vitro fertilization treatment. All seven Danish women became pregnant within 1-3 years after transplantation. They gave birth to nine healthy children. Conclusion The data is reassuring and further suggests that cryopreservation of ovarian tissue is becoming an established fertility preservation method. The seven Danish women reported in this study were all in their early thirties when OTC was performed. Most other reported cases were in the women's twenties. This suggests that the follicular pool in the thirties is large enough and sufficient to sustain fertility.Keywords Fertility preservation . Cryopreservation . Ovarian tissue transplantation . Deliveries IntroductionOvarian tissue cryopreservation (OTC) and transplantation is a relatively new procedure within the area of assisted reproduction technologies (ART). OTC differs from other ART procedures like freezing oocytes or embryos by cryopreserving intact tissue containing small non-growing follicles [1,2]. Although the cryoprotectant media used to freeze ovarian tissue resemble those media used for oocytes and embryos, there are often subtle differences [3,4]. Furthermore, extensive investigations on the effects of cryoprotectants on both human and animal ovarian tissue have not revealed any potential side effects. However, studies in women who had frozen-thawed ovarian tissue transplanted have The original version of this article was revised: There were errors in the Discussion section and the last paragraph should be removed.Capsule The data is reassuring and further suggests that cryopreservation of ovarian tissue is becoming an established fertility preservation method.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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86 successful births and 9 ongoing pregnancies worldwide in women transplanted with frozen-thawed ovarian tissue: focus on birth and perinatal outcome in 40 of these children

Jensen

Macklon²,

Fedder

et al. 2016

J Assist Reprod Genet

248

160

View full text Add to dashboard Cite

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“…Ovarian tissue reuse is still considered experimental, while some authors suggest that autotransplantation of frozen-thawed ovarian tissue should now be considered as established procedure for female fertility preservation [11–13]. Indeed, transplantation of frozen-thawed ovarian tissue has already resulted in 86 reported live births worldwide [14, 15] and, in a recent study including 111 women, the proportion of women who conceived after autograft of cryopreserved ovarian tissue was 29% [11].…”

Section: Introductionmentioning

confidence: 99%

Validation of an automated technique for ovarian cortex dissociation: isolation of viable ovarian cells and their qualification by multicolor flow cytometry

et al. 2017

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BackgroundOvarian tissue cryopreservation is a technique for fertility preservation addressed to prepubertal girls or to patients for whom no ovarian stimulation is possible before initiation of gonadotoxic treatments. Autotransplantation of frozen-thawed ovarian tissue is the only available option for reuse but presents some limitations: ischemic tissue damages post-transplant and reintroduction of malignant cells in cases of cancer. It is therefore essential to qualify ovarian tissue before autograft on a functional and oncological point of view. Here, we aimed to isolate viable cells from human ovarian cortex in order to obtain an ovarian cell suspension analyzable by multicolor flow cytometry.MethodsOvarian tissue (fresh or frozen-thawed), from patients with polycystic ovarian syndrome (reference tissue) and from patients who underwent ovarian tissue cryopreservation, was used for dissociation with an automated device. Ovarian tissue-dissociated cells were analyzed by multicolor flow cytometry; the cell dissociation yield and viability were assessed. Two automated dissociation protocols (named laboratory and commercial protocols) were compared.ResultsThe effectiveness of the dissociation was not significantly different between reference ovarian tissue (1.58 × 106 ± 0.94 × 106 viable ovarian cells per 100 mg of ovarian cortex, n = 60) and tissue from ovarian tissue cryopreservation (1.70 × 106 ± 1.35 × 106 viable ovarian cells, n = 18). However, the viability was slightly different for fresh ovarian cortex compared to frozen-thawed ovarian cortex whether we used reference tissue (p = 0.022) or tissue from ovarian cryopreservation (p = 0.018). Comparing laboratory and commercial protocols, it appeared that cell yield was similar but cell viability was significantly improved when using the commercial protocol (81.3% ± 12.3% vs 23.9% ± 12.5%).ConclusionBoth dissociation protocols allow us to isolate more than one million viable cells per 100 mg of ovarian cortex, but the viability is higher when using the commercial dissociation kit. Ovarian cortex dissociation is a promising tool for human ovarian cell qualification and for ovarian residual disease detection by multicolor flow cytometry.

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“…This method has resulted in more than 85 livebirths worldwide [2–4], with delivery rate of 25–32% per transplanted woman [2, 3]. …”

Section: Introductionmentioning

confidence: 99%

Spontaneous twin pregnancy with live births after cryopreservation and re-implantation of ovarian tissue

et al. 2017

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Transplantations of frozen-thawed ovarian tissue demonstrate high reproductive performance and the need to revise restrictive criteria

Abstract: NCT02659592.

Cited by 209 publications

References 36 publications

86 successful births and 9 ongoing pregnancies worldwide in women transplanted with frozen-thawed ovarian tissue: focus on birth and perinatal outcome in 40 of these children

86 successful births and 9 ongoing pregnancies worldwide in women transplanted with frozen-thawed ovarian tissue: focus on birth and perinatal outcome in 40 of these children

Validation of an automated technique for ovarian cortex dissociation: isolation of viable ovarian cells and their qualification by multicolor flow cytometry

Spontaneous twin pregnancy with live births after cryopreservation and re-implantation of ovarian tissue

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