Tumor necrosis factor-alpha (TNF-alpha) potently enhances in vitro macrophage production from primitive murine hematopoietic progenitor cells in combination with stem cell factor and interleukin-7: novel stimulatory role of p55 TNF receptors

Fahlman, Cecilia; Jacobsen, Frede W.; Veiby, O. Petter; McNiece, Ian; Blomhoff, Heidi Kiil; Jacobsen, Sten Eirik W.

doi:10.1182/blood.v84.5.1528.1528

Cited by 30 publications

(13 citation statements)

References 28 publications

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“…Once released, TNF-α and IL-1 act on different target cells to promote the proliferation, activation, differentiation, and survival of macrophages (Witsell & Schook, 1992; Fahlman et al, 1994; Conte et al, 2006) and all these effects enhance proinflammatory responses during sepsis, we confirmed an increase in GM-CSF levels at 17 and 48 h in the LPS group, when compared to the CTL group. GM-CSF levels only decreased at 17 h in the LPSOmg group, but were not statistically different from the LPS group at 17 h in the other treatment groups.…”

Section: Resultssupporting

confidence: 63%

Comparison of olive leaf, olive oil, palm oil, and omega-3 oil in acute kidney injury induced by sepsis in rats

Ramos

Oliveira

Barros

et al. 2019

PeerJ

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BackgroundHypotension, increased production of reactive oxygen species, and inflammation are all observed in experimental models of sepsis induced by lipopolysaccharide (LPS).PurposeThe aim of this study was to evaluate the effects of an ethanolic extract of Brazilian olive leaf (Ex), Brazilian olive oil (Olv), Ex + Olv (ExOlv), and palm oil (Pal) in comparison to the effects of omega-3 fish oil (Omg) in a rat model of sepsis-induced acute kidney injury.MaterialsWistar rats were divided into seven groups (seven per group), which were either untreated (control) or treated with LPS, LPS + Ex, LPS + ExOlv, LPS + Olv, LPS + Omg, or LPS + Pal.ResultsLower values of creatinine clearance and blood pressure were observed in the LPS-treated group, and these values were not affected by Ex, Olv, ExOlv, Pal, or Omg treatment. Mortality rates were significantly lower in rats exposed to LPS when they were also treated with Ex, ExOlv, Olv, Pal, or Omg. These treatments also decreased oxidative stress and inflammation (Tumor necrosis factor alpha, interleukin-1 beta) and increased interleukin-10 levels and cell proliferation, which were associated with decreased apoptosis in kidney tissue.ConclusionEx and Pal treatments were beneficial in septic rats, since they increased survival rate and did not aggravate inflammation. However, the most effective treatments for septic rats were Olv in comparison to Omg. These natural food substances could enable the development of effective therapeutic interventions to sepsis.

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Section: Resultssupporting

confidence: 63%

Comparison of olive leaf, olive oil, palm oil, and omega-3 oil in acute kidney injury induced by sepsis in rats

Ramos

Oliveira

Barros

et al. 2019

PeerJ

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“…However, the rescue effect we observed was partial. In fact, higher concentrations of TNF-α (10-20 ng/ml) even reduced the recovery of Sirt6KO BMDCs, which could reflect the tendency of high amounts of TNF-α to switch CD34 + progenitor differentiation towards the macrophage lineage [ 31 ]. The immuno-phenotype of these BM cultures (CD11c − CD11b + LyC + LyG − , data not shown) indeed supports this notion.…”

