Treatment of dental biofilm with a tincture of Bauhinia forficata leaves: an ex-vivo study

Abstract

The inhibitory activity of a Bauhinia forficata tincture (TBF) was investigated against oral microorganism's strains and against a mature oral biofilm. The viability of planktonic cells was analyzed by Minimal Inhibitory and Microbicidal concentrations of TBF. Salivary samples from health volunteers were collected and mixed to form a saliva pool. An aliquot from this pool were seeded on membranes, which were incubated to form biofilm (48 h). The biofilm was treated according to the groups: G1-Chlorhexidine 0.12%; G2-TBF at the highest MMC; G3-Ethanol at the TBF highest MMC. G4 was the growth control. Streptococcus spp. (S) and total microorganisms (TM) from biofilm were counted. TBF was microbicidal against all oral pathogens. G2 was able to reduce the counts of S and TM from biofilm compared to G3 and G4, but less than G1 (p < 0.05). TBF is able to reduce the microbial levels from a mature oral biofilm.

Emerging strategies for combating Fusobacterium nucleatum in colorectal cancer treatment: Systematic review, improvements and future challenges

“…Bauhinia forficata tincture Phenol, flavonoids, acid derivatives [90] Berberis goudotii Tannin, polyphenol [76] Stem-leaf American ginseng Saponin Terpenes impede the peptidation of the expanding peptidoglycan chain and cell wall production enzymes. [100] [91]…”

“Cow’s Hoof” (Bauhinia L., Leguminosae): A Review on Pharmacological Properties of Austral South American Species

“…Thus, B. forficata extract could be potentially used—together with norfloxacin—to treat infections caused by multidrug-resistant S. aureus [ 73 ]. Ferreira-Filho et al [ 71 , 72 ] showed antimicrobial effects of B. forficata leaf tincture (20% in a 70% hydroethanolic solution) against oral microorganism strains and mature dental biofilms obtained from salivary samples and formed on membranes or bovine enamel blocks. The tincture is a promising preventive agent of dental caries, with no cytotoxic effect, tested against oral fibroblast cells.…”

“…Toxic effects were not reported in most methodological approaches (e.g., [ 72 , 76 , 84 , 89 , 97 , 138 , 141 , 173 ]), however some research findings deserve attention. It has been reported that acute treatment with B. forficata crude extract (2.85 g/kg) injected intraperitoneally caused the death of 50% of the animals, yet oral administration (0.5 to 5.0 g/kg) is not toxic [ 1 ].…”

Monografias de Plantas Medicinais Brasileiras e Aclimatadas: Volume II

“…(Adewole, Caxton Martins & Ojewole, 2007;Ferreres et al, 2012;Jorge et al, 2004;Kim et al, 2011;Meneses et al, 2007;Pinheiro et al, 2006;Salgueiro et al, 2013;Sousa et al, 2004;Tzeng et al, 2009;Yamasaki et al, 2011;Zanatta et al, 2008). (Ferreira Filho et al, 2018). Outros autores acharam a atividade antibacteriana em B. forficata e B. variegata fraca ou nula (Silva & Cechinel Filho, 2002;Martinez et al, 2011).…”