Transport of cis- and trans-4-[18F]fluoro-L-proline in F98 glioma cells

Synthesis and Characterization of 18F-Interleukin-8 Using a Cell-Free Translation System and 4-18F-Fluoro-l-Proline

“…Therefore, we used human IL-8 RA-expressing xenograft bearing-mice to determine whether 18 F-IL-8 produced by the cell-free protein synthesis system works in vivo. 18 F-IL-8 rapidly accumulated in IL-8 RA-expressing xenografts and exhibited a good signal-to-background ratio in vivo; in contrast, trans-18 F-FPro exhibited low contrast as reported previously (32)(33)(34). Although full validation was required to characterize 18 F-IL-8 by doing inhibition/blocking study for the development of 18 F-IL-8 as a PET tracer, 18 F-IL-8 produced by this technique was biologically active in vitro and in vivo.…”

Transport of Pharmacologically Active Proline Derivatives by the Human Proton-Coupled Amino Acid Transporter hPAT1

“…Second, there are candidates for proline transport systems at the basolateral membrane, mainly system A, subtype ATA2. What has been reported for proline derivatives is that in F98 rat glioma cells cis-4-[ 18 F]fluoro-L-proline used for positron emission tomography scans is transported by system A in a Na ϩ -dependent manner (Langen et al, 2002). Fibroblast cell lines most sensitive to CHLP are those in which the activity of the A system is specifically increased (Ciardiello et al, 1988).…”

S-11C-Methyl-L-Cysteine: A New Amino Acid PET Tracer for Cancer Imaging

“…The method of measuring transport was similar to a method reported previously (2,16,17). The competitive inhibition experiments to characterize the transport system were performed in the presence of the specific competitive inhibitors: for system L, 29-aminobicyclo(2,2,1)-heptane-29-carboxylic acid (BCH); for system A, N-methylamino-isobutyric acid (MeAIB); and for system ASC, MeAIB plus serine.…”