TRPV2-immunoreactive intrinsic neurons in the rat intestine

Kashiba, Hitoshi; Uchida, Yuzo; Takeda, Daisuke; Nishigori, Ayahiko; Ueda, Yoshihiko; Kuribayashi, Koichi; Ohshima, Minoru

doi:10.1016/j.neulet.2004.05.069

Cited by 34 publications

(20 citation statements)

References 15 publications

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“…2 B). Similar to the rat intestine (Kashiba et al, 2004), TRPV2-immunoreactive (IR) cells costained with the neuron marker PGP9.5 (Fig. 2 A).…”

Section: Expression Of Trpv2 Protein In Intestine Myenteric Neuronsmentioning

confidence: 76%

“…Because TRPV2 was shown to be expressed in the rat myenteric plexus and to have some mechanosensitivity (Muraki et al, 2003;Kashiba et al, 2004;Shibasaki et al, 2010), we examined TRPV2 expression in the small intestine. mRNA expression of TRPV2 and the control PGP9.5 (a neuron marker) was detected in whole small intestine, longitudinal muscle with myenteric plexus (LMMP), and intestinal MCC (Fig.…”

Section: Expression Of Trpv2 Mrna In Intestine Myenteric Neuronsmentioning

confidence: 99%

“…TRPV2 expression has been observed in TRPV1-negative medium-to large-diameter neurons of the rat dorsal root and trigeminal ganglia and possibly in thinly myelinated nociceptors (Caterina et al, 1999;Lewinter et al, 2008). However, TRPV2 mRNA or immunoreactivity has also been detected in the brain, autonomic ganglia, spinal cord, skeletal and vascular myocytes, visceral organs (including the intestine, pancreas, spleen, and bladder), and blood cells (Caterina et al, 1999;Ichikawa and Sugimoto, 2001;Muraki et al, 2003;Kashiba et al, 2004;Lewinter et al, 2004;Inada et al, 2006;Lewinter et al, 2008;Hisanaga et al, 2009;Zanou et al, 2009;Link et al, 2010). TRPV2 expression may also be involved in the migration and proliferation of cancer cells (Caprodossi et al, 2008;Monet et al, 2010), and TRPV2-positive neurons observed in the rat myenteric plexus may be IPANs (Kashiba et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…However, TRPV2 mRNA or immunoreactivity has also been detected in the brain, autonomic ganglia, spinal cord, skeletal and vascular myocytes, visceral organs (including the intestine, pancreas, spleen, and bladder), and blood cells (Caterina et al, 1999;Ichikawa and Sugimoto, 2001;Muraki et al, 2003;Kashiba et al, 2004;Lewinter et al, 2004;Inada et al, 2006;Lewinter et al, 2008;Hisanaga et al, 2009;Zanou et al, 2009;Link et al, 2010). TRPV2 expression may also be involved in the migration and proliferation of cancer cells (Caprodossi et al, 2008;Monet et al, 2010), and TRPV2-positive neurons observed in the rat myenteric plexus may be IPANs (Kashiba et al, 2004). Mechanosensitivity of TRPV2 was first reported in cardiac myocytes (Muraki et al, 2003), and we have recently reported its expression and mechanosensitivity in developing sensory and motor neurons (Shibasaki et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Involvement of TRPV2 Activation in Intestinal Movement through Nitric Oxide Production in Mice

Mihara¹,

Boudaka²,

Shibasaki³

et al. 2010

J. Neurosci.

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Transient receptor potential channel vanilloid 2 (TRPV2) can detect various stimuli such as temperature (Ͼ52°C), stretch, and chemicals, including 2-aminoethoxydiphenyl borate, probenecid, and lysophospholipids. Although expressed in many tissues, including sensory and motor neurons, TRPV2 expression and function in the gastrointestinal tract is poorly understood. Here, we show TRPV2 expression in the murine intestine and its involvement in intestinal function. Almost all mouse intestinal intrinsic sensory and inhibitory motor neurons, both cell bodies and nerve fibers, showed TRPV2 immunoreactivity. Several known TRPV2 activators increased cytosolic Ca 2ϩ concentrations and evoked TRPV2-like current responses in dissociated myenteric neurons. Interestingly, mechanical stimuli activated inward currents in a strength-dependent manner, which were inhibited by a TRPV2 inhibitor tranilast. TRPV2 activation in isolated intestine inhibited spontaneous circular muscle contraction, which did not occur in the presence of the TRPV2 antagonist, tetrodotoxin or nitro oxide (NO) synthase pathway inhibitors. Also, increased intestinal NO production was observed in response to a TRPV2 agonist, and gastrointestinal transit in vivo was accelerated by TRPV2 agonists or an NO donor. In conclusion, TRPV2 may contribute to intestinal motility through NO production, and TRPV2 is a promising target for controlling intestinal movement.

