Trophic coupling of a testate amoeba and Microcystis species in a hypertrophic pond

Nishibe, Yuichiro; Manage, P.M.; Kawabata, Z.; Nakano, Susumu

doi:10.1007/s10201-004-0114-9

Cited by 24 publications

(25 citation statements)

References 22 publications

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“…Lansac-Tôha et al 2009). However, our results indicate that this group is a poor indicator of eutrophication (but see Branco et al 2002;Nishibe et al 2004). The inclusion of this group in biomonitoring programs is, probably, only justified to evaluate the impacts derived from hydrological changes caused by river damming (Lansac-Tôha et al 2009).…”

Section: Discussionmentioning

confidence: 79%

Zooplankton Community Metrics as Indicators of Eutrophication in Urban Lakes

Lodi¹,

Vieira²,

Velho³

et al. 2011

NatCon

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Although the direct effects of eutrophication are well known, its indirect effects are poorly understood and the interaction with non-nutrient factors may alter some expected relationships. We analyzed the reliability of community-level metrics derived from three zooplankton groups as predictors of eutrophication in urban man-made lakes. Univariate and multivariate correlation analyses were used to test for relationships between environmental variables and community metrics derived from zooplankton data. Our results indicated that rotifer community metrics were the best eutrophication indicators. The main implication of our results is that arguments against the use of simple community-level metrics as indicators of eutrophication cannot be generalized. Our findings also suggest the need of complete sample analyses (i.e., identification and counting) to estimate reliable ecological indicators of eutrophication.

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Section: Discussionmentioning

confidence: 79%

Zooplankton Community Metrics as Indicators of Eutrophication in Urban Lakes

Lodi¹,

Vieira²,

Velho³

et al. 2011

NatCon

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“…Previous laboratory studies have demonstrated that some species of flagellates, including the heterotrophic flagellate Collodictyon triciliatum and the mixotrophic flagellate Poterioochromonas sp. (Nishibe et al 2002, Ou et al 2005, and rhizopods (Nishibe et al 2004) graze on Microcystis populations. While amoeboid grazing is relatively well understood there is less information regarding flagellate grazing on Microcystis populations.…”

Section: Introductionmentioning

confidence: 99%

Grazing and growth of the heterotrophic flagellate Diphylleia rotans on the cyanobacterium Microcystis aeruginosa

Kim¹,

Nakano²,

Kim³

et al. 2006

Aquat. Microb. Ecol.

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) by D. rotans were observed when they were fed the most toxic of the 5 Microcystis strains, M. aeruginosa NIER-10001. These results suggest that D. rotans has a high tolerance to M. aeruginosa and may undergo microcystin-mediated growth.KEY WORDS: Algivores · Heterotrophic flagellates · Diphylleia rotans · Microcystis spp. · Microcystin-mediated growth · Ingestion Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 45: [163][164][165][166][167][168][169][170] 2006 examined the ecophysiology of this flagellate on its prey using comparisons of grazing behavior on toxic and non-toxic Microcystis strains, along with other algal strains, which represent different food types and qualities. MATERIALS AND METHODSIsolation and culture of the heterotrophic flagellate. Furuike Pond (33°49' 21'' N, 132°48' 04'' E; altitude 40 m) is an impoundment located in Sancho, Matsuyama City, Ehime Prefecture, Japan. It has a surface area of 7400 m 2 and a maximum depth of 1.5 m. The pond is hypereutrophic due to anthropogenic loading from the watershed (Nakano et al. 2001b) and is heavily populated by cyanobacteria, such as Microcystis aeruginosa and M. wesenbergii, from early summer to fall each year (Manage et al. 1999).For isolation of a flagellate capable of grazing on Microcystis species, Microcystis aeruginosa NIES-298 was incubated in Cytophaga broth (CB) medium (MCC-NIES; www.nies.go.jp/biology/mcc/home_j.htm) and mixed with a pond water sample that had been filtered through a Nitex net (mesh size, 154 µm) for removal of larger zooplankton. This mixture was incubated at 25°C in the dark for 3 to 4 d. Flagellates were isolated using a Pasteur micropipette under a light microscope. We established a monoclonal flagellate culture using a repetitive serial isolation process consisting of enrichment, dilution and single cell isolation steps. The isolated flagellate was maintained using a commercial strain of Chlorella TM (Aquanet) as prey. Water was collected from the Pal'tang Reservoir, Korea, filtered through a Whatman GF/F filter and sterilized in an autoclave to yield filtered sterilized water (FSW). A quantity of Chlorella TM was suspended in the FSW, and the flagellate was inoculated into the Chlorella TM suspension. We maintained the flagellate strain by inoculating it weekly into new Chlorella TM suspensions. Incubations were performed at 25°C under a light intensity of 40 to 45 µE m -2 s -1 of cool white fluorescent light, with a 12:12 h light:dark photoperiod.To identify the flagellate, we used light and epifluorescence microscopy and 18S rDNA sequencing. Morphological observations, including the presence of 2 long apical and equal flagella inserting near the anterior end at the top of the ventral groove formed by the curving lateral margins of the cell, a cell dimension of 15 × 20 µm (subject to change during feeding), and feeding observations such as free movement or rotation during feeding via the ventral groove, identified the flagellate as Diphylleia rotans (Massart 1920...

