Tritrichomonas foetus isolates from cats and cattle show minor genetic differences in unrelated loci ITS-2 and EF-1α

Reinmann, Karin; Müller, Norbert; Kuhnert, Peter; Campero, Carlos Manuel; Leitsch, David; Heß, Michael; Henning, Klaus; Fort, Marcelo; Müller, Joachim; Gottstein, Bruno; Frey, Caroline F.

doi:10.1016/j.vetpar.2011.09.032

Cited by 53 publications

(50 citation statements)

References 27 publications

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“…Sequencing of T. foetus isolated in the present study GenBank accession no. KX267765 showed 100% identity to homologous sequences of T. foetus isolated from other domestic cats in USA, AF466749 (Levy et al 2003) and EU569309 (Stockdale et al 2007, Walden et al 2013; Switzerland, JN006994 (Reinmann et al 2012); French, JX960422 (Profizi et al 2013); Norway, HM856630 (Tysnes et al 2011) and EF165538 (Dahlgren et al 2007); and Australia, GU170216 ) and JX187000 (Slapeta et al 2012). Furthermore, revealed a single nucleotide polymorphism (T>C) when compared with T. foetus isolated from cattle, similar to the findings previously described .…”

Section: Resultssupporting

confidence: 82%

“…The transmission route, if the cattle for the cat or the opposite, is an issue discussed by some authors (Stockdale et al 2007, Stockdale et al 2008, Slapeta et al 2012, Reinmann et al 2012) and molecular studies still disagree about the genetic identity of T. foetus (Yao & Kӧster 2015). Until now, the genetic sequence isolated in the present study may be classified as "cat genotype" like suggested by Slapeta et al (2010), because this sequence showed the same single nucleotide polymorphism (T> C) in the ITS2 region.…”

Section: Discussionmentioning

confidence: 55%

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Detection of natural occurrence of Tritrichomonas foetus in cats in Araçatuba, São Paulo, Brazil

et al. 2018

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RESUMO.-[Detecção da ocorrência natural deThe aim of this study was to investigate the occurrence of Tritrichomonas foetus in cats in the area surrounding the city of Araçatuba municipality, State of São Paulo, Brazil. Fecal samples from 129 cats were collected by rectal flush technique. It was compared two diagnosis methods, direct examination of feces and PCR. The presence of T. foetus DNA was verified using PCR by amplification of 347-bp fragment from the primers TFR3 and TFR4 and amplicons of positive cases were sequenced. Statistical analyses were performed investigating the associations between T. foetus infection with gender, age, breed, presence of diarrhea and/or history of diarrhea, previous treatment, lifestyle, origin, environment, and co-infection. T. foetus was observed in one sample (n=129) by direct microscopic examination of feces while PCR was positive in five samples (3.9%). Giardia species and Cryptosporidium species co-infection was also observed. Statistical analyses showed no significant associations between T. foetus infection and all listed factors, although most positive cats were asymptomatic and lived in multi-cat household. The isolates of T. foetus showed 100% identical sequences with other T. foetus isolates from cats around the world. So, the occurrence of T. foetus was confirmed in cats in Araçatuba city (Brazil). This parasite must be considered as a differential diagnosis in cats with diarrhea and also in asymptomatic carriers as source of infection in multi-cat environments.

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Section: Resultssupporting

confidence: 82%

Section: Discussionmentioning

confidence: 55%

Detection of natural occurrence of Tritrichomonas foetus in cats in Araçatuba, São Paulo, Brazil

et al. 2018

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“…However, recent studies unambiguously demonstrated that feline and bovine T. foetus exhibit conserved genetic differences (Reinmann et al, 2012;Slapeta et al, 2010Slapeta et al, , 2012Sun et al, 2012), thus suggesting different host tropism (Slapeta et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Tritrichomonas foetus: Prevalence study in naturally mating bulls in Switzerland