Section: Discussionmentioning

confidence: 99%

Sirt6 regulates dendritic cell differentiation, maturation, and function

Lasigliè

Boero

Bauer

et al. 2016

Aging

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Dendritic cells (DCs) are antigen-presenting cells that critically influence decisions about immune activation or tolerance. Impaired DC function is at the core of common chronic disorders and contributes to reduce immunocompetence during aging. Knowledge on the mechanisms regulating DC generation and function is necessary to understand the immune system and to prevent disease and immunosenescence. Here we show that the sirtuin Sirt6, which was previously linked to healthspan promotion, stimulates the development of myeloid, conventional DCs (cDCs). Sirt6-knockout (Sirt6KO) mice exhibit low frequencies of bone marrow cDC precursors and low yields of bone marrow-derived cDCs compared to wild-type (WT) animals. Sirt6KO cDCs express lower levels of class II MHC, of costimulatory molecules, and of the chemokine receptor CCR7, and are less immunostimulatory compared to WT cDCs. Similar effects in terms of differentiation and immunostimulatory capacity were observed in human monocyte-derived DCs in response to SIRT6 inhibition. Finally, while Sirt6KO cDCs show an overall reduction in their ability to produce IL-12, TNF-α and IL-6 secretion varies dependent on the stimulus, being reduced in response to CpG, but increased in response to other Toll-like receptor ligands. In conclusion, Sirt6 plays a crucial role in cDC differentiation and function and reduced Sirt6 activity may contribute to immunosenescence.

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“…Similarly, TNF‐α inhibits the early stem cells and G‐CSF‐induced progenitor cell proliferation [12]. In contrast, TNF‐α enhances IL‐3 and GM‐CSF‐induced progenitor cell proliferation, and SCF and IL‐7‐induced macrophage proliferation from enriched stem cell populations [10,, 11]. MIP‐1α inhibits primitive stem cells but enhances colony formation in combination with M‐CSF and GM‐CSF [49,, 50], while no effect is seen on GM‐CFC colony formation in combination with G‐CSF [49,, 51].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Enriched Stem Cells in Vivo and in Vitro by the Hemoregulatory Peptide SK&F108636

et al. 1996

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Abstract. Replacement of the labile sulfhydryl group (-SH) of the hemoregulatory peptide monomer pyroGluGluAspCysLys (HP5b) with an isosteric methylene group yields a chemically stable compound, SK&F108636. In this study, we describe the effects of SK&F108636 on highly enriched Lin -Sca1 + hematopoietic stem cells. SK&F108636 significantly reduced the fraction of cycling progenitor cells, granulocyte macrophage colony-forming cells (GM-CFC), in vitro and in vivo. There was no effect on GM-CFC or Mix-CFC colony formation. SK&F108636 significantly inhibited proliferation of high proliferative potential (HPP)-CFC in semisolid agar cultures stimulated by stem cell factor + interleukin 3 (IL-3) + IL-1, but had no effect in cultures stimulated with M-CSF + IL-3 + IL-1. SK&F108636 was shown to act directly on the stem cells since SK&F108636 inhibited proliferation of Lin -Sca1 + cells in single cell assays. Administration of SK&F108636 to lethally irradiated mice transplanted with 2000 Lin -Sca1 + cells significantly inhibited proliferation/differentiation of cells developing into colony forming units-spleen (CFU-S) (preCFU-S) and the reconstitution of HPP-CFC and GM-CFC. There was no effect of SK&F108636 on CFU-S colony formation or mature cell regeneration in bone marrow, spleen and blood. Hence, the hemoregulatory peptide monomer SK&F108636 is a potent primitive stem cell inhibitor in vivo and in vitro. Inhibition of stem cell proliferation by small specific inhibitors may protect hematopoiesis from myelotoxic side effects during chemotherapy treatment.

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Tumor necrosis factor-alpha (TNF-alpha) potently enhances in vitro macrophage production from primitive murine hematopoietic progenitor cells in combination with stem cell factor and interleukin-7: novel stimulatory role of p55 TNF receptors

Cited by 30 publications

References 28 publications

Comparison of olive leaf, olive oil, palm oil, and omega-3 oil in acute kidney injury induced by sepsis in rats

Comparison of olive leaf, olive oil, palm oil, and omega-3 oil in acute kidney injury induced by sepsis in rats

Sirt6 regulates dendritic cell differentiation, maturation, and function

Inhibition of Enriched Stem Cells in Vivo and in Vitro by the Hemoregulatory Peptide SK&F108636

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