Trinuclear copper biocatalytic center forms an active site of thiocyanate dehydrogenase

Tikhonova, Т. V.; Sorokin, Dimitry Y.; Hagen, Wilfred R.; Khrenova, Maria G.; Muyzer, Gerard; Rakitina, Tatiana V.; Shabalin, I.G.; Trofimov, A.A.; Tsallagov, S.I.; Popov, Vladimir O.

doi:10.1073/pnas.1922133117

Cited by 21 publications

(29 citation statements)

References 48 publications

(50 reference statements)

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“…Speculatively, it is also possible, where dissolved Cu concentrations were observed to increase slightly towards the end of our experiments, that release of Cu from SCN − -degrading bacterial cells, in stationary and/or death phases, could explain this observation. Previous findings of the incorporation of Cu in one of the main enzymes involved in autotrophic SCN − biodegradation (Tikhonova et al 2020), along with the inhibitory effect of cell-associated Cu (De Schamphelaere et al 2005), would support this speculation.…”

Section: Discussionmentioning

confidence: 84%

The effect of heavy metals on thiocyanate biodegradation by an autotrophic microbial consortium enriched from mine tailings

Shafiei

Watts

Pajank

et al. 2020

Appl Microbiol Biotechnol

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Bioremediation systems represent an environmentally sustainable approach to degrading industrially generated thiocyanate (SCN−), with low energy demand and operational costs and high efficiency and substrate specificity. However, heavy metals present in mine tailings effluent may hamper process efficiency by poisoning thiocyanate-degrading microbial consortia. Here, we experimentally tested the tolerance of an autotrophic SCN−-degrading bacterial consortium enriched from gold mine tailings for Zn, Cu, Ni, Cr, and As. All of the selected metals inhibited SCN− biodegradation to different extents, depending on concentration. At pH of 7.8 and 30 °C, complete inhibition of SCN− biodegradation by Zn, Cu, Ni, and Cr occurred at 20, 5, 10, and 6 mg L−1, respectively. Lower concentrations of these metals decreased the rate of SCN− biodegradation, with relatively long lag times. Interestingly, the microbial consortium tolerated As even at 500 mg L−1, although both the rate and extent of SCN− biodegradation were affected. Potentially, the observed As tolerance could be explained by the origin of our microbial consortium in tailings derived from As-enriched gold ore (arsenopyrite). This study highlights the importance of considering metal co-contamination in bioreactor design and operation for SCN− bioremediation at mine sites. Key points • Both the efficiency and rate of SCN−biodegradation were inhibited by heavy metals, to different degrees depending on type and concentration of metal. • The autotrophic microbial consortium was capable of tolerating high concentrations of As, potential having adapted to higher As levels derived from the tailings source.

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Section: Discussionmentioning

confidence: 84%

The effect of heavy metals on thiocyanate biodegradation by an autotrophic microbial consortium enriched from mine tailings

Shafiei

Watts

Pajank

et al. 2020

Appl Microbiol Biotechnol

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“…100 mg) in the human body [20]. It works as a cofactor that binds to various metalloenzymes and assists their activation [4,21]. Since it usually exists as cuprous ions [Cu(I)] and cupric ions [Cu(II)], Cu(I/II) can serve as an electron transporter.…”

Section: Cu(i/ii) 21 Cu(i/ii) Distributions In the Nervous Systemmentioning

confidence: 99%

Redox-Active Metal Ions and Amyloid-Degrading Enzymes in Alzheimer’s Disease

Kim

Lee

2021

IJMS

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Redox-active metal ions, Cu(I/II) and Fe(II/III), are essential biological molecules for the normal functioning of the brain, including oxidative metabolism, synaptic plasticity, myelination, and generation of neurotransmitters. Dyshomeostasis of these redox-active metal ions in the brain could cause Alzheimer’s disease (AD). Thus, regulating the levels of Cu(I/II) and Fe(II/III) is necessary for normal brain function. To control the amounts of metal ions in the brain and understand the involvement of Cu(I/II) and Fe(II/III) in the pathogenesis of AD, many chemical agents have been developed. In addition, since toxic aggregates of amyloid-β (Aβ) have been proposed as one of the major causes of the disease, the mechanism of clearing Aβ is also required to be investigated to reveal the etiology of AD clearly. Multiple metalloenzymes (e.g., neprilysin, insulin-degrading enzyme, and ADAM10) have been reported to have an important role in the degradation of Aβ in the brain. These amyloid degrading enzymes (ADE) could interact with redox-active metal ions and affect the pathogenesis of AD. In this review, we introduce and summarize the roles, distributions, and transportations of Cu(I/II) and Fe(II/III), along with previously invented chelators, and the structures and functions of ADE in the brain, as well as their interrelationships.

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“…We identified a 489-amino acid protein (SCN18_10_11_15_R3_B_scaffold_20_388) that was initially annotated as thiocyanate hydrolase, but the three subunits expected (scnABC of the carbonyl sulfide pathway) were not present. Further analyses (Methods) indicated that the gene encodes thiocyanate desulfurase of the cyanate pathway (Tikhonova et al, 2020). This identification was strongly supported by selection of threading templates using I-TASSER (Yang et al, 2015) as well as by SWISS-MODEL (Waterhouse et al, 2018) (33.7% identity, top match to PDB 5F75, with four Cu ligand sites identified although the GMQE score is below expectation relative to the value expected for experimental structures of similar size) and Phyre2 (Kelley et al, 2015) (template fold library ID c5f30B, thiocyanate desulfurase from Thioalkalivibrio paradoxus) (Figure 4).…”

Section: Predicted Metabolism and Capacity For Scn − Degradationmentioning

confidence: 99%

Thiocyanate and Organic Carbon Inputs Drive Convergent Selection for Specific Autotrophic Afipia and Thiobacillus Strains Within Complex Microbiomes

et al. 2021

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Thiocyanate (SCN–) contamination threatens aquatic ecosystems and pollutes vital freshwater supplies. SCN–-degrading microbial consortia are commercially adapted for remediation, but the impact of organic amendments on selection within SCN–-degrading microbial communities has not been investigated. Here, we tested whether specific strains capable of degrading SCN– could be reproducibly selected for based on SCN– loading and the presence or absence of added organic carbon. Complex microbial communities derived from those used to treat SCN–-contaminated water were exposed to systematically increased input SCN concentrations in molasses-amended and -unamended reactors and in reactors switched to unamended conditions after establishing the active SCN–-degrading consortium. Five experiments were conducted over 790 days, and genome-resolved metagenomics was used to resolve community composition at the strain level. A single Thiobacillus strain proliferated in all reactors at high loadings. Despite the presence of many Rhizobiales strains, a single Afipia variant dominated the molasses-free reactor at moderately high loadings. This strain is predicted to break down SCN– using a novel thiocyanate desulfurase, oxidize resulting reduced sulfur, degrade product cyanate to ammonia and CO2 via cyanate hydratase, and fix CO2 via the Calvin–Benson–Bassham cycle. Removal of molasses from input feed solutions reproducibly led to dominance of this strain. Although sustained by autotrophy, reactors without molasses did not stably degrade SCN– at high loading rates, perhaps due to loss of biofilm-associated niche diversity. Overall, convergence in environmental conditions led to convergence in the strain composition, although reactor history also impacted the trajectory of community compositional change.

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Trinuclear copper biocatalytic center forms an active site of thiocyanate dehydrogenase

Cited by 21 publications

References 48 publications

The effect of heavy metals on thiocyanate biodegradation by an autotrophic microbial consortium enriched from mine tailings

The effect of heavy metals on thiocyanate biodegradation by an autotrophic microbial consortium enriched from mine tailings

Redox-Active Metal Ions and Amyloid-Degrading Enzymes in Alzheimer’s Disease

Thiocyanate and Organic Carbon Inputs Drive Convergent Selection for Specific Autotrophic Afipia and Thiobacillus Strains Within Complex Microbiomes

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