Bernasconi

Bodmer

Doherr

et al. 2014

Veterinary Parasitology

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Switzerland is officially free from bovine Tritrichomonas foetus. While bulls used for artificial insemination (AI) are routinely examined for this pathogen, bulls engaged in natural mating, as well as aborted fetuses, are only very sporadically investigated, indicating that the disease awareness for bovine tritrichomoniasis is low. Natural mating in cattle is becoming increasingly popular in Switzerland. Accordingly, a re-introduction/re-occurrence of T. foetus in cattle seems possible either via resurgence from a yet unknown bovine reservoir, or via importation of infected cattle. The low disease awareness for bovine tritrichomoniasis might favor an unnoticed re-establishment of T. foetus in the Swiss cattle population. The aim of our study was thus to search for the parasite, and if found, to assess the prevalence of bovine T. foetus in Switzerland. We included (1) bulls over two years of age used in natural mating and sent to slaughter, (2) bulls used for natural service in herds with or without fertility problems and (3) aborted fetuses. Furthermore, the routinely examined bulls used for AI (4) were included in this study. In total, 1362 preputial samples from bulls and 60 abomasal fluid samples of aborted fetuses were analyzed for the presence of T. foetus by both in vitro cultivation and molecular analyses. The parasite could not be detected in any of the samples, indicating that the maximal prevalence possibly missed was about 0.3% (95% confidence). Interestingly, in preputial samples of three bulls of category 1, apathogenic Tetratrichomonas sp. was identified, documenting a proof-of-principle for the methodology used in this study.

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“…1). Within the ITS2 region, band-tailed pigeon tritrichomonad isolates shared a single nucleotide polymorphism with published feline tritrichomonad isolates including NCSU Tfs-1 (AF644749) and T. blagburni isolates AUTf 1-7 and AUTf 9-13 (EU569301-EU569312) that distinguish them from tritrichomonad isolates from other host species including bovine T. foetus (JN105456), T. suis (JN006998) and T. mobilensis (TMU86612) (Reinmann et al, 2012) (Fig. 1).…”

Section: Birds Submitted By Wildlife Rehabilitation Centersmentioning

confidence: 94%

Dual-pathogen etiology of avian trichomonosis in a declining band-tailed pigeon population

Girard

Rogers

Woods

et al. 2014

Infection, Genetics and Evolution

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b s t r a c tThe Pacific Coast band-tailed pigeon (Patagioenas fasciata monilis) is a migratory game bird of North America that is at risk for population decline. Epidemics of avian trichomonosis caused by upper digestive tract infection with Trichomonas spp. protozoa in these and other doves and pigeons of the United States are sporadic, but can involve tens of thousands of birds in a single event. Herein, we analyze the role of trichomonosis in band-tailed pigeon mortality and relate spatial, temporal and demographic patterns of parasite transmission to the genetic background of the infecting organism. Infections were most common in adult birds and prevalence was high in band-tailed pigeons sampled at mortality events (96%) and rehabilitation centers (36%) compared to those that were hunter-killed (11%) or live-caught (4%). During non-epidemic periods, animals were primarily infected with T. gallinae Fe-hydrogenase subtype A2, and were less often infected with either T. gallinae subtype A1 (the British finch epidemic strain), T. stableri n. sp. (a T. vaginalis-like species), or Tritrichomonas blagburni n. sp.-like organisms. Birds sampled during multiple epidemics in California were only infected with T. gallinae subtype A2 and T. stableri. The non-clonal etiology of avian trichomonosis outbreaks in band-tailed pigeons and the risk of spill-over to raptor and passerine species highlights the need for additional studies that clarify the host range and evolutionary relationships between strains of Trichomonas spp. in regions of trichomonosis endemicity.

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Tritrichomonas foetus isolates from cats and cattle show minor genetic differences in unrelated loci ITS-2 and EF-1α

Cited by 53 publications

References 27 publications

Detection of natural occurrence of Tritrichomonas foetus in cats in Araçatuba, São Paulo, Brazil

Detection of natural occurrence of Tritrichomonas foetus in cats in Araçatuba, São Paulo, Brazil

Tritrichomonas foetus: Prevalence study in naturally mating bulls in Switzerland

Dual-pathogen etiology of avian trichomonosis in a declining band-tailed pigeon population

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