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“…Previous studies have reported as possible grazers of Microcystis: protists (Cole and Wynne 1974;Dryden and Wright 1987), rotifers (Snell 1980;Fulton and Paerl 1987), crustacean zooplankton (Hanazato and Yasuno 1984;Jarvis et al 1987), and fish (Moriarty 1973;Kawanabe and Mizuno 1989;Miura 1990). There are only a limited number of rotifers, crustaceans, and fish that graze on Microcystis, but various protistan species have been shown to do so (Zhang et al 1996;Nishibe et al 2002Nishibe et al , 2004Kim et al 2006;Wilken et al 2010). Thus, it is possible that the wax and wane of a Microcystis bloom are dependent on grazing by protists.…”

Section: Introductionmentioning

confidence: 92%

“…Among such protistan grazers, rhizopods, including both naked and testate amoebae, are frequently found to be abundant when significant decreases in Microcystis abundance are detected in lakes, and grazing on Microcystis by some rhizopod species has been demonstrated in laboratory experiments (Yamamoto 1981;Yamamoto and Suzuki 1984;Nishibe et al 2004) and field observations (Whitton 1973;Nishibe et al 2004). Unfortunately, we still have limited eco-physiological information about the rhizopods that graze on Microcystis.…”

Section: Introductionmentioning

confidence: 96%

“…Rodriguez-Zaragoza (1994) has reported that excessive nutrients and elevated water temperatures may be beneficial to common rhizopod species because such environmental conditions favor bacterial growth, and the bacteria in turn feed rhizopods. Nishibe et al (2004) reported that the abundance of the testate amoebae Penardochlamys sp., which grazes on Microcystis, was high when Microcystis was abundant in a hypereutrophic pond. High rhizopod abundance with high Microcystis abundance may be reasonable, since the relationship of consumption by a grazer on various densities of prey follows the Michaelis-Menten equation.…”

Section: Introductionmentioning

confidence: 99%

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Grazing on Microcystis (Cyanophyceae) by testate amoebae with special reference to cyanobacterial abundance and physiological state

et al. 2010

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We examined the growth of testate amoebae preying on Microcystis whose physiological states were different in laboratory experiments and a hypertrophic pond. We prepared three experimental systems using water samples dominated by Microcystis aeruginosa: light incubation (control), dark incubation (dark), and light incubation with addition of nitrogen and phosphorus (?NP). In all the systems, the colony density of M. aeruginosa decreased slightly during incubation. Physiological activity of phytoplankton as determined by chlorophyll fluorescence was high and almost constant in the control and ?NP systems, whereas it decreased in the dark system. Cell densities of testate amoebae increased in the control and ?NP systems, whereas in the dark system they remained low. Thus, growth of the amoebae was low in the systems where physiological activity of Microcystis was low. In a hypertrophic pond, cell density of testate amoebae increased and remained high when M. aeruginosa predominated. Cell density of testate amoebae increased remarkably, simultaneously with the increases in M. aeruginosa colony density and phytoplankton physiological activity. We also found a significant correlation between densities of M. aeruginosa colonies and testate amoebae. We suggested that the physiological activity of Microcystis is one important factor affecting the growth of testate amoebae grazing on Microcystis.

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Trophic coupling of a testate amoeba and Microcystis species in a hypertrophic pond

Cited by 24 publications

References 22 publications

Zooplankton Community Metrics as Indicators of Eutrophication in Urban Lakes

Zooplankton Community Metrics as Indicators of Eutrophication in Urban Lakes

Grazing and growth of the heterotrophic flagellate Diphylleia rotans on the cyanobacterium Microcystis aeruginosa

Grazing on Microcystis (Cyanophyceae) by testate amoebae with special reference to cyanobacterial abundance and physiological state

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