TSA protects H9c2 cells against thapsigargin-induced apoptosis related to endoplasmic reticulum stress-mediated mitochondrial injury

Li, Zhiping; Liu, Yan; Dai, Xinlun; Zhou, Qinglong; Liu, Xueli; Li, Zeyu; Chen, Xia

doi:10.1016/j.jsps.2017.04.029

Cited by 5 publications

(1 citation statement)

References 35 publications

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“…The ER is the major reservoir for intracellular Ca 2+ and is considered the dominant modulator of Ca 2+ homeostasis [23]. TG, an inducer of ER stress [24], inhibit isoform of the Ca 2+ -ATPase in ER, and elevates the free Ca 2+ content [25]. In the present study, we showed that MES23.5 cells were treated with TG (0, 0.1 μM or 1 μM) for 24 h, CHOP and XBP-1s protein level in the TG-treated group were significantly higher than that in the control group.…”

Section: Discussionmentioning

confidence: 99%

TG could Modulate FPN1 in MES 23.5 Cells by Hepcidin

jun¹

2018

ijSciences

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Objective To observe the effects of thapsigargin (TG) on ferroportin1 (FPN1) in MES23.5 dopaminergic cell. Methods MES23.5 cells were treated with TG (0.1 μM or 1 μM) for 24h. the mRNA levels of hepcidin were measured using real-time PCR; The protein levels of CHOP, XBP-1s and FPN1 were measured using western blots. Results CHOP and XBP-1s expression was significantly higher in the TG-treated group than that in the control group; hepcidin mRNA levels in TG-treated group were up-regulated, this increase was to a larger extent in the 1 μM TG group when compare with the 0.1 μM TG group; however, FPN1 expression was significantly lower in the TG-treated group, this decrease was to a larger extent in the 1 μM TG group when compare with the 0.1 μM TG group. Conclusion TG could modulate FPN1 in MES23.5 cells by hepcidin.

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Section: Discussionmentioning

confidence: 99%

TG could Modulate FPN1 in MES 23.5 Cells by Hepcidin

jun¹

2018

ijSciences

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Trichostatin A increases BDNF protein expression by improving XBP-1s/ATF6/GRP78 axis in Schwann cells of diabetic peripheral neuropathy

Zhang

Jia

et al. 2021

Biomedicine & Pharmacotherapy

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Icarrin prevents cardiomyocyte apoptosis in spontaneously hypertensive rats by inhibiting endoplasmic reticulum stress pathways

Qian

Zhu

et al. 2021

Journal of Pharmacy and Pharmacology

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Objectives This study aimed to explore whether icarrin (ICA) can protect cardiomyocytes from hypertension-induced damage by inhibiting endoplasmic reticulum stress (ERS). Methods Spontaneously hypertensive rats (SHRs) were orally administered water or ICA at 10, 20 and 40 mg/kg once daily for 12 weeks, and Wistar–Kyoto (WKY) rats were used as control. Changes in the growth and blood pressure of rats were assessed. Cardiac function was determined by ultrasound and the left ventricle mass was calculated. Myocardial tissue structure was assessed by haematoxylin and eosin staining, cardiomyocyte apoptosis was observed by TUNEL staining and the expression of ERS-related proteins was determined by western blotting. Results In the SHR group, blood pressure was significantly high, left ventricular function decreased and left ventricular mass index increased. Additionally, left ventricular cardiomyocyte hypertrophy, disordered myofilament arrangement and increased cardiomyocyte apoptosis were observed by histological staining. ERS-induced proteins associated with apoptosis, including GRP78, PERK, ATF-6, ATF-4, CHOP, DR5, Caspase 12, c-JUN and ASK-1 were found to be highly expressed. ICA treatment reduced blood pressure and regulated the expression of proteins induced by ERS. Cardiomyocyte apoptosis decreased and left ventricular function improved. Conclusions ICA can inhibit ERS-induced apoptosis of cardiomyocytes and protect ventricular function in SHR.

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TSA protects H9c2 cells against thapsigargin-induced apoptosis related to endoplasmic reticulum stress-mediated mitochondrial injury

Abstract: TSA decreases ERS-induced cell apoptosis and mitochondrial injury may related to enhance the location of GRP78 in mitochondrion.

Cited by 5 publications

References 35 publications

TG could Modulate FPN1 in MES 23.5 Cells by Hepcidin

TG could Modulate FPN1 in MES 23.5 Cells by Hepcidin

Trichostatin A increases BDNF protein expression by improving XBP-1s/ATF6/GRP78 axis in Schwann cells of diabetic peripheral neuropathy

Icarrin prevents cardiomyocyte apoptosis in spontaneously hypertensive rats by inhibiting endoplasmic reticulum stress pathways

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