Trypanocidal activity of polysaccharide extract from Genipa americana leaves

Souza, Racquel Oliveira da Silva; Sousa, Paloma Leão; Menezes, Ramon Róseo Paula Pessoa Bezerra de; Sampaio, Tiago Lima; Tessarolo, Louise Donadello; Silva, Francisca Crislândia Oliveira; Pereira, Maria Lidiane de Sousa; Martins, Alice Maria Costa

doi:10.1016/j.jep.2017.08.042

Cited by 20 publications

(9 citation statements)

References 39 publications

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“…The leaves have anticonvulsive (Nonato et al, 2018), antimalarial (Deharo et al, 2001) and trypanocidal properties (Souza et al, 2018). Phenolic extracts of fruits have the ability to inhibit the proliferation of cells responsible for human hepatocarcinoma (Finco and Groeve, 2013).…”

Section: Introductionmentioning

confidence: 99%

Research Article Genetic diversity in native Genipa americana (Rubiaceae) populations in Sergipe, Brazil

Cardoso¹,

Nascimento²,

Oliveira³

et al. 2019

Genet. Mol. Res.

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Genipap (Genipa americana-Rubiaceae) is native to tropical America, occurring in Brazil in practically all biomes, except in the Pampas. It has socioeconomic importance, mainly due to the use of fruits in the manufacture of sweets and juices besides the medicinal properties of its leaves. We evaluated the genetic diversity of 73 individuals from 15 natural populations in the state of Sergipe using ISSR markers. The choice of areas was made randomly and genetic material was extracted from young leaves. PCR analysis using nine markers generated 113 fragments, which were used to estimate genetic diversity. The Shannon index was 0.36 and the similarity of individuals varied between 0.11 and 0.81. The individuals Salgado 3 (SAL3) and Salgado 4 (SAL4) were the most similar (0.81) and seven pairs had low similarity (0.11). The genetic distance the UPGMA analysis, which divided individuals into two distinct groups. The level of genetic variability found allows differentiation between genotypes that can be used to enrich the Genipap Active GermPlasm Bank in Sergipe, and the information generated will be useful for the conservation of these genetic resources and in future breeding programs for this species.

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Section: Introductionmentioning

confidence: 99%

Research Article Genetic diversity in native Genipa americana (Rubiaceae) populations in Sergipe, Brazil

Cardoso¹,

Nascimento²,

Oliveira³

et al. 2019

Genet. Mol. Res.

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“…[28] There is no previous study on the trypanocidal activity of this EO; however, the T. riparia methanol extract has been evaluated against T. cruzi trypomastigotes, T. brucei trypomastigotes, and L. infantum promastigotes, giving IC 50 values of 33.2, 8.1, and 20.3 μg mL À 1 , respectively. [29,30] It is important to determine the EO cytotoxic potential when searching for chemotherapeutic alternatives. [31] Natural products with CC 50 (concentration cytotoxic to 50 % of the cells) values lower than 100 μg mL À 1 , between 100 and 500 μg mL À 1 , between 500 and 1000 μg mL À 1 , and higher than 1000 μg mL À 1 are strongly cytotoxic, moderately cytotoxic, weakly cytotoxic, and not cytotoxic to mammalian cells, respectively.…”

Section: Resultsmentioning

confidence: 99%

Trypanocidal Activity of Dysphania ambrosioides, Lippia alba, and Tetradenia riparia Essential Oils against Trypanosoma cruzi

Crotti

Pagotti

Cândido

et al. 2021

Chemistry & Biodiversity

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Despite the current treatments against Chagas Disease (CD), this vector-borne parasitic disease remains a serious public health concern. In this study, we have explored the in vitro and/or in vivo trypanocidal and cytotoxic activities of the essential oils (EOs) obtained from Dysphania ambrosioides (L.) Mosyakin & Clemants (Amaranthaceae) (DA-EO), Lippia alba (Mill.) N.E. Brown (Verbenaceae) (LA-EO), and Tetradenia riparia (Hochst.) Codd (Lamiaceae) (TR-EO) grown in Brazil Southeast. DA-EO was the most active against the trypomastigote and amastigote forms in vitro; the IC 50 values were 8.7 and 12.2 μg mL À 1 , respectively. The EOs displayed moderate toxicity against LLCMK2 cells, but the DA-EO showed high selectivity index (SI) for trypomastigote (SI = 33.2) and amastigote (SI = 11.7) forms. Treatment with 20 mg/kg DA-EO, LA-EO, or TR-EO for 20 days by intraperitoneal administration reduced parasitemia by 6.36 %, 4.74 %, and 32.68 % on day 7 and by 12.04 %, 27.96 %, and 65.5 % on day 9. These results indicated that DA-EO, LA-EO, and TR-EO have promising trypanocidal potential in vitro, whereas TR-EO has also potential trypanocidal effects in vivo.

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“…The polysaccharide extract of G. americana showed activity against all main forms of a benznidazole-resistant T. cruzi strain, suggesting a necrotic pathway with ROS involvement. Therefore, GaEPL can be a source of substances against Chagas disease (Souza et al 2018). In this work, we evaluated two arabinogalactan-glycoconjugate fractions of this extract.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, we demonstrated trypanocidal action of a polysaccharide extract from G. americana leaves against a benznidazole-resistant strain of T. cruzi (Souza et al 2018). Two arabinogalactan-glycoconjugates from this extract were separated and characterized, being mainly composed of arabinose, galactose, and uronic acid.…”

Section: Introductionmentioning

confidence: 99%

Arabinogalactan-Glycoconjugate Fractions from Genipa americana Leaves as a Source of Antichagasic Natural Products

Souza

Sousa

Menezes

et al. 2020

Rev. Bras. Farmacogn.

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Trypanocidal activity of polysaccharide extract from Genipa americana leaves

Abstract: GaEPL showed significant activity against the epimastigote, trypomastigote and amastigote forms of T. cruzi, strain Y, suggesting cell death by necrosis with involvement of reactive oxygen species.

Cited by 20 publications

References 39 publications

Research Article Genetic diversity in native <i>Genipa</i> <i>americana</i> (Rubiaceae) populations in Sergipe, Brazil

Research Article Genetic diversity in native <i>Genipa</i> <i>americana</i> (Rubiaceae) populations in Sergipe, Brazil

Trypanocidal Activity of Dysphania ambrosioides, Lippia alba, and Tetradenia riparia Essential Oils against Trypanosoma cruzi

Arabinogalactan-Glycoconjugate Fractions from Genipa americana Leaves as a Source of Antichagasic Natural Products

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