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“…2 B). Similar to the rat intestine (Kashiba et al, 2004), TRPV2-immunoreactive (IR) cells costained with the neuron marker PGP9.5 (Fig. 2 A).…”

Section: Expression Of Trpv2 Protein In Intestine Myenteric Neuronsmentioning

confidence: 76%

Section: Expression Of Trpv2 Mrna In Intestine Myenteric Neuronsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Involvement of TRPV2 Activation in Intestinal Movement through Nitric Oxide Production in Mice

Mihara¹,

Boudaka²,

Shibasaki³

et al. 2010

J. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Collectively, these data suggest a potential role of TRPV2 in pain sensation. However, compared with TRPV1, TRPV2 has a wider distribution pattern, including brain, skin, spleen, lung, stomach, intestines, bladder, prostate, and peripheral blood (Caterina et al, 1999;Kowase et al, 2002;Kashiba et al, 2004;Saunders et al, 2007;Caprodossi et al, 2008), suggesting that TRPV2 may subserve a relatively broad repertoire of physiological functions. In addition, species-dependent activation of TRPV2 in response to noxious heat (ϳ53°C) and to the nonselective TRP agonist 2-aminoethoxydiphenyl borate (2-APB) was reported recently (Juvin et al, 2007;Neeper et al, 2007), indicating that the gating mechanisms and the role of this channel in vivo are likely more complicated than were first presumed.…”

Section: Introductionmentioning

confidence: 99%

TRPV2 Is Activated by Cannabidiol and Mediates CGRP Release in Cultured Rat Dorsal Root Ganglion Neurons

Qin¹,

Neeper²,

Liu³

et al. 2008

Journal of Neuroscience

341

290

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Transient receptor potential V2 (TRPV2) has been proposed to be a high-threshold thermosensor. However, further elucidation of the channel properties and physiological role of TRPV2 have been hindered by the lack of selective pharmacological tools as well as by the species-dependent differences in the activation of this channel. In the present study, we have used cell-based calcium mobilization and electrophysiological assays to identify and characterize several novel cannabinoid TRPV2 agonists. Among these, cannabidiol was found to be the most robust and potent (EC 50 ϭ 3.7 M), followed by ⌬ 9 -tetrahydrocannabinol (EC 50 ϭ 14 M) and cannabinol (EC 50 ϭ 77.7 M). We also demonstrated that cannabidiol evoked a concentration-dependent release of calcitonin gene-related peptide (CGRP) from cultured rat dorsal root ganglion neurons in a cannabinoid receptor-and TRPV1-independent manner. Moreover, the cannabidiolevoked CGRP release depended on extracellular calcium and was blocked by the nonselective TRP channel blocker, ruthenium red. We further provide evidence through the use of small interfering RNA knockdown and repetitive stimulation studies, to show that cannabidiol-evoked CGRP release is mediated, at least in part, by TRPV2. Together, these data suggest not only that TRPV2 may comprise a mechanism whereby cannabidiol exerts its clinically beneficial effects in vivo, but also that TRPV2 may constitute a viable, new drug target.

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TRP Channels in the Brain

Reboreda

2012

Methods in Pharmacology and Toxicology

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TRPV2-immunoreactive intrinsic neurons in the rat intestine

Cited by 34 publications

References 15 publications

Involvement of TRPV2 Activation in Intestinal Movement through Nitric Oxide Production in Mice

Involvement of TRPV2 Activation in Intestinal Movement through Nitric Oxide Production in Mice

TRPV2 Is Activated by Cannabidiol and Mediates CGRP Release in Cultured Rat Dorsal Root Ganglion Neurons

TRP Channels in the Brain

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