Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells

Ribeiro, Ana R.; Gemini-Piperni, Sara; Travassos, Renata; Lemgruber, Leandro; Silva, R. C.; Rossi, André L.; Farina, Marcos; Anselme, Karine; Shokuhfar, Tolou; Shahbazian‐Yassar, Reza; Borojević, Radovan; Rocha, L. A.; Werckmann, J.; Granjeiro, José Mauro

doi:10.1038/srep23615

Cited by 87 publications

(103 citation statements)

References 48 publications

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“…Gold NPs have been shown as an effective material with no osteogenesis potential, nor apoptosis to osteoblasts (the MG63 cell line) (Tsai et al 2013). Conversely, the anatase titanium dioxide (the metallic implant debris-originated) was internalized readily by primary osteoblasts, and affected the adhesion strength, migration and proliferation of the cells negatively (Ribeiro et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

et al. 2017

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Covalent conjugation of (bis)phosphonate group-containing molecules, sodium Alendronate (Aln) and 3-AminoropylPhosphoric Acid (ApA), to Cellulose nanocrystals (CNCs) was performed via oxidation/Shiff-base reaction. Further fluorescent labelling with Rhodamine B Iso ThioCyanate (RBITC) was performed to follow CNCs interaction and potential internalization with/in human osteoblasts by confocal microscopy. Complementary analyses were applied to identify the conjugation (Atenuated Total Reflectance-Fourier Transform Infrared and UV-VIS spectroscopies), physico-chemical (Dynamic Light Scattering and Nanoparticle Tracking Analysis) and morphological (Transmission Electron Microscopy) features of native and ApA/Aln-modified CNCs in physiologically relevant environments (Phosphate Buffer Saline, Advanced Dulbecco's Modified Eagle Medium). While conjugation did not affect the CNCs' size, the RBITC-labelling promotes their aggregation. Faster (1 h vs. 2 h) uptake by osteoblasts of RBITCCNCoxAln, compared to RBITC-CNCoxApA, and no-internalization (in 24 h) of native RBITTC-CNC, indicate a higher affinity of Aln-modified CNCs to the cells, while all CNCs (in 0.25-0.06 wt%) promote the cell growth. Aln/Apa-modified CNCs shows high potential in drug-delivery for bone therapies, and theranostics.

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Section: Introductionmentioning

confidence: 99%

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

et al. 2017

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“…Only a concentration of 9 ppm increased the expression of RANKL mRNA in osteoblasts and not in epithelial cells. A recent study investigated the nano-bio interactions in a biological environment and the process of nanoparticle internalization by cells (Ribeiro et al, 2016). The filtration of a titanium solution (pore size 0.22 μm) deleted the effect on macrophages but did not lower cytotoxicity at higher concentration.…”

Section: Bonementioning

confidence: 99%

What is the impact of titanium particles and biocorrosion on implant survival and complications? A critical review

Mombelli

Hashim

2018

Clinical Oral Implants Res

165

137

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Objectives To compile the current evidence regarding the association between the release of titanium particles and biologic complications of dental implants. Material and methods This is a critical review. We searched the literature using the terms “corrosion,” “allergy,” “hypersensitivity,” or “particles” together with “titanium,” “Ti,” “TiO2.” The bibliographies of identified publications and previously published review articles were scanned to find additional related articles. We included clinical studies, in vivo and in vitro experiments. Results Titanium particles and degradation products of titanium have been detected in oral and nonoral tissues. Particles are released from surfaces of dental implants because of material degradation in a process called tribocorrosion. It involves mechanical wear and environmental factors, notably contact to chemical agents and interaction with substances produced by adherent biofilm and inflammatory cells. In vitro, titanium particles can interfere with cell function and promote inflammation. A temporal association between exposure to titanium and occurrence of tissue reactions suggested hypersensitivity in a limited number of cases. However, there is poor specificity as the observed reactions could be initiated by other factors associated with the placement of implants. Titanium particles are commonly detected in healthy and diseased peri‐implant mucosa alike, at low levels even in gingiva of individuals without titanium implants. Rather than being the trigger of disease, higher concentrations of titanium in peri‐implantitis lesions could be the consequence of the presence of biofilms and inflammation. Conclusion There is an association between biocorrosion, presence of titanium particles, and biological implant complications, but there is insufficient evidence to prove a unidirectional causal relationship.

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“…Such a bio‐complex works as a kind of “Trojan horse” that facilitates nanoparticle internalization by cells. Thereafter, nanoparticles can interact with internal cell organelles and structures, leading to functional modifications and intracellular lesions …”

Section: Peri‐implantitis Induced By Corrosion and Wearmentioning

confidence: 99%

Synergistic interactions between corrosion and wear at titanium‐based dental implant connections: A scoping review

Apaza‐Bedoya

Tarce

Benfatti

et al. 2017

J of Periodontal Research

122

110

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Two-piece implant systems are mainly used in oral implantology involving an osseointegrated implant connected to an abutment, which supports prosthetic structures. It is well documented that the presence of microgaps, biofilms and oral fluids at the implant-abutment connection can cause mechanical and biological complications. The aim of this review paper was to report the degradation at the implant-abutment connection by wear and corrosion processes taking place in the oral cavity. Most of the retrieved studies evaluated the wear and corrosion (tribocorrosion) of titanium-based materials used for implants and abutments in artificial saliva. Electrochemical and wear tests together with microscopic techniques were applied to validate the tribocorrosion behavior of the surfaces. A few studies inspected the wear on the inner surfaces of the implant connection as a result of fatigue or removal of abutments. The studies reported increased microgaps after fatigue tests. In addition, data suggest that micromovements occurring at the contacting surfaces can increase the wear of the inner surfaces of the connection. Biofilms and/or glycoproteins act as lubricants, although they can also amplify the corrosion of the surfaces. Consequently, loosening of the implant-abutment connection can take place during mastication. In addition, wear and corrosion debris such as ions and micro- and nanoparticles released into the surrounding tissues can stimulate peri-implant inflammation that can lead to pathologic bone resorption.

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Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells

Cited by 87 publications

References 48 publications

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

What is the impact of titanium particles and biocorrosion on implant survival and complications? A critical review

Synergistic interactions between corrosion and wear at titanium‐based dental implant connections: A scoping review

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