Tree Tissue Culture and Ex Vitro Sand Rooting for Reforestation

Saiju, H. K.

doi:10.1007/4-431-28054-5_14

Plantation Technology in Tropical Forest Science

DOI: 10.1007/4-431-28054-5_14

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Tree Tissue Culture and Ex Vitro Sand Rooting for Reforestation

H. K. Saiju¹

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Cited by 4 publications

(2 citation statements)

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“…For some species that are not easy to root and acclimatize, especially woody plant species, microshoots have been successfully rooted ex vitro and the plants then survived when planted in the field (Saiju 2005;Annapurna and Rathore 2010;Liu et al 2010;Huang et al 2011). The number of roots and the quality of the rooting system of such plants are assumed to be more suitable than those formed in vitro (McClelland et al 1990).…”

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confidence: 98%

Micropropagation and ex vitro rooting of pistachio (Pistacia vera L.)

Benmahioul

Dorion

Kaïd-Harche

et al. 2011

Plant Cell Tiss Organ Cult

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An effective pistachio (Pistacia vera L.) micropropagation system was developed involving rapid axillary bud proliferation and ex vitro rooting. The highest shoot proliferation frequency was obtained from nodal explants cultured on Murashige and Skoog (MS) basal salts containing Gamborg (B 5 ) vitamins and supplemented with 4 mg l -1 6-benzyladenine (BA). The addition of 2 mg l -1 meta-topolin (mT) generated an optimal number of shoots with suitable morphological features, while kinetin (KIN) was found to be unsuitable for pistachio shoot proliferation. Microcuttings were rooted ex vitro after being dipped in rooting powder. The peak ex vitro rooting response was achieved after shoot explants were treated with Rhizopon Ò 2% indole-3-butyric acid (IBA). Rooted plantlets were transplanted in plastic pots containing a peat-perlite-vermiculite (1:1:1) mixture and then transferred to the greenhouse. After 2 months, 81.5% survival of rooted microshoots was achieved.

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mentioning

confidence: 98%

Micropropagation and ex vitro rooting of pistachio (Pistacia vera L.)

Benmahioul

Dorion

Kaïd-Harche

et al. 2011

Plant Cell Tiss Organ Cult

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“…For many fruit species that are not easy to root and acclimatize, the rooting phase has been successfully performed ex vitro and the plants then survived when planted in the field (Benmahioul et al, 2012;Saiju, 2005). The present work has been conducted to develop an efficient and reliable protocol for the micropropagation of wolfberry (Lycium barbarum L.), focusing on the effects of mineral salts, growth regulators, and in vitro and ex vitro rooting.…”

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Micropropagation and Ex Vitro Rooting of Wolfberry

Silvestri¹,

Sabbatini²,

Marangelli³

et al. 2018

horts

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Additional index words. Lycium barbarum, axillary shoot proliferation, in vitro and ex vitro rooting, acclimatization, growth regulatorsAbstract. An accurate protocol for the in vitro propagation of a commercial wolfberry (Lycium barbarum L.) cv. Nixia 1 has been developed through axillary shoot proliferation. Driver and Kuniyuki Walnut (DKW) medium supplemented with 6-benzylaminopurine (BAP; 0.5 mg/L) and sucrose 3% w/v gave the best results compared with other basal media tested, with significantly improved production of multiple shoots direct from nodal segment explants, resulting in an average of 6.73 shoots/explant with an average of 7.45 nodes/shoot that would potentially form new explants. Rooting of shoot explants was carried out both in vitro and ex vitro with 0.5 and 1 mg/L of indole-3-butyric acid (IBA), with or without adding putrescine (160 mg/L). In all cases, rooting efficiency resulted very high, and putrescine was effective only when combined with a low concentration of auxin. Plantlets were hardened off in jiffy pots under greenhouse conditions, with a survival rate of more than 90%. Ex vitro rooting, performed by dipping in an aqueous solution of IBA 100 mg/L, is the preferred technique not only because rooting and acclimatization are very high but also reducing micropropagation to one phase is more economical.

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Micropropagation of Corema album from adult plants in semisolid medium and temporary immersion bioreactor

Alves

Pinto

Debiasi

et al. 2021

Plant Cell Tiss Organ Cult

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Tree Tissue Culture and Ex Vitro Sand Rooting for Reforestation

Cited by 4 publications

References 1 publication

Micropropagation and ex vitro rooting of pistachio (Pistacia vera L.)

Micropropagation and ex vitro rooting of pistachio (Pistacia vera L.)

Micropropagation and Ex Vitro Rooting of Wolfberry

Micropropagation of Corema album from adult plants in semisolid medium and temporary immersion bioreactor

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