TREE1-EIN3–mediated transcriptional repression inhibits shoot growth in response to ethylene

Abstract

Ethylene is an important plant hormone that regulates plant growth, in which the master transcriptionactivator EIN3 (Ethylene Insensitive 3)-mediated transcriptional activation plays vital roles. However, the EIN3-mediated transcriptional repression in ethylene response is unknown. We report here that a Transcriptional Repressor of EIN3-dependent Ethylene-response 1 (TREE1) interacts with EIN3 to regulate transcriptional repression that leads to an inhibition of shoot growth in response to ethylene. Tissue-specific transcriptome analysis showed that most of the genes are down-regulated by ethylene in shoots, and a DNA binding motif was identified that is important for this transcriptional repression. TREE1 binds to the DNA motif to repress gene expression in an EIN3-dependent manner. Genetic validation demonstrated that repression of TREE1-targeted genes leads to an inhibition of shoot growth. Overall, this work establishes a mechanism by which transcriptional repressor TREE1 interacts with EIN3 to inhibit shoot growth via transcriptional repression in response to ethylene.

Genome-Wide Identification and Expression Patterns of the C2H2-Zinc Finger Gene Family Related to Stress Responses and Catechins Accumulation in Camellia sinensis [L.] O. Kuntze

“…Among them, clade C had the most members, followed by clade A and clade B. Clade A, B and C consisted of 5, 51 and 71 CsC2H2-ZFPs, respectively. Clade A contained the known TREE1 (At4g35610) and DAZ3 (At4g35700) which interact with EIN3 to inhibit shoot growth in response to ethylene [ 34 ]. Clade B had seven CsC2H2-ZFPs, including the PCFS4 (AT4G04885), which regulates FCA mRNA alternative processing to promote flowering in Arabidopsis [ 35 ].…”

The coordination of melatonin and anti‐bacterial activity by EIL5 underlies ethylene‐induced disease resistance in cassava

“…Ethylene and the key components of the ethylene signaling pathway are widely implicated in plant development and environmental stress responses (Dubois et al., 2018; Pesquet & Tuominen, 2011; Wang et al., 2020a; Xiao et al., 2021). Melatonin is also essential for multiple physiological responses, especially plant immune responses (Arnao & Hernández‐Ruiz, 2019, 2021).…”

“…Phytohormones such as auxin, abscisic acid (ABA), gibberellic acid (GA), cytokinin (CK), brassinosteroids (BRs), salicylic acid (SA), jasmonic acid (JA), and ethylene play important roles in mediating defense signal transduction (Bari & Jones, 2009; Mersmann et al., 2010). Among them, ethylene is widely involved in plant development and environmental stress responses (Dubois et al., 2018; Ma et al., 2018; Pesquet & Tuominen, 2011; Shi et al., 2012; Wang et al., 2020a; Zhao et al., 2020b; Zhao et al., 2020a). During plant immune responses, ethylene is associated with pathogen‐associated molecular pattern (PAMP)‐triggered immunity (PTI) and systemic acquired resistance (SAR) (Mersmann et al., 2010; van Loon et al., 2006).…”

Ethylene Signaling Facilitates Plant Adaption to Physical Barriers

“…EIN2-C is also able to translocate from the cytoplasm to the nucleus, where it associates with a histone binding protein for epigenetically facilitating EIN3/EIL1-dependent transcriptional regulation ( Zhang et al, 2017 ). EIN3/EIL1 can function as a transcriptional activator or repressor in response to ethylene ( Wang et al, 2020 ).…”