Trueperella pyogenes isolated from a brain abscess of an adult roebuck (Capreolus capreolus)

Abstract

The present study was designed to characterize phenotypically and genotypically a Trueperella pyogenes strain isolated from a brain abscess of an adult roebuck (Capreolus capreolus). The species identity could be confirmed by phenotypical investigations, by MALDI-TOF MS analysis, and by sequencing the 16S ribosomal RNA (rRNA) gene, the 16S-23S rRNA intergenic spacer region (ISR); by sequencing the target genes rpoB, gap, and tuf; and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. The T. pyogenes strain could additionally be characterized by PCR-mediated amplification of several known and putative virulence factor-encoding genes which revealed the presence of the genes plo encoding pyolysin and nanH and nanP encoding neuraminidases; the genes fimA, fimC, and fimE encoding the fimbrial subunits FimA, FimC, and FimE; and the gene cbpA encoding collagen-binding protein CbpA. The present data give a detailed characterization of a T. pyogenes strain isolated from a brain abscess of a roebuck. However, the route of infection of the roebuck remains unclear.

Studies on Trueperella Pyogenes Isolated from an Okapi (Okapia johnstoni) and A Royal Python (Python Regius)

“…The previously described cpn60-speci c LAMP assay could successfully be used to identify the speciesspeci c gene cpn60 of T. pyogenes 24398 and T. pyogenes 171003246 in the present investigation. This was comparable to the LAMP assay for detecting gene cpn60 of the previously described T. pyogenes of various origins (25), a T. pyogenes strain isolated from an adult roebuck (Capreolus capreolus) (23), and a T. pyogenes strain isolated from a eurasian lynx (Lynx lynx) (20). The results of the cpn60 LAMP assay are shown in Fig.…”

Studies on Trueperella pyogenes isolated from an okapi (Okapia johnstoni) and a royal python (Python regius)

“…The previously described cpn60-specific LAMP assay could successfully be used to identify the species-specific gene cpn60 of T. pyogenes 24,398 and T. pyogenes 171, 003,246 in the present investigation. This was comparable to the LAMP assay for detecting gene cpn60 of the previously described T. pyogenes of various origins [24], a T. pyogenes strain isolated from an adult roebuck (Capreolus capreolus) [23], and a T. pyogenes strain isolated from a eurasian lynx (Lynx lynx) [20]. The results of the cpn60 LAMP assay are shown in Fig.…”

“…Both T. pyogenes isolates were also evaluated by PCR for the presence of five genomic targets: 16S rRNA gene (16S), 16S-23S rDNA intergenic spacer region (ISR), the β-subunit of bacterial RNA polymerase encoding gene rpo B, the elongation factor tu encoding gene tuf , and pyolysin encoding gene plo . The sequence of the oligonucleotide primers and PCR conditions were previously described by Hassan et al [ 25 ], Ülbegi-Mohyla et al [ 12 ], Hijazin et al [ 27 ], Eisenberg et al [ 18 ], Wickhorst et al [ 28 ], Wickhorst et al [ 23 ], Alssahen et al [ 20 ].…”

“…Besides conventional bacteriological methods for identifying T. pyogenes isolates, other new, fast and reliable techniques were described and utilized in this study: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) [ 20 – 23 ], a loop-mediated isothermal amplification (LAMP) assay [ 24 ] and 16S rRNA gene sequencing [ 25 , 26 ].…”

Determination of the expression of three fimbrial subunit proteins in cultured Trueperella pyogenes

“…Trueperella pyogenes is a Gram-positive rod-shaped bacterium [ 1 ]. This organism is a commensal and a common opportunistic pathogen in different domestic and wild animals [ 2 – 4 ]. Moreover, human beings can be infected by this bacterium; however, the infections are generally related to occupational exposure [ 5 ].…”