Trichoderma harzianum strain T-22 induces changes in phytohormone levels in cherry rootstocks (Prunus cerasus × P. canescens)

Abstract

The aim of this research was to explain the direct plant growth-promoting activity of Trichoderma harzianum strain T-22 (T22), hypothesizing the involvement of different classes of plant growth regulators. Seven days after the transfer to root-inducing medium, in vitro-cultured shoots of GiSeLa6 Ò (Prunus cerasus 9 P. canescens) were inoculated with T22. Root and shoot growth were significantly affected by T22 (?76 and ?61%, respectively). Ten days after inoculation, the levels of indole-3-acetic acid (IAA), trans-zeatin riboside (t-ZR), dihydrozeatin riboside (DHZR), gibberellic acid (GA3) and abscisic acid (ABA) were analyzed by high performance liquid chromatography coupled with mass spectrometry. The results showed that after T22-inoculation, IAA and GA3 significantly increased in both leaves (?49 and ?71%, respectively) and roots (?40 and ?143%, respectively) whereas t-ZR decreased (-51% in leaves and -37% in roots). Changes in DHZR were observed in T22-inoculated roots (-32%) but not in leaves, whereas the levels of ABA did not differ between the two treatments. The extraction method allowed the simultaneous extraction of phytohormones. There is evidence that the change in phytohormone levels is one of the direct mechanism by which T22 promotes rooting and shoot growth, with notable advantages for rootstock production during nursery processes.

Alleviation of abiotic salt stress inOchradenus baccatus(Del.) byTrichoderma hamatum(Bonord.) Bainier

“…In many studies Trichoderma spp. could improve the growth of some medicinal plants by producing phytohormones such as gibberellins, auxin, and cytokinins (Sofo et al 2011;Hanefat et al 2012;Martínez-Medina et al 2014;Resende et al 2014).…”

Ethylene and auxin interaction in the control of adventitious rooting inArabidopsis thaliana

“…The lower phase was removed (750 μl), concentrated using an evaporator with nitrogen flow, and then re-dissolved in 15 μl methanol. Quantitative determinations of IAA and IBA were carried out by high-performance liquid chromatography coupled with mass spectrometry, according to Sofo et al (2011) (Supplementary Fig. S1 at JXB online).…”

ABA plays essential roles in regulating root growth by interacting with auxin and MAPK signaling pathways and cell-cycle machinery in rice seedlings

“…Subsequently, 1.0 mL of the solvent from the lower phase was concentrated using an evaporator with nitrogen flow and re-dissolved in 0.1 mL of cold methanol. The concentrations of IAA were determined by high-performance liquid chromatography coupled with mass spectrometry (HP1100 HPLC, Agilent Inc.; esquire 2000 MS Bruker Daltonics Inc., USA), according to Sofo et al (2011).…”