Trapping and releasing of single microparticles and cells in a microfluidic chip

Lv, Dan; Zhang, Xiaoling; Xu, Mengli; Cao, Wenyue; Liu, Xing; Deng, Jiajia; Yang, Jun; Hu, Ning

doi:10.1002/elps.202200091

Cited by 9 publications

(5 citation statements)

References 34 publications

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“…Moreover, particle recycling from the microfluidic system should also be considered to further improve the system for other downstream studies. One possible solution is to add microelectrodes that generate negative dielectrophoresis to selectively release target particles 37 . Overall, the device is beneficial for accurate microplastic detection, primarily due to its capability to trap an extremely small number of particles in a single trap.…”

Section: Resultsmentioning

confidence: 99%

A microfluidic approach for label-free identification of small-sized microplastics in seawater

Gong

Martínez

Mesquita

et al. 2023

Sci Rep

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Marine microplastics are emerging as a growing environmental concern due to their potential harm to marine biota. The substantial variations in their physical and chemical properties pose a significant challenge when it comes to sampling and characterizing small-sized microplastics. In this study, we introduce a novel microfluidic approach that simplifies the trapping and identification process of microplastics in surface seawater, eliminating the need for labeling. We examine various models, including support vector machine, random forest, convolutional neural network (CNN), and residual neural network (ResNet34), to assess their performance in identifying 11 common plastics. Our findings reveal that the CNN method outperforms the other models, achieving an impressive accuracy of 93% and a mean area under the curve of 98 ± 0.02%. Furthermore, we demonstrate that miniaturized devices can effectively trap and identify microplastics smaller than 50 µm. Overall, this proposed approach facilitates efficient sampling and identification of small-sized microplastics, potentially contributing to crucial long-term monitoring and treatment efforts.

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Section: Resultsmentioning

confidence: 99%

A microfluidic approach for label-free identification of small-sized microplastics in seawater

Gong

Martínez

Mesquita

et al. 2023

Sci Rep

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“…The design of the main channel containing cup-shaped microtraps is based on hydrodynamic principles ,, (Figure A). When a GUV-containing suspension was injected into the channel, a GUV was quickly captured by the first microtrap due to the lower flow resistance within the trap than that in the main channel.…”

Section: Resultsmentioning

confidence: 99%

Microarray Chip and Method for Simultaneous and Highly Consistent Electroporation of Multiple Cells of Different Sizes

Mou

Bai

et al. 2023

Anal. Chem.

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Cell electroporation is an important cell manipulation technology to artificially transfer specific extracellular components into cells. However, the consistency of substance transport during the electroporation process is still an issue due to the wide size distribution of the natural cells. In this study, a cell electroporation microfluidic chip based on a microtrap array is proposed. The microtrap structure was optimized for single-cell capture and electric field focusing. The effects of the cell size on the cell electroporation in the microchip were investigated through simulation and experiment methods using the giant unilamellar vesicle as the simplified cell model, and a numerical model of a uniform electric field was used as a comparison. Compared with the uniform electric field, a lower threshold electric field is required to induce electroporation and produces a higher transmembrane voltage on the cell under a specific electric field in the microchip, showing an improvement in cell viability and electroporation efficiency. The larger perforated area produced on the cells in the microchip under a specific electric field allows a higher substance transfer efficiency, and the electroporation results are less affected by the cell size, which is beneficial for improving substance transfer consistency. Furthermore, the relative perforation area increases with the decrease of the cell diameter in the microchip, which is exactly opposite to that in a uniform electric field. By manipulating the electric field applied to the microtrap individually, a consistent proportion of substance transfer during electroporation of cells with different sizes can be achieved.

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“…Microdroplets are guided into the trapping pockets by making the flow resistance of the pocket and side channel ( R side ) smaller than that of the main channel ( R main ). When a droplet is trapped in a pocket, the flow resistance increases and subsequent droplets pass through the main channel to the next pocket without being trapped in the same pocket section [ 12 , 13 , 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…In addition, many studies require selective extraction of the cells from the device after screening for subsequent off-chip analysis of more specific responses. Recently, Lv et al [ 12 ] and Zhu et al [ 13 ] successfully trapped cells hydrodynamically and selectively extracted them from the device by DEP, while Kim et al [ 14 ] were able to trap cells using microvalves and selectively extract them to specific locations by applying backflow. However, conventional microfluidic devices capture bare cells directly in the channel, which can cause cell damage due to the pressure used for capture or contamination in the fluid.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoresis-Based Selective Droplet Extraction Microfluidic Device for Single-Cell Analysis

et al. 2023

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We developed a microfluidic device that enables selective droplet extraction from multiple droplet-trapping pockets based on dielectrophoresis. The device consists of a main microchannel, five droplet-trapping pockets with side channels, and drive electrode pairs appropriately located around the trapping pockets. Agarose droplets capable of encapsulating biological samples were successfully trapped in the trapping pockets due to the difference in flow resistance between the main and side channels. Target droplets were selectively extracted from the pockets by the dielectrophoretic force generated between the electrodes under an applied voltage of 500 V. During their extraction from the trapping pockets, the droplets and their contents were exposed to an electric field for 400–800 ms. To evaluate whether the applied voltage could potentially damage the biological samples, the growth rates of Escherichia coli cells in the droplets, with and without a voltage applied, were compared. No significant difference in the growth rate was observed. The developed device enables the screening of encapsulated single cells and the selective extraction of target droplets.

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Trapping and releasing of single microparticles and cells in a microfluidic chip

Cited by 9 publications

References 34 publications

A microfluidic approach for label-free identification of small-sized microplastics in seawater

A microfluidic approach for label-free identification of small-sized microplastics in seawater

Microarray Chip and Method for Simultaneous and Highly Consistent Electroporation of Multiple Cells of Different Sizes

Dielectrophoresis-Based Selective Droplet Extraction Microfluidic Device for Single-Cell Analysis

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