Transport of lysolecithin by albumin in human and rat plasma

Interaction of spin-labeled lysophosphatidylcholine with rabbit erythrocytes

“…The rates of hemolysis and line broadening showed similar temperature dependences. Thus the interaction of the lyso-3 Bovine serum albumin is well known to combine with egg yolk lysophosphatidylcholine (Switzer & Eder, 1965;Klopfestein, 1969). In fact, spin-labeled lysophosphatidylcholine interacted with serum albumin; however, this interaction immobilized the spin-labeled lysophosphatidylcholine and resulted in a completely different ESR spectrum from that shown in Figure 3.…”

Interactions of Lyso 1-Palmitoylphosphatidylcholine with Phospholipids: A 13C and 31P NMR Study

“…Albumin binds lysoPC and may serve to transport lysoPC in plasma (Switzer & Eder, 1965). Bovine serum albumin (BSA) has been used to characterize lysoPC distribution and transbilayer movement in erythrocyte membranes (Bergmann et al, 1984a,b) and to extract lysoPC from erythrocyte membranes hydrolyzed by PLA2 to understand bilayer stability and cell shape changes following lipid removal mole ratios of FA to LPPC: 1:1 (O), 1:2 ( ), 1:5 (V), and 2:1 (0).…”

Studies of the Composition and Structure of Serum Lipoproteins. Isolation and Characterization of Very High Density Lipoproteins of Human Serum*

“…The latter VHDL preparation contained 96% protein and 4% phospholipid. Switzer and Eder (1965) found, on the basis of electrophoresis, salt precipitation, and gel filtration experiments, that the major portion of plasma lysolecithin is bound to albumin. This finding is of particular interest since it may provide the explanation for the striking difference in the phospholipid composition of VHDLi and VHDL,.…”

“…Lipid and Protein Analyses. Esterified and unesterified cholesterol was determined according to the method of Sperry and Webb (1950), lipid phosphorus by the method of Fiske and Subbarow (1925), the triglycerides by the procedure described by Van Handel and Zilversmit (1957), and the unesterified fatty acids by the method of Dole (1952). Quantitative analysis of individual phospholipids was performed by twodimensional thin layer chromatography by a modification (Gustafson et al, 1966) of the method described by Wagner et al (1961).…”

“…of very high density human serum lipoproteins (d > 1.21 g/ml). port of serum lysolecithin, Switzer and Eder (1965) concluded that as much as 96% of the total serum lysolecithin is bound to albumin in the d > 1.21 fraction and suggested that the negligible amount of phospholipid, other than lysolecithin, is carried by a ultracentrifugally altered HDL. However, since VHDL have not been isolated as a single entity, the controversy regarding their origin, chemical composition, and the nature of their protein moiety or moieties has not been resolved.…”