Tuber transmission of ‘Candidatus Liberibacter solanacearum’ and its association with zebra chip on potato in New Zealand

Pitman, Andrew R.; Drayton, G.M.; Kraberger, Simona; Genet, R. A.; Scott, Ian

doi:10.1007/s10658-010-9702-1

Cited by 58 publications

(46 citation statements)

References 26 publications

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“…CaLso is transmitted from potato mother tubers to growing plants and to progeny tubers (Pitman et al, 2011) and is a carrot seed borne pathogen , which poses a high risk of introduction of the bacterium into new areas in which contaminated carrot seeds are commercialized. The bacterium is also horizontally and naturally spread by different psyllid species (Hemiptera: Psylloidea) following establishment (Hansen et al, 2008;Alfaro-Fernández et al, 2012;Nissinen et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Transmission of ‘Candidatus Liberibacter solanacearum’ by Bactericera trigonica Hodkinson to vegetable hosts

Teresani

Hernández

Bertolini

et al. 2018

Span. j. agric. res.

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The bacterium 'Candidatus Liberibacter solanacearum' is a recent plant pathogen of several crops in Solanaceae and Apiaceae and is associated with economically important diseases. The bacterium is a carrot seed borne pathogen that can also be transmitted from potato mother tubers and by psyllid vectors. The psyllid Bactericera trigonica Hodkinson was described carrying CaLso associated with vegetative disorders in carrot and celery crops in Spain and its competence to transmit this phloem-limited bacterium among vegetables is currently being investigated. Here electrical penetration graphs showed that B. trigonica fed in the phloem of carrot and celery and probed the phloem in potato, but not in tomato plants. The bacterium was efficiently transmitted to carrot and celery plants when either single B. trigonica or groups of ten fed on these species. An inoculation access period of 24 hours was sufficient for a single B. trigonica to transmit the bacterium to carrot (67.8%), celery (21.1%) and eventually to potato and tomato (6.0%). Higher transmission rates were obtained with 10 individuals on celery (100%), carrot (80%), potato (10%) and tomato (10%). Bactericera trigonica laid eggs, and the hatched nymphs develop into adult on carrot and celery, but not on potato and tomato. CaLso was detected in 20% of the eggs laid by females carrying the bacterium. The results confirmed that B. trigonica is a vector of the bacterium to carrot and celery, and it is discussed the potential role of this psyllid in the transmission of the pathogen to potato and tomato plants.Additional keywords: electrical penetration graph; haplotype E; psyllids; real-time PCR; transovarial passage.

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Section: Introductionmentioning

confidence: 99%

Transmission of ‘Candidatus Liberibacter solanacearum’ by Bactericera trigonica Hodkinson to vegetable hosts

Teresani

Hernández

Bertolini

et al. 2018

Span. j. agric. res.

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“…However, the metabolic cost of the copy number may play a role as evidenced by apparently low titre in plant hosts during periods of low growth but higher titre during growth flushes of citrus [50,51] or after storage in seed tubers of potatoes [52,53]. An extreme of survival in root systems, even after the tree has been killed [54], indicates the potential for long term survival even in a dormant host.…”

Section: Discussionmentioning

confidence: 99%

Intragenomic homogeneity on Liberibacter 16S rDNA confirms phylogeny and explains ecological strategy

Nelson

Wulff

Bové³

2015

Preprint

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Three of the five currently recognized "Candidatus Liberibacter" spp., "Ca. L. asiaticus" (Las), "Ca. L. americanus" (Lam) and "Ca. L. solanacearum", and the newly erected genus Liberibacter species, L. crescens (Lcr), have had their genomes sequenced. In all four cases there are three homogeneous copies of the 16S rRNA gene, one present as the reverse complement of the other two. 16S intragenic homogeneity is common within the α-Proteobacteria. The presence of three 16S rRNA copies indicates an advantage for a rapid response of population increase to favourable growth conditions. The metabolic cost of carrying multiple copies is avoided during periods of low cellular activity as this situation occurs at low temperatures, for example overwintering in deciduous plants or in a dormant insect host. A large insertion in the 16S rDNA sequence of three species compared to the other three species indicates a dichotomy in the Liberibacter genus and provides a phylogenetic signal of closeness to the proximal node within the Rhizobiaceae. In spite of similar symptoms in Citrus crops associated with Lam and Las infections, these species belong on either side of this dichotomy, thus confirming Lam as phylogenetically closer to the proximal node than Las.

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“…When infected with CLs, the bacteria spread throughout the plant and can infect daughter tubers. Because of this, to prevent the spread the disease, it is necessary to destroy an entire crop where zebra chip is found (Pitman et al 2010).…”

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confidence: 99%

Low-Level Detection ofCandidatusLiberibacter Solanacearum inBactericera cockerelli(Hemiptera: Triozidae) by 16s rRna Pyrosequencing: Table 1.

2013

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Accurate detection and quantification of Candidatus Liberibacter solanacearum (CLs), the putative causal agent of zebra chip disease of potato (Solanum tuberosum L.), in the potato psyllid, Bactericera cockerelli (Sulc), has become necessary to better understand the biology of the disease cycle. Studies on the transmission efficiency of potato psyllids have shown inconsistencies with field surveys. There have also been reports of laboratory colonies inexplicably losing and regaining CLs infection as detected by polymerase chain reaction (PCR). Until now, DNA primers were used to detect CLs in potato psyllid tissue using conventional polymerase chain reaction (PCR) and gel electrophoresis or by real-time quantitative PCR. In this study, CLs was detected using bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) at levels identifiable by PCR, and low levels, including samples with only one cell of CLs. Potato psyllids with <300 pyrosequencing reads did not show positive using conventional PCR. These results indicate that the currently accepted PCR diagnostic technique produces false negatives due to detection limits higher than what is generally present in field collected psyllids, and also provides an explanation as to why laboratory colonies seem to lose and regain CLs infection.

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Tuber transmission of ‘Candidatus Liberibacter solanacearum’ and its association with zebra chip on potato in New Zealand

Cited by 58 publications

References 26 publications

Transmission of ‘Candidatus Liberibacter solanacearum’ by Bactericera trigonica Hodkinson to vegetable hosts

Transmission of ‘Candidatus Liberibacter solanacearum’ by Bactericera trigonica Hodkinson to vegetable hosts

Intragenomic homogeneity on Liberibacter 16S rDNA confirms phylogeny and explains ecological strategy

Low-Level Detection ofCandidatusLiberibacter Solanacearum inBactericera cockerelli(Hemiptera: Triozidae) by 16s rRna Pyrosequencing: Table 1.

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