Trypanosoma evansi: Immune response and acetylcholinesterase activity in lymphocytes from infected rats

Silva, Aleksandro S. Da; Monteiro, Sílvia González; Gonçalves, Jamile Fabbrin; Spanevello, Rosélia Maria; Schmatz, Roberta; Oliveira, Camila Belmonte; Costa, Márcio Machado; França, Raqueli Teresinha; Jaques, Jeandre A.; Schetinger, Maria Rosa Chitolina; Mazzanti, Cinthia M.; Lopes, Sonia T.A.

doi:10.1016/j.exppara.2010.10.017

Cited by 13 publications

(8 citation statements)

References 45 publications

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“…Similar results were observed in lymphocytes and brain of rats infected with T. evansi, which had activation of the enzyme AChE (Da Silva et al, 2011b;Da Silva et al, 2011c). This increase in the activity of AChE in total blood may be explained as an inflammatory response caused by the parasite as described in the literature (Vespa et al, 1994;Michailowsky et al, 2001).…”

Section: Discussionsupporting

confidence: 73%

“…As shown by researchers, the inflammatory mediators are produced during infection with T. cruzi (Vespa et al, 1994;Michailowsky et al, 2001). Therefore, it is possible to suggest that the inflammatory reaction triggered by the T. cruzi infection might lead to this increase in AChE activity and, as an outcome, a decrease in ACh, which possess anti-inflammatory characteristics, similar as T. evansi infection (Da Silva et al, 2011c).…”

Section: Discussionmentioning

confidence: 99%

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Activity of cholinesterases and adenosine deaminase in blood and serum of rats experimentally infected withTrypanosoma cruzi

Silva

Pimentel

Fiorenza

et al. 2011

Annals of Tropical Medicine & Parasitology

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This study aimed to evaluate the activity of cholinesterases and adenosine deaminase (ADA) in blood and serum of rats infected with Trypanosoma cruzi. Twelve adult rats were used in the experiment divided into two uniform groups. Rodents from group A (control group) were non-infected and animals from group B served as infected, receiving intraperitoneally 3.3610 7 trypomastigotes/each. Blood collection was performed at days 60 and 120 post-infection (PI) in order to evaluate the hemogram, blood activity of acetylcholinesterase, and serum butyrylcholinesterase and ADA activities. Hematological parameters did not differ between groups. A significant increase (P,0.05) of acetylcholinesterase activity was observed in blood while butyrylcholinesterase had a significant reduction (P,0.01) in serum of infected rats at days 60 and 120 PI. ADA activity in serum showed an inhibition in infected animals when compared to non-infected at day 120 PI. Based on these results, it is possible to conclude that the activity of cholinesterases and ADA were changed in animals infected with T. cruzi. The possible causes of these alterations will be discussed in this paper.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Activity of cholinesterases and adenosine deaminase in blood and serum of rats experimentally infected withTrypanosoma cruzi

Silva

Pimentel

Fiorenza

et al. 2011

Annals of Tropical Medicine & Parasitology

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“…For the practical purpose, the measurement of cholinergic status other than brain tissue is imperative. It was noted that serum ChE activity might be used in the diagnosis of stroke [ 14 ] and infection [ 30 , 31 ]. Although a significant decrease in serum ChE levels had been reported in human acute head injury [ 32 ], data regarding the association of ChE activity with blood cell counts, cognition, and neurofunctional outcome in TBI is extremely limited.…”

Section: Introductionmentioning

confidence: 99%

Serum Total Cholinesterase Activity on Admission Is Associated with Disease Severity and Outcome in Patients with Traumatic Brain Injury

Zhang

et al. 2015

PLoS ONE

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BackgroundTraumatic brain injury (TBI) is one of the leading causes of neurological disability. In this retrospective study, serum total cholinesterase (ChE) activities were analyzed in 188 patients for diagnostic as well as predictive values for mortality.Methods and FindingsWithin 72 hours after injury, serum ChE activities including both acetylcholinesterase and butyrylcholinesterase were measured. Disease severity was evaluated with Acute Physiology and Chronic Health Evaluation (APACHE) II score, Glasgow Coma Score, length of coma, post-traumatic amnesia and injury feature. Neurocognitive and functional scores were assessed using clinical records. Of 188 patients, 146 (77.7%) survived and 42 (22.3%) died within 90 days. Lower ChE activities were noted in the non-survivors vs. survivors (5.94±2.19 vs. 7.04±2.16 kU/L, p=0.023), in septic vs. non-infected patients (5.93±1.89 vs. 7.31±2.45 kU/L, p=0.0005) and in patients with extremely severe injury vs. mild injury (6.3±1.98 vs. 7.57±2.48 kU/L, p=0.049). The trajectories of serum ChE levels were also different between non-survivors and survivors, septic and non-infected patients, mild and severely injured patients, respectively. Admission ChE activities were closely correlated with blood cell counts, neurocognitive and functional scores both on admission and at discharge. Receiver operating characteristic analysis showed that the area under the curve for ChE was inferior to that for either APACHE II or white blood cell (WBC) count. However, at the optimal cutoff value of 5 kU/L, the sensitivity of ChE for correct prediction of 90-day mortality was 65.5% and the specificity was 86.4%. Kaplan-Meier analysis showed that lower ChE activity (<5 kU/L) was more closely correlated with poor survival than higher ChE activity (>5 kU/L) (p=0.04). After adjusting for other variables, ChE was identified as a borderline independent predictor for mortality as analyzed by Binary logistic regression (P=0.078).ConclusionsLowered ChE activity measured on admission appears to be associated with disease severity and outcome for TBI patients.

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“…In this context, Charoenying et al (2011) showed that in the MOLT3 cell line, the exposure to paraoxon (100, 500 and 1000 M) for 48 h induces an increase in the expression of the N-AChE region (N-terminus acetylcholinesterase). The biological meaning of the AChE alteration can be related with what Da Silva et al (2011) published. In this study, they reported a significant increase in the expression of AChE in lymphocytes during events of acute infections.…”

Section: Discussionmentioning

confidence: 96%

Effects of diazinon on the lymphocytic cholinergic system of Nile tilapia fish (Oreochromis niloticus)

Toledo-Ibarra

Díaz-Reséndiz

Pavón

et al. 2016

Veterinary Immunology and Immunopathology

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Trypanosoma evansi: Immune response and acetylcholinesterase activity in lymphocytes from infected rats

Cited by 13 publications

References 45 publications

Activity of cholinesterases and adenosine deaminase in blood and serum of rats experimentally infected withTrypanosoma cruzi

Activity of cholinesterases and adenosine deaminase in blood and serum of rats experimentally infected withTrypanosoma cruzi

Serum Total Cholinesterase Activity on Admission Is Associated with Disease Severity and Outcome in Patients with Traumatic Brain Injury

Effects of diazinon on the lymphocytic cholinergic system of Nile tilapia fish (Oreochromis niloticus)

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