Triethylene Glycol Dimethacrylate Induction of Apoptotic Proteins in Pulp Fibroblasts

Batarseh, Ghada; Windsor, L. Jack; Labban, NY; Liu, Y; Gregson, Karen S.

doi:10.2341/12-417-l

Cited by 22 publications

(9 citation statements)

References 25 publications

(39 reference statements)

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“…It is to be noted that in the presence of a high dose of TEGDMA (3 mM) no results were obtained by flow cytometry, TRAP staining, qRT-PCR, or ELISA analyses. Consistent with previous studies indicating that TEGDMA can activate apoptotic pathways 3 , 15 , this study revealed that TEGDMA caused a dose-dependent increase in apoptosis of hDPCs and cell viability has been adversely affected in the presence of high doses of TEGDMA. Therefore, it has not been possible to carry out studies on the effects of high doses of TEGDMA on odontoclastic differentiation ability of hDPCs.…”

Section: Discussionsupporting

confidence: 92%

Effects of triethylene glycol dimethacrylate (TEGDMA) on the odontoclastic differentiation ability of human dental pulp cells

Torun¹,

Torun

Baykal

et al. 2017

J. Appl. Oral Sci.

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Objectives:The primary purpose of this study was to examine the effects of triethylene glycol dimethacrylate (TEGDMA) on odontoclastic differentiation in the dental pulp tissue.Material and Methods:The effects of different TEGDMA dosages on the odontoclastic differentiation capability of dental pulp cells were analyzed in vitro using the following methodologies: i) flow cytometry and tartrate-resistant acid phosphatase (TRAP) staining; ii) apoptotic effects using Annexin V staining; iii) mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor (NF)-kB ligand (RANKL) genes by quantitative Real-time PCR (qRT-PCR); and iv) OPG and RANKL protein expression by enzyme-linked immunosorbent assay (ELISA).Results:TEGDMA caused relatively less odontoclastic differentiation in comparison with the control group; however, odontoclastic differentiation augmented with increasing doses of TEGDMA (p<0.05). The mRNA and protein expression of OPG was lower in TEGDMA treated pulp cells than in the control group (p<0.05). While the mRNA expression of RANKL remained unchanged compared to the control group (p>0.05), its protein expression was higher than the control group (p<0.05). In addition, TEGDMA increased the apoptosis of dental pulp cells dose dependently.Conclusions:TEGDMA reduced the odontoclastic differentiation ability of human dental pulp cells. However, odontoclastic differentiation ratios increased proportionally with the increasing dose of TEGDMA.

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Section: Discussionsupporting

confidence: 92%

Effects of triethylene glycol dimethacrylate (TEGDMA) on the odontoclastic differentiation ability of human dental pulp cells

Torun¹,

Torun

Baykal

et al. 2017

J. Appl. Oral Sci.

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“…It is co-activated by TGF-β activated SMAD2 via a transactivation crosstalk with TCF4 ( 33 ). Upregulated β-catenin triggers transcriptional activation of SNAIL2-mediated EMT and proliferation signaling ( 31 , 35 ). TD-10 and TD-11 caused decrease in β-catenin implying inhibition of the canonical Wnt/β-catenin pathway yielding attenuation of the EMT as well as cell proliferation signaling.…”

Section: Discussionmentioning

confidence: 99%

“…Cytotoxicity of TD-10 has also been reported in some other studies. Batarseh et al ( 35 ) demonstrated induction of apoptotic signaling in human pulp fibroblasts by TD-10. By analyzing human apoptotic array, they found that TD-10 treated cells showed sustained upregulation of BH3 family proteins, caspase 3/8, and cytochrome c release.…”

Section: Discussionmentioning

confidence: 99%

Identification and Functional Characterization of Anti-metastasis and Anti-angiogenic Activities of Triethylene Glycol Derivatives

Garg

Liu

et al. 2018

Front. Oncol.

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We had previously reported anticancer activity in the water extract (WEX) of Ashwagandha leaves, and identified Triethylene glycol (TEG) as an active tumor suppressor component. In this study, we investigated anti-migratory and anti-angiogenesis activities of WEX and TEG. We conducted in vitro and in vivo experiments using TEG, and its two derivatives, Triethyleneglycol dimethacrylate (TD-10), and Tetraethyleneglycol dimethacrylate (TD-11). The data revealed strong anticancer and anti-metastasis potentials in the derivatives. Non-toxic, anti-migratory doses of the derivatives showed inhibition of canonical Wnt/β-catenin axis and consequent downregulation of EMT-signaling proteins (Vimentin, MMPs and VEGF). These results endorse that the TD-10 and TD-11 have potential to safely put a check on the aggressiveness of the metastatic cells and therefore represent promising candidates for the treatment of metastatic cancers.

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“…Declining ATP levels and compromised redox balance as a result of mitochondrial damage are common causes of regulated cell death . Triethylene glycol dimethacrylate has previously been demonstrated to cause apoptosis through the intrinsic and extrinsic pathways . In the GO enrichment analysis, extrinsic apoptotic signaling pathways were advocated for the group treated with 2.5 mM TEGDMA.…”

Section: Discussionmentioning

confidence: 99%

Dose‐ and time‐dependent effects of triethylene glycol dimethacrylate on the proteome of human THP‐1 monocytes

Nilsen

Simón-Santamaría

Örtengren

et al. 2018

European J Oral Sciences

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Triethylene glycol dimethacrylate (TEGDMA) is commonly used in polymer resin-based dental materials. This study investigated the molecular mechanisms of TEGDMA toxicity by identifying its time- and dose-dependent effects on the proteome of human THP-1 monocytes. The effects of different concentrations (0.07-5 mM) and exposure times (0-72 h) of TEGDMA on cell viability, proliferation, and morphology were determined using a real-time viability assay, automated cell counting, and electron microscopy, and laid the fundament for choice of exposure scenarios in the proteomic experiments. Solvents were not used, as TEGDMA is soluble in cell culture medium (determined by photon correlation spectroscopy). Cells were metabolically labeled [using the stable isotope labeled amino acids in cell culture (SILAC) strategy], and exposed to 0, 0.3 or 2.5 mM TEGDMA for 6 or 16 h before liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. Regulated proteins were analyzed in the STRING database. Cells exposed to 0.3 mM TEGDMA showed increased viability and time-dependent upregulation of proteins associated with stress/oxidative stress, autophagy, and cytoprotective functions. Cells exposed to 2.5 mM TEGDMA showed diminished viability and a protein expression profile associated with oxidative stress, DNA damage, mitochondrial dysfunction, and cell cycle inhibition. Altered expression of immune genes was observed in both groups. The study provides novel knowledge about TEGDMA toxicity at the proteomic level. Of note, even low doses of TEGDMA induced a substantial cellular response.

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Triethylene Glycol Dimethacrylate Induction of Apoptotic Proteins in Pulp Fibroblasts

Abstract: The results indicated that TEGDMA activates both the extrinsic and intrinsic apoptotic pathways.

Cited by 22 publications

References 25 publications

Effects of triethylene glycol dimethacrylate (TEGDMA) on the odontoclastic differentiation ability of human dental pulp cells

Effects of triethylene glycol dimethacrylate (TEGDMA) on the odontoclastic differentiation ability of human dental pulp cells

Identification and Functional Characterization of Anti-metastasis and Anti-angiogenic Activities of Triethylene Glycol Derivatives

Dose‐ and time‐dependent effects of triethylene glycol dimethacrylate on the proteome of human THP‐1 monocytes

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