“… T. tenax cells (ATCC 30207; ATCC, Manassas, VA, USA) were cultured in Diamond’s medium as described previously ( 9 – 12 ). Briefly, they were incubated at 35°C for 5 days in a round-bottomed Falcon 14-mL tube (Fisher Scientific, Ottawa, Ontario, Canada) filled with 13 mL Diamond’s medium supplemented with 10% horse serum (Sigma-Aldrich, St. Louis, MO, USA) plus 100 U/mL ampicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